doi:10.3969/j.issn.1674-490X.2021.02.001

Abstract

Abstract: Objective To provide a reliable primary hepatocyte steatosis cell model for the pathogenesis, prevention and treatment of non-alcoholic fatty liver disease(NAFLD).Methods Primary mouse hepatocytes were isolated by Seglen two-step perfusion method and its improved method.After cultured in vitro for 48 hours, different concentrations of free fatty acid(FFA)mixture ［oleic acid(OA)∶palmitic acid(PA)=2∶1］ were induced for 24 hours.The lipid droplet deposition in hepatocytes was observed by oil red O staining and the content of triglyceride(TG)in cells was determined by automatic biochemical analyzer.Results Compared with the control group, there were red lipid droplets in hepatocytes in the model group, and the concentration of FFA mixture was dose-dependent, and the content of TG in hepatocytes in the model group was significantly higher than that in the control group(P<0.05), and increased with the increase of the concentration of FFA, suggesting that the lipid deposition in hepatocytes increased with the increase of FFA concentration.Conclusion The primary hepatocyte steatosis cell model is successfully established, which provides a reliable cell model for the study of non-alcoholic fatty liver disease.

Key words: nonalcoholic fatty liver disease(NAFLD), primary hepatocytes, hepatic steatosis cell model

CLC Number:

R363.2

References

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