哮喘大鼠肺组织CyclinD1、VEGF、SMA-α表达变化

医学研究与教育 ›› 2013, Vol. 30 ›› Issue (1): 23-28.

哮喘大鼠肺组织CyclinD1、VEGF、SMA-α表达变化

杨卫¹,吴欣¹,赵学琴¹,张泽明¹

河北大学附属医院呼吸内科,河北保定 071000

收稿日期:2016-10-09 修回日期:2016-10-09 出版日期:2013-02-25 发布日期:2013-02-25

Studying the expression of CyclinD1, VEGF, SMA-αin the lung of asthmatic rats

Received:2016-10-09 Revised:2016-10-09 Online:2013-02-25 Published:2013-02-25

摘要/Abstract

摘要： 目的制备哮喘大鼠动物模型,研究哮喘大鼠模型肺组织中细胞周期蛋白D1(CyclinD1)、血管内皮生长因子(VEGF)和平滑肌肌动蛋白-α(SMA-α)的表达变化.方法16只雄性SD大鼠随机分成正常组和哮喘组,每组8只.通过卵清蛋白腹膜腔注射致敏,反复卵清蛋白雾化吸入的方法制备哮喘动物模型.检测大鼠肺功能,HE染色观察气道炎症情况,马松染色观察气道胶原沉积变化,酶联免疫吸附试验检测血清VEGF表达,免疫组化法检测气道SMA-α表达,蛋白印迹法分析肺组织CyclinD1的表达.结果与正常组比较,哮喘组大鼠肺功能减低,气道周围胶原沉积增多,CyclinD1、VEGF和气道SMA-α表达明显增高(P<0.05).哮喘组CyclinD1与气道SMA-α表达呈明显正相关(r=0.68, P<0.05);正常组CyclinD1与气道SMA-α表达呈明显正相关(r=0.68, P<0.05);哮喘组VEGF与胶原表达呈正相关(r=0.56, P<0.05);正常组VEGF与胶原表达呈正相关(r=0.56, P<0.05).结论VEGF和CyclinD1参与调节哮喘气道重塑.

关键词: 支气管哮喘, 细胞周期蛋白D1, 血管内皮生长因子, 气道平滑肌肌动蛋白-α

Abstract: Objective To observe the expression of CyclinD1、VEGF、SMA-α in the lung through making asthmatic modeling rats. Methods Sixteen males SD rats were randomly assigned into control group and asthma group, each group had eight rats. The mice were sensitized ovalbumin, then they were challenged with ovalbumin to establish the asthmatic model. The lung function were detected. The sections were stained with HE to assess the inflammatory cell infiltrates. Masson staining was used to detect the expression of collagen in the mice lungs. VEGF levels in serum were measured by enzyme linked-immunosorbent assay (ELISA). Immunohistochemistry was used to detect the expression of SMA-α in the mice lungs. Western blot were applied to examine the expression of CyclinD1 in the lungs. Results The bronchial provocation test showed that, in the asthmatic group, the average expiratory resistance increased remarkably, the expression of collagen, VEGF, SMA-α and CyclinD1 was obviously increased than that of the control group(P<0.05). There were positive correlation between CyclinD1 with SMA-α in asthmatic modeling rats(r=0.68, P<0.05), as well that were positive correlation in control group(r=0.54, P<0.05). There were positive correlation between VEGF with collagen in asthmatic modeling rats(r=0.56, P<0.05), as well that were positive correlation in control group(r=0.48, P<0.05). Conclusion CyclinD1and VEGF are playing an important role in airway remodeling.

Key words: asthma, CyclinD1, VEGF, SMA-α

中图分类号:

R361

杨卫,吴欣,赵学琴,张泽明. 哮喘大鼠肺组织CyclinD1、VEGF、SMA-α表达变化[J]. 医学研究与教育, 2013, 30(1): 23-28.

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