溶菌酶稳定的银纳米粒子的合成及稳定性研究

doi:10.3969/j.issn.1674-490X.2014.04.001

医学研究与教育 ›› 2014, Vol. 31 ›› Issue (4): 1-7,19.DOI: 10.3969/j.issn.1674-490X.2014.04.001

• 基础研究 • 下一篇

溶菌酶稳定的银纳米粒子的合成及稳定性研究

赵亚利,杨涛,张英,高红云,席亚楠

河北大学化学与环境科学学院,河北保定,071002

出版日期:2014-08-25 发布日期:2014-08-25
基金资助:
河北省自然科学基金资助项目(B2011201107)；河北大学引进人才科研启动项目基金

Study on the preparation and stability of the lysozyme-stabilized silver nanoparticles

ZHAO Yali,YANG Tao,ZHANG Ying,GAO Hongyun,XI Yanan

Online:2014-08-25 Published:2014-08-25

摘要/Abstract

摘要： 目的：使用蛋白质做稳定剂来合成银纳米粒子，并探讨其稳定性，为其在生物医学领域及日常生活中的应用提供理论指导。方法采用化学还原法一步合成溶菌酶稳定的银纳米粒子，改变稳定性影响因素并观察银纳米溶胶的变化。即加入不同浓度的氯化钠来改变银纳米溶胶的离子强度，用酸或碱来调节溶胶pH，对溶胶进行多次冷冻-解冻循环。并且用柠檬酸稳定的银纳米粒子做对照试验。结果溶菌酶稳定的银纳米粒子对离子强度的变化比较敏感，在10 mmol/L NaCl条件下完全沉淀；在酸性条件下能够稳定存在（pH≤6）；冷冻-解冻循环对其稳定性影响比较小。结论溶菌酶稳定的银纳米粒子的稳定性受周围环境影响较大，在使用及储存时需要加以注意。

关键词: 银纳米粒子, 溶菌酶, 稳定性

Abstract: Objective We have synthesized silver nanoparticles using protein as the stabilizer and discussed their stability to provide theoretical guidance for their application in biomedical ifeld and daily life. Methods We have presented a one-step approach of chemical reduction to prepare the lysozyme-stabilized silver nanoparticles. Results The stability of lysozyme-stabilized silver nanoparticles was sensitive to the change of ionic strength and the nanoparticles precipitated completely in 10 mmol/L NaCl. The lysozyme-stabilized silver nanoparticles were stable under acidic condition (pH≤6). And the freeze-thawing cycle had a little inlfuence on their stability. Conclusion The stability of lysozyme-stabilized silver nanoparticles is greatly inlfuenced by the surrounding conditions. The variation caused by the stability factors should be considered for use and storage of the lysozyme-stabilized silver nanoparticles.

Key words: silver nanoparticles, lysozyme, stability

中图分类号:

O648

赵亚利,杨涛,张英,高红云,席亚楠. 溶菌酶稳定的银纳米粒子的合成及稳定性研究[J]. 医学研究与教育, 2014, 31(4): 1-7,19.

ZHAO Yali,YANG Tao,ZHANG Ying,GAO Hongyun,XI Yanan. Study on the preparation and stability of the lysozyme-stabilized silver nanoparticles[J]. Medical Reserch and Education, 2014, 31(4): 1-7,19.

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溶菌酶稳定的银纳米粒子的合成及稳定性研究

Study on the preparation and stability of the lysozyme-stabilized silver nanoparticles

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