丹七对兔心肌缺血再灌注损伤保护作用的研究

doi:10.3969/j.issn.1674-490X.2013.06.003

医学研究与教育 ›› 2013, Vol. 30 ›› Issue (6): 13-16.DOI: 10.3969/j.issn.1674-490X.2013.06.003

丹七对兔心肌缺血再灌注损伤保护作用的研究

杨琳¹,周玉娟²,李少春²,于柏青¹,刘福林³,周程³

1.河北大学研究生学院,河北保定,071000
2.河北大学基础医学院,河北保定,071000
3.河北大学附属医院,河北保定,071000

收稿日期:2016-10-09 修回日期:2016-10-09 出版日期:2013-12-25 发布日期:2013-12-25

Study on the protective effects of Danqi in myocardial ischemia reperfusion injury in rabbits

YANG Lin¹,ZHOU Yujuan²,LI Shaochun²,YU Baiqing¹,LIU Fulin³,ZHOU Cheng³

Received:2016-10-09 Revised:2016-10-09 Online:2013-12-25 Published:2013-12-25

摘要/Abstract

摘要： 目的：观察丹七对兔心肌缺血再灌注损伤的保护作用。方法18只兔随机分为三组：假手术组、模型组、丹七干预组。末次给药后1 h，采用结扎兔左冠状动脉前降支的方法制备缺血再灌注损伤模型。运用BL-420F生物机能实验系统记录各组动物左心功能指标的变化；于再灌注120 min 后颈总动脉取血，比色法测定血清中肌酸激酶（CK）、一氧化氮合酶（NOS）的活性及一氧化氮（NO）含量的变化。结果与假手术组比较，模型组中左心功能指标左心室收缩压（LVSP）、左心室内压最大上升速率（+dp/dtmax）、左心室内压最大下降速率（-dp/dtmax）的数值明显降低，左心室舒张末压（LVEDP）的数值明显升高（P＜0.01）；血清中CK的活性明显升高，NOS 的活性及 NO 的含量明显降低（P＜0.01）。与模型组比较，丹七干预组中 LVSP、+dp/dtmax、-dp/dtmax的数值明显升高，LVEDP 的数值明显降低（P＜0.05），血清中CK的活性明显降低，NOS的活性及NO的含量明显升高（P＜0.05）。结论丹七对兔心肌缺血再灌注损伤具有保护作用。

关键词: 丹七, 心肌, 缺血再灌注损伤

Abstract: Objective To study the protective effects of Danqi on myocardial ischemia reperfusion injury in rabbits. Methods Eighteen rabbits were divided into three groups randomly: sham operation group, model group, Danqi intervention group. The operation was performed after the last perfusion. Models of ischemia reperfusion injury were established with the branch-ligation of left coronary artery. The indexes of left heart function were recorded by BL-420F biological function experiment system. After reperfusion 120 minutes, blood samples from common carotid artery were collected and the activity of creatine kinase(CK), nitric oxide synthase(NOS) were tested, and the contents of nitric oxide(NO) were examined with colorimetric method. Results Compared with the sham operation group, the levels of left ventricular systolic blood pressure(LVSP) in the model group, left heart interior maximum pressure rise rate(+dp/dtmax), left heart indoor pressure maximum decline rate(-dp/dtmax) were significantly decreased, the levels of left ventricular end-diastolic pressure(LVEDP ) were significantly increased(P<0.01), the activity of CK was significantly increased, the activity of NOS and the content of NO were signiifcantly decreased(P<0.01). Compared with the model group, the levels of LVSP,+dp/dtmax,-dp/dtmax in Danqi intervention group were signiifcantly increased, the levels of LVEDP were signiifcantly decreased(P<0.05);the activity of CK was signiifcantly decreased, the activity of NOS and the content of NO were signiifcantly increased(P<0.05). Conclusion Danqi has protective effects on myocardial ischemia reperfusion injury in rabbits.

Key words: Danqi, myocardial, ischemia reperfusion injury

中图分类号:

R363

杨琳,周玉娟,李少春,于柏青,刘福林,周程. 丹七对兔心肌缺血再灌注损伤保护作用的研究[J]. 医学研究与教育, 2013, 30(6): 13-16.

YANG Lin,ZHOU Yujuan,LI Shaochun,YU Baiqing,LIU Fulin,ZHOU Cheng. Study on the protective effects of Danqi in myocardial ischemia reperfusion injury in rabbits[J]. Medical Reserch and Education, 2013, 30(6): 13-16.

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丹七对兔心肌缺血再灌注损伤保护作用的研究

Study on the protective effects of Danqi in myocardial ischemia reperfusion injury in rabbits

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