Establishment of nanovaccines for dual-delivery of antigens and CpG nucleic acid

doi:10.3969/j.issn.1674-490X.2016.04.001

Medical Reserch and Education ›› 2016, Vol. 33 ›› Issue (4): 1-6.DOI: 10.3969/j.issn.1674-490X.2016.04.001

Establishment of nanovaccines for dual-delivery of antigens and CpG nucleic acid

LI Zhenhua¹, YIN Xuan², WANG Yelian², ZHAO Baocheng², DUAN Fei¹

1. College of Chemistry& Environmental Science, Hebei University, Baoding 071002, China; 2. The First Center Hospital of Baoding, Baoding 071000, China

Received:2016-07-01 Online:2016-08-25 Published:2016-08-25

Abstract

Abstract: Objective To establish effective nonvaccines to solve the problems of low internalized efficiency by antigen presenting cell, low immune activity and easy to be degraded in vivo. Methods Mesoporous silica nanoparticles(MSN)were chosen as the core for antigens(ovalbumin, OVA)loading. Then metal organic frameworks(MOFs)formed by Eu³⁺ and guanine nucleotide(GMP)were coated on the surface of MSN. After adsorption of CpG oligonucleotides, we constructed pH-responsive nanovaccines for dual-delivery of antigens and CpG. Results The loading capability of mesoporous silica was 20%, and the release rate of antigens could reach to 55% after incubation for 24 h under acidic conditions. The results of transmission electron microscope indicated that MOFs were coated on the surface of MSN, the loading capability of nanocarriers for CpG was 1.8×10^-6 mol of nucleic acid per mg of nanoparticles,nanocarriers showed low cytotoxicity analyzed by MTT. Conclusion A novel nanovaccine is constructed and the introduction of MOFs can protect the antigens from degradation and enhance the adsorption rate of nanocarriers. Furthermore, antigens can be released under acidic conditions, which can enhance the immune response in the future.

Key words: protein-based vaccines, nanocarriers, pH-responsive, CpG nucleic acid

CLC Number:

R392.11

LI Zhenhua, YIN Xuan, WANG Yelian, ZHAO Baocheng, DUAN Fei. Establishment of nanovaccines for dual-delivery of antigens and CpG nucleic acid[J]. Medical Reserch and Education, 2016, 33(4): 1-6.

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Establishment of nanovaccines for dual-delivery of antigens and CpG nucleic acid

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