早期吸入布地奈德对过敏性哮喘模型大鼠肺功能、骨桥蛋白及α-SMA的影响

doi:10.3969/j.issn.1674-490X.2020.02.005

医学研究与教育 ›› 2020, Vol. 37 ›› Issue (2): 31-36.DOI: 10.3969/j.issn.1674-490X.2020.02.005

早期吸入布地奈德对过敏性哮喘模型大鼠肺功能、骨桥蛋白及α-SMA的影响

杨卫¹,许晓云¹,监士宁¹,王海洋²,吴欣¹

1.保定市第一中心医院呼吸内二科, 河北保定 071000;
2.河北大学附属医院, 河北保定 071000

收稿日期:2019-10-17 出版日期:2020-04-25 发布日期:2020-04-25
作者简介:杨卫(1979—),男,河北保定人,副主任医师,博士,硕士生导师,主要从事呼吸系统疾病的临床与科研,尤其擅长支气管哮喘方向的研究。 E-mail: hdfyyw@sina.com
基金资助:
保定市科学技术研究与发展计划项目(18ZF193)

Received:2019-10-17 Online:2020-04-25 Published:2020-04-25

摘要/Abstract

摘要： 目的利用哮喘动物模型,研究早期吸入布地奈德治疗后对哮喘动物肺功能、骨桥蛋白、α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)的影响。方法 60只清洁级雄性SD 大鼠随机分成对照组、模型组和治疗组。模型组和治疗组通过腹膜腔注射卵清蛋白和反复雾化吸入卵清蛋白的方式制备动物模型,对照组动物使用生理盐水对照。治疗组早期雾化吸入布地奈德,对照组和模型组雾化吸入生理盐水。观察3组动物肺功能、骨桥蛋白和α-SMA的表达变化。结果与对照组比较,模型组、治疗组出现基本气道阻力增高、气道反应性增高,治疗组基本气道阻力和气道反应性好于模型组。模型组和治疗组骨桥蛋白、气道α-SMA表达较对照组明显升高,治疗组上述指标表达低于模型组。骨桥蛋白的表达与气道阻力呈正相关,与α-SMA表达呈正相关。结论早期吸入布地奈德有利于改善哮喘肺功能、减轻气道重塑,其机制可能与骨桥蛋白的调节有关。

关键词: 哮喘, 布地奈德, 肺功能, 骨桥蛋白, α-平滑肌肌动蛋白

Abstract: Objective To explore the effect of early inhalation of budesonide on lung function, osteopontin and α-SMA in asthmatic animals. Methods Sixty male SD rats were randomly divided into control group, model group and treatment group. The model group and the treatment group were prepared by intraperitoneal injection of ovalbumin and repeated aerosol inhalation of ovalbumin, and the control animals were treated with physiological saline. Changes in lung function, osteopontin, and α-SMA were measured in three groups of animals. Results Compared with the control group, the basic airway resistance and airway responsiveness increased in the model group and the treatment group. The basic airway resistance and airway responsiveness in the treatment group were better than those in the model group. The expression of osteopontin and airway α-SMA in the model group and the treatment group was significantly higher than that in the control group. The expression of the above indicators in the treatment group was lower than that in the model group. The expression of osteopontin was positively correlated with airway resistance, and it was also positively correlated with the expression of α-SMA. Conclusion Early inhalation of budesonide is beneficial to improve lung function and reduce airway remodeling in asthma, and its mechanism may be related to the regulation of osteopontin.

Key words: asthma, budesonide, lung function, osteopontin, α-smooth muscle actin(α-SMA)

中图分类号:

R562.2

杨卫,许晓云,监士宁,王海洋,吴欣. 早期吸入布地奈德对过敏性哮喘模型大鼠肺功能、骨桥蛋白及α-SMA的影响[J]. 医学研究与教育, 2020, 37(2): 31-36.

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早期吸入布地奈德对过敏性哮喘模型大鼠肺功能、骨桥蛋白及α-SMA的影响

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