红色LED光源对肝损伤大鼠肝再生影响的研究

doi:10.3969/j.issn.1674-490X.2014.02.004

医学研究与教育 ›› 2014, Vol. 31 ›› Issue (2): 12-16.DOI: 10.3969/j.issn.1674-490X.2014.02.004

红色LED光源对肝损伤大鼠肝再生影响的研究

孙建钢¹,刘式浩¹,董宏伟¹,侯陈宁¹,谢盈慧¹,刘子豪¹,王欣悦¹,纪刊¹,崔妍²,李莉²

1.河北大学临床医学院,河北保定,071000
2.河北大学基础医学院,河北保定,071000

收稿日期:2016-09-29 修回日期:2016-09-29 出版日期:2014-04-25 发布日期:2014-04-25

Effects of red LED lights on the regeneration of liver injury in rats

SUN Jiangang¹,LIU Shihao¹,DONG Hongwei¹,HOU Chenning¹,XIE Yinghui¹,LIU Zihao¹,WANG Xinyue¹,JI Kan¹,CUI Yan²,LI Li²

Received:2016-09-29 Revised:2016-09-29 Online:2014-04-25 Published:2014-04-25

摘要/Abstract

摘要： 目的：应用红色LED光源照射肝损伤大鼠，观察其对肝损伤大鼠肝脏再生情况的影响。方法将50只健康成年SD大鼠随机分为5组，每组10只，即空白对照组、模型组、10 min照射组、20 min照射组、30 min照射组。各组大鼠4周后腹主动脉采血检测谷丙转氨酶和谷草转氨酶，取肝脏称重并计算再生叶占肝总质量比例，用显微镜观察再生叶肝细胞的再生情况。结果各治疗组谷丙转氨酶均低于模型组（P均<0.05），谷草转氨酶30 min照射组低于模型组（P<0.05）；病理结果显示，各治疗组大鼠肝细胞再生较模型组明显；再生叶占肝总质量比例均较模型组增大（P均<0.05）。结论红光可促进肝损伤后肝组织的再生，并有助于肝损伤大鼠肝功能的恢复。

关键词: 发光二极管, 红光, 肝损伤, 肝再生, 肝功能

Abstract: Objective To observe the effects of red light source on the liver regeneration of liver injury in rats. Methods Fifty experimental SD rats were randomly divided into five groups, normal control group, model group, 10 min irradiation group, 20 min irradiation group, and 30 min irradiation group, with 10 rats in each group. The blood sampling was undergone to test alanine aminotransferase and aspartate aminotransferase of all groups after 4 weeks. The livers were taken out and weighed to calculate the proportion of regeneration liver lobes weight. The liver tissues were taken to observe the regenerated liver cells of regeneration leaves with microscope. Results The alanine aminotransferase level of each treatment group was lower than that of model group (P<0.05). The aspartate aminotransferase level of the rats in 30 min irradiation group was decreased than those of model group (P<0.05). The results of pathology showed the liver cell regeneration of the treatment group was signiifcantly better than the model group (P<0.05). Compared with the model group, the proportion of the regeneration liver lobe in each treatment group was signiifcantly increased (P<0.05). Conclusion Red light could promote the regeneration capacity of the damaged liver, and help liver function recover of rats with liver injury.

Key words: light emitting diode, red light, liver injury, liver regeneration, liver function

中图分类号:

R363

孙建钢,刘式浩,董宏伟,侯陈宁,谢盈慧,刘子豪,王欣悦,纪刊,崔妍,李莉. 红色LED光源对肝损伤大鼠肝再生影响的研究[J]. 医学研究与教育, 2014, 31(2): 12-16.

SUN Jiangang,LIU Shihao,DONG Hongwei,HOU Chenning,XIE Yinghui,LIU Zihao,WANG Xinyue,JI Kan,CUI Yan,LI Li. Effects of red LED lights on the regeneration of liver injury in rats[J]. Medical Reserch and Education, 2014, 31(2): 12-16.

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红色LED光源对肝损伤大鼠肝再生影响的研究

Effects of red LED lights on the regeneration of liver injury in rats

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