鸡血藤总黄酮对坐骨神经损伤大鼠机械痛敏影响的实验研究

医学研究与教育 ›› 2015, Vol. 32 ›› Issue (1): 7-11.

鸡血藤总黄酮对坐骨神经损伤大鼠机械痛敏影响的实验研究

刘仰斌，张志花，陈亚运

赣南医学院，江西赣州 341000

出版日期:2015-02-25 发布日期:2015-02-25
作者简介:刘仰斌（1970—），男，江西赣州人，副教授，硕士，硕士生导师，主要从事免疫细胞与神经疼痛机制研究。E-mail: 15979776628＠163.com。

Experimental study of total flavonoids in Net Cliffbean affecting the mechanical allodynia in CCI rats

LIU Yangbin, ZHANG Zhihua, CHEN Yayun

Gannan Medical University, Ganzhou 341000, China

Online:2015-02-25 Published:2015-02-25

摘要/Abstract

摘要： 目的研究鸡血藤总黄酮对坐骨神经损伤（chronic constriction injury of the sciatic，CCI）大鼠机械痛敏和脊髓谷氨酸含量的影响。方法 40 只大鼠随机均分为 4 组：对照组（腹腔注射生理盐水，不手术）、假手术组（分离坐骨神经但不结扎+腹腔注射生理盐水）、模型组（坐骨神经结扎+腹腔注射生理盐水）、鸡血藤总黄酮处理组（坐骨神经结扎+腹腔注射鸡血藤总黄酮20 g/kg），连续给药4 周。运用机械缩足反射阈值检测术后 3、7、10、14、21、28 d 大鼠机械痛敏。运用高效液相法测定大鼠术后 14、28 d 脊髓背角中谷氨酸的含量。结果与模型组比较，处理组术后第 10、14、21、28 天的机械缩足反射阈值明显升高（P＜0.05, 或 P＜0.01）；在第 14 天及第 28 天观测到鸡血藤总黄酮可显著降低大鼠脊髓背角谷氨酸的含量（P＜0.01）。结论鸡血藤总黄酮可减轻神经病理性痛大鼠的机械痛敏，其机制可能与降低脊髓背角谷氨酸的含量有关。

关键词: 鸡血藤, 总黄酮, 神经病理性痛, 机械痛敏, 谷氨酸

Abstract: Objectivev To study the effects of total flavonoids in Net Cliffbean on the mechanical allodynia and glutamate contents in spinal of chronic constriction injury of the sciatic(CCI) rats. Methods 40 rats were randomly divided into 4 groups: control group, sham operation group, CCI group and Net Cliffbean treatment group, with NomralSaline or drugs, once a day, for 4 weeks. Mechanical withdrawal threshold (MWT) was measured as the sign of mechanical allodynia for 3, 7, 10, 14, 21, 28 day. The lumbar segment of the spinal cord was removed for determination of glutamate contents by high pressure liquid chromatography (HPLC) for 14, 28 day. Results Total flavonoids in Net Cliffbean significantly increased the mechanical withdrawal threshold (MWT) compared with model group (P＜0.05 or P＜0.01), and obviously decreased the content of glutamate in the spinal cord (P＜0.01). Conclusion Total flavonoids in Net Cliffbean can relieve the mechanical allodynia in neuropathic pain rats, its mechanism maybe reduced glutamate content in the spinal cord.

Key words: Net Cliffbean, total flavonoids, neuropathic pain, mechanical allodynia, glutamate

中图分类号:

刘仰斌，张志花，陈亚运. 鸡血藤总黄酮对坐骨神经损伤大鼠机械痛敏影响的实验研究[J]. 医学研究与教育, 2015, 32(1): 7-11.

LIU Yangbin, ZHANG Zhihua, CHEN Yayun. Experimental study of total flavonoids in Net Cliffbean affecting the mechanical allodynia in CCI rats[J]. Medical Reserch and Education, 2015, 32(1): 7-11.

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