类风湿关节炎发病不同时期cDC成熟状态研究

doi:10.3969/j.issn.1674-490X.2021.04.001

医学研究与教育 ›› 2021, Vol. 38 ›› Issue (4): 1-7.DOI: 10.3969/j.issn.1674-490X.2021.04.001

• 基础医学 • 下一篇

类风湿关节炎发病不同时期cDC成熟状态研究

滕景芳,陈胜德,梁蕊,徐文斌,孟明

1.河北大学基础医学院, 河北保定 071000;
2.河北省炎性自身免疫性疾病的发病机制及防治重点实验室, 河北保定 071000

收稿日期:2021-07-05 出版日期:2021-08-25 发布日期:2021-08-25
通讯作者: 孟明(1964—),女,辽宁义县人,教授,博士生导师,主要从事自身免疫性疾病、肿瘤免疫及免疫药理学研究。E-mail: mengming127@163.com
作者简介:滕景芳(1997—),女,黑龙江绥化人,在读硕士,主要从事自身免疫性疾病及肿瘤免疫研究。
基金资助:
国家自然科学基金面上项目(81771755)

Received:2021-07-05 Online:2021-08-25 Published:2021-08-25

摘要/Abstract

摘要： 目的采用hGPI325-339和hGPI469-483混合多肽片段构建类风湿关节炎(rheumatoid arthritis,RA)小鼠模型,观测RA小鼠体内经典树突状细胞(conventional dendritic cells,cDC)的频率及成熟状态。方法流式细胞术检测RA模型小鼠发病时脾脏、淋巴结cDC的频率和CD80 、CD86分子的表达情况。结果 RA小鼠脾脏cDC频率在炎症4个时期均显著低于健康对照组;CD80分子的表达在炎症初期、高峰期和缓解期明显高于健康对照组,在炎症进展期明显低于健康对照组;CD86分子的表达在炎症的4个时期均明显高于健康对照组。淋巴结cDC频率在炎症4个时期均显著高于健康对照组;CD80分子的表达在炎症初期明显高于健康对照组,其余3个时期均低于健康对照组;CD86分子的表达在炎症初期、进展期和缓解期明显低于健康对照组,在炎症高峰期高于健康对照组。结论在RA发生中脾脏cDC的抗原提呈作用较之淋巴结可能更为重要;RA中脾脏和淋巴结cDC的成熟度存在先升高后降低的起伏波动现象。

关键词: 类风湿关节炎, cDC, CD80, CD86

Abstract: Objective To establish a mouse model of RA with hGPI325-339 and hGPI469-483 mixed peptides and observe the frequency and mature status of cDC in RA mice.Methods The frequency of cDC and the expression of CD80 and CD86 in spleen and lymph nodes of RA model mice were detected by flow cytometry.Results The frequency of cDC in spleen of RA mice was significantly lower than that of normal control group during the four stages of inflammation.The expression of CD80 was significantly higher than normal control group in the initial stage, peak stage and remission stage of inflammation, and was significantly lower than normal control group in the progressive stage of inflammation.The expression of CD86 was significantly higher than normal control group in all four stages of inflammation.The frequency of cDC in lymph nodes was significantly higher than normal control group in all four stages of inflammation.The expression of CD80 was significantly higher than normal control group at the initial stage of inflammation and lower than normal control group at other three stages.The expression of CD86 was significantly lower than normal control group in the initial, progressive and remission stages of inflammation, and higher than normal control group in the peak of inflammation.Conclusion The antigen presenting role of splenic cDC may be more important than that of lymph nodes in RA.The cDC maturity of spleen and lymph node in RA show a fluctuating phenomenon that increases at first and then decreases.

Key words: rheumatoid arthritis(RA), cDC, CD80, CD86

中图分类号:

R392.8

滕景芳,陈胜德,梁蕊,徐文斌,孟明. 类风湿关节炎发病不同时期cDC成熟状态研究[J]. 医学研究与教育, 2021, 38(4): 1-7.

参考文献

[1] 栗占国, 张奉春, 鲍春德. 类风湿关节炎[M]. 北京: 人民卫生出版社, 2009:205-236.
[2] SCOTT D L, WOLFE F, HUIZINGA T W. Rheumatoid arthritis[J]. Lancet, 2010, 376(9746): 1094-1108. DOI: 10.1016/s0140-6736(10)60826-4.
[3] BOISSIER M C, ASSIER E, FALGARONE G, et al. Shifting the imbalance from Th1/Th2 to Th17/treg: the changing rheumatoid arthritis paradigm[J]. Joint Bone Spine, 2008, 75(4): 373-375. DOI: 10.1016/j.jbspin.2008.04.005.
[4] 张雪娇, 刘嘉琳, 杨飞, 等. 葡萄糖-6-磷酸异构酶混合肽段诱导的DBA/1小鼠类风湿性关节炎模型的建立[J]. 中国病理生理杂志, 2016, 32(3):569-576.
[5] CHEN D, LIU H, WANG Y, et al. Study of the adoptive immunotherapy on rheumatoid arthritis with Thymus-derived invariant natural killer T cells[J]. Int Immunopharmacol, 2019, 67: 427-440. DOI: 10.1016/j.intimp.2018.12.040.
[6] WEHR P, PURVIS H, LAW S C, et al. Dendritic cells, T cells and their interaction in rheumatoid arthritis[J]. Clin Exp Immunol, 2019, 196(1): 12-27. DOI: 10.1111/cei.13256.
[7] GANGULY D, HAAK S, SISIRAK V, et al. The role of dendritic cells in autoimmunity[J]. Nat Rev Immunol, 2013, 13(8): 566-577. DOI: 10.1038/nri3477.
[8] LUTZKY V, HANNAWI S, THOMAS R. Cells of the synovium in rheumatoid arthritis. Dendritic cells[J]. Arthritis Res Ther, 2007, 9(4): 1-12. DOI: 10.1186/ar2200.
[9] YU M B, LANGRIDGE W H R. The function of myeloid dendritic cells in rheumatoid arthritis[J]. Rheumatol Int, 2017, 37(7): 1043-1051. DOI: 10.1007/s00296-017-3671-z.
[10] ZIEGLER-HEITBROCK L, ANCUTA P, CROWE S, et al. Nomenclature of monocytes and dendritic cells in blood[J]. Blood, 2010, 116(16): e74-e80. DOI: 10.1182/blood-2010-02-258558.
[11] IBERG C A, JONES A, HAWIGER D. Dendritic cells as inducers of peripheral tolerance[J]. Trends Immunol, 2017, 38(11): 793-804. DOI: 10.1016/j.it.2017.07.007.
[12] FUNES S C, MANRIQUE DE LARA A, ALTAMIRANO-LAGOS M J, et al. Immune checkpoints and the regulation of tolerogenicity in dendritic cells: Implications for autoimmunity and immunotherapy[J]. Autoimmun Rev, 2019, 18(4): 359-368. DOI: 10.1016/j.autrev.2019.02.006.
[13] SIEGAL F P, KADOWAKI N, SHODELL M, et al. The nature of the principal type 1 interferon-producing cells in human blood[J]. Science, 1999, 284(5421): 1835-1837. DOI: 10.1126/science.284.5421.1835.
[14] BOCKERMANN R, SCHUBERT D, KAMRADT T, et al. Induction of a B-cell-dependent chronic arthritis with glucose-6-phosphate isomerase[J]. Arthritis Res Ther, 2005, 7(6): R1316-R1324. DOI: 10.1186/ar1829.
[15] KAMRADT T, SCHUBERT D. The role and clinical implications of G6PI in experimental models of rheumatoid arthritis[J]. Arthritis Res Ther, 2005, 7(1): 20-28. DOI: 10.1186/ar1476.
[16] SARKAR S, FOX D A. Dendritic cells in rheumatoid arthritis[J]. Front Biosci, 2005, 10: 656-665. DOI: 10.2741/1560.
[17] SHARPE A H, FREEMAN G J. The B7-CD28 superfamily[J]. Nat Rev Immunol, 2002, 2(2): 116-126. DOI: 10.1038/nri727.

类风湿关节炎发病不同时期cDC成熟状态研究

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