壁虎醇提物提取优化及体外抗肿瘤初步研究

doi:10.3969/j.issn.1674-490X.2016.05.001

医学研究与教育 ›› 2016, Vol. 33 ›› Issue (5): 1-5.DOI: 10.3969/j.issn.1674-490X.2016.05.001

• 基础医学 • 下一篇

壁虎醇提物提取优化及体外抗肿瘤初步研究

宋佳玉,游颜杰

河南漯河医学高等专科学校, 河南漯河 462002

收稿日期:2016-06-29 出版日期:2016-10-25 发布日期:2016-10-25
作者简介:宋佳玉(1981—),女,河南漯河人,讲师,硕士,主要从事肿瘤药理研究。 E-mail: 2007songjiayu@163.com
基金资助:
河南省医学科技攻关计划项目(200903109)

Preliminary study on the extraction of Gecko extract and its anti-tumor activity

SONG Jiayu,YOU Yanjie

Luohe Medical College, Luohe 462002, China

Received:2016-06-29 Online:2016-10-25 Published:2016-10-25

摘要/Abstract

摘要： 目的优化壁虎醇提物提取工艺,并对壁虎醇提物体外抗肿瘤活性进行初步研究。方法采用超声破碎、乙醇提取法提取壁虎粗提物,在单因素分析的基础上行正交试验。结果料液比为1∶4、超声功率400 W、超声时间30 min、乙醇终浓度55%时为最佳提取工艺,此时壁虎醇提物得率约为6.5%,采用双缩脲法检测该提取物中蛋白含量约为23.15%。结论体外试验表明壁虎醇提物可显著抑制癌细胞的生长,并呈现一定的浓度和时间依赖性。

关键词: 壁虎, 醇提物, 抗肿瘤活性

Abstract: Objective To optimize the extraction process of Gecko extract and preliminarily assess its antitumor activity in vitro.Methods We used approaches of ultrasonication and extraction with ethanol,and performed orthogonal tests upon univariate analysis.Results Our results revealed the optimal extraction conditions as follows:the ratio of material to solvent(1∶4),ultrasonic time(30 min),ultrasonic power(400 W),and final concentration of ethanol(55%).Thus,the yield of Gecko extract was about 6.5% and the total content of protein included was about 23.15%.Conclusion The results showed that exposure to Gecko extract dramatically suppressed the proliferation of esophageal carcinoma EC-109 cells and prostatic cancer PC-3 cells in a dose and time-dependent manner.

Key words: Gecko, extract, antitumor activity

中图分类号:

R965.5

宋佳玉,游颜杰. 壁虎醇提物提取优化及体外抗肿瘤初步研究[J]. 医学研究与教育, 2016, 33(5): 1-5.

SONG Jiayu,YOU Yanjie. Preliminary study on the extraction of Gecko extract and its anti-tumor activity[J]. Medical Reserch and Education, 2016, 33(5): 1-5.

[1]	刘博,程树杰. 黄独乙素对人胃癌SGC-7901细胞及其耐药细胞株体外抑制作用的研究[J]. 医学研究与教育, 2014, 31(3): 6-10.
[2]	杨春柳,李佳,杨更亮. 顺-6-氟-3-氯代亚甲基-硫色满-4-酮的体内抗肿瘤活性研究[J]. 医学研究与教育, 2011, 28(6): 13-18.
[3]	曹志然,王海,唐志远,王永丽. 4种源于虎皮楠的生物碱对人肝癌细胞株HepG-2增殖的影响[J]. 医学研究与教育, 2010, 27(6): 6-10.

壁虎醇提物提取优化及体外抗肿瘤初步研究

Preliminary study on the extraction of Gecko extract and its anti-tumor activity

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 3

编辑推荐

Metrics

本文评价