关键词: 鼻咽癌, 放射疗法, 药物疗法, T 细胞亚群, 流式细胞术

Abstract: Objective To explore the relationships between changes of immune functions in patients with nasopharyngeal carcinoma(NPC)pre-chemoradiotherapy and post chemoradiotherapy. Methods The flow cytometry(FACS Calibur) was used to detect the blood T, B Lymphocytes subsets of 60 healthy check-ups and 60 NPC patients before and after chemoradiotherapy. The immune indexes of changes before and after the radiotherapy were analyzed and their test values were compared. Results There were no significant differencesin CD3+, CD16+56+, CD19+(t were 1.567; 0.971; 1.561, P were 0.078; 0.392; 0.240)between the healthy check-ups and the NPC patients before chemoradiotherapy. CD4+、CD4+/CD8+ had a downward trend(t were2.389; 2.358, P were 0.01, 0.01)while CD8+ had an upward trend (t=-2.342, P=0.019), which was statistically significant. CD3+, CD4+, CD4+/CD8+ were significantly different before and after the chemoradiotherapy,CD3+, CD4+, CD4+/CD8+ gradually down, which had significant differences(t were -7.163, -11.217, 3.817 P<0.01); CD8+, CD16+56+ gradually up,which had significant differences(t were -8.271, 4.383, P were 0.010.001).There was no significant difference in CD19+ (t=-0.986, P=0.323).Conclusions The immune functions get lower in the NPC patients after chemoradiotherapy and can be estimated by the test of the blood T, B Lymphocytes subsets.

Key words: nasopharyngeal, radiotherapy, surgery, T lymphocytesubsets, flow cytome-try