不同受体激动剂诱发大鼠去内皮尾动脉环的收缩反应

doi:10.3969/j.issn.1674-490X.2023.05.003

医学研究与教育 ›› 2023, Vol. 40 ›› Issue (5): 15-21.DOI: 10.3969/j.issn.1674-490X.2023.05.003

不同受体激动剂诱发大鼠去内皮尾动脉环的收缩反应

邓童^1,2,甘璐¹,李璐¹,钟聪敏³,范建辉⁴

1.河北大学附属医院麻醉科, 河北保定 071000;
2.河北大学临床医学院, 河北保定 071000;
3.保定市第一医院老年医学科, 河北保定 071000;
4.保定市满城区第四医院麻醉科, 河北保定 072150

收稿日期:2023-05-08 出版日期:2023-10-25 发布日期:2023-10-26
通讯作者: 李璐(1972—),女,河北阜平人,主任医师,博士,硕士生导师,主要从事临床麻醉及心血管药理学研究。E-mail: lilu20052203@163.com
作者简介:邓童(1997—),男,四川宜宾人,在读硕士,主要从事临床麻醉及心血管药理学研究。 E-mail: foreverdtll@163.com
基金资助:
河北省卫生厅医学科学研究课题(20211165);保定市科学技术研究与发展计划项目(2041ZF326)

Received:2023-05-08 Online:2023-10-25 Published:2023-10-26

摘要/Abstract

摘要： 目的测定不同受体激动剂对大鼠去内皮尾动脉环收缩反应,推测外周阻力血管不同受体介导血管张力的作用。方法制备大鼠离体去内皮尾动脉血管环,测定不同浓度去甲肾上腺素(noradrenaline, NA)、α,β-亚甲基三磷酸腺苷(α,β-MeATP)和尿苷腺苷四磷酸盐(uridine adenosine tetraphosphate, Up4A)诱发的血管收缩反应。结果三种受体激动剂均可产生浓度依赖性收缩反应,反应强度为NA>α,β-MeATP>Up4A,10 μmol/L NA、α,β-MeATP和Up4A诱发的尾动脉环收缩值分别为KCl最大收缩反应的(95.26 ± 14.57)%、(62.28 ± 6.98)%和(12.16± 2.19)%。结论肾上腺素能受体和嘌呤能受体在外周阻力血管张力调节方面具有重要作用;具有嘧啶和嘌呤结构的二核苷酸Up4A在介导外周阻力血管张力方面作用较弱。外周阻力血管平滑肌不同受体介导血管张力的不同可能对血液再分配、保障重要脏器血液供应方面具有重要意义。

关键词: 受体激动剂, 血管收缩, 嘌呤受体, 肾上腺素受体

Abstract: Objective To measure the contractive response of different receptor agonists on the endothelium-denuded caudal artery rings in rats and evaluate the function of different receptors in peripheral resistant vessels. Methods The rat isolated caudal artery rings were prepared, and the vasoconstriction induced by various concentrations of noradrenaline(NA), α,β-MeATP and uridine adenosine tetraphosphate(Up4A)was measured. Results The three receptor agonists could produce concentration-dependent contraction, and the response intensity was NA>α, β-MeATP>Up4A, the values of contraction induced by 10 μmol/L NA, α, β-MeATP and Up4A in caudal artery rings were(95.26 ±14.57)%,(62.28 ±6.98)% and(12.16 ±2.19)% of the maximum contraction induced by KCl, respectively. Conclusion Adrenergic receptors and purinergic receptors may play important roles in the regulation of peripheral resistant vascular tension. The dinucleotide Up4A with pyrimidine and purine structures has the weaker role in mediating peripheral resistant vascular tension. The different vascular tension mediated by different receptors on the peripheral resistance vascular smooth muscle may have significant implications for blood redistribution and ensuring blood supply to important organs.

Key words: receptor agonist, vasoconstriction, purine receptor, adrenoceptor

中图分类号:

R544.1

邓童,甘璐,李璐,钟聪敏,范建辉. 不同受体激动剂诱发大鼠去内皮尾动脉环的收缩反应[J]. 医学研究与教育, 2023, 40(5): 15-21.

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不同受体激动剂诱发大鼠去内皮尾动脉环的收缩反应

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