Experimental study of total flavonoids in Net Cliffbean affecting the mechanical allodynia in CCI rats

Abstract

Abstract: Objectivev To study the effects of total flavonoids in Net Cliffbean on the mechanical allodynia and glutamate contents in spinal of chronic constriction injury of the sciatic(CCI) rats. Methods 40 rats were randomly divided into 4 groups: control group, sham operation group, CCI group and Net Cliffbean treatment group, with NomralSaline or drugs, once a day, for 4 weeks. Mechanical withdrawal threshold (MWT) was measured as the sign of mechanical allodynia for 3, 7, 10, 14, 21, 28 day. The lumbar segment of the spinal cord was removed for determination of glutamate contents by high pressure liquid chromatography (HPLC) for 14, 28 day. Results Total flavonoids in Net Cliffbean significantly increased the mechanical withdrawal threshold (MWT) compared with model group (P＜0.05 or P＜0.01), and obviously decreased the content of glutamate in the spinal cord (P＜0.01). Conclusion Total flavonoids in Net Cliffbean can relieve the mechanical allodynia in neuropathic pain rats, its mechanism maybe reduced glutamate content in the spinal cord.

Key words: Net Cliffbean, total flavonoids, neuropathic pain, mechanical allodynia, glutamate

CLC Number:

LIU Yangbin, ZHANG Zhihua, CHEN Yayun. Experimental study of total flavonoids in Net Cliffbean affecting the mechanical allodynia in CCI rats[J]. Medical Reserch and Education, 2015, 32(1): 7-11.

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