doi:10.3969/j.issn.1674-490X.2023.02.001

Abstract

Abstract: Objective To study the molecular mechanism and potential therapeutic targets of premature ovarian failure(POF)by screening the differentially expressed genes(DEGs)through bioinformatics analysis. Methods The gene microarrays related with POF were selected from GEO database, the GEO2R online analysis tool was used to screen for DEGs. Target genes were predicting from the mircode database and miRNA-target genes predicting website, network model of gene interaction was constructed by the Cytoscape 3.7.2. GO and KEGG analysis of target genes were performed on DAVID database. Results A total of 1 110 DEGs were screened(P<0.01, |log₂ FC|≥1.0), including 905 up-regulated genes and 205 down-regulated genes, target genes of which mainly associated with metal ion transmembrane transporter activity, DNA and transcription factor binding ability, protein serine/threonine kinase activity were involved in PI3K-Akt、MAPK、Rap1、Ras and Ca²⁺ signal pathways. Conclusion This study indicates that screening for DEGs then analyzing pathways and functions of target genes could help to understand the molecular mechanism of POF, and provide with new therapeutic targets for POF.

Key words: premature ovarian failure, granular cell, bioinformatics analysis, differentially expressed genes, target genes

CLC Number:

R711.75

References

[1] HUANG Y J, ZHU M D, LIU Z, et al. Bone marrow mesenchymal stem cells in premature ovarian failure: mechanisms and prospects[J]. Front Immunol, 2022, 13: 997808. DOI: 10.3389/fimmu.2022.997808.
[2] 艾红云,肖炳坤.补肾中药治疗卵巢早衰的研究进展[J].中医临床研究, 2014, 6(16): 144-146. DOI: 10.3969/j.issn.1674-7860.2014.16.077.
[3] 黄旭春,曹晓静,林楠,等.卵巢早衰中医诊疗指南评价与修订[J].河南中医, 2019, 39(1): 82-86. DOI: 10.16367/j.issn.1003-5028.2019.01.0020.
[4] 黄家语,杜艳芝.长链非编码RNA在卵泡发育及其相关疾病中的研究进展[J].中华生殖与避孕杂志, 2020, 40(11): 958-963. DOI: 10.3760/cma.j.cn101441-20191111-00505.
[5] 陈亚楠,李瑞梅,陈晓丽,等.长链非编码RNA与卵泡发育相关性的研究进展[J].生殖医学杂志, 2020, 29(12): 1677-1681. DOI: 10.3969/j.issn.1004-3845.2020.12.025.
[6] 董书餐,侯碧巍,邹娴,等.细胞自噬对动物卵泡发育调控的研究进展[J].中国畜牧兽医, 2022, 49(11): 4335-4345. DOI: 10.16431/j.cnki.1671-7236.2022.11.024.
[7] 汪鸽,韩延华.早发性卵巢功能不全的颗粒细胞凋亡机制及中医药干预研究进展[J].中医药信息, 2022, 39(2): 83-88. DOI: 10.19656/j.cnki.1002-2406.20220215.
[8] LIU Z T, LI F H, XUE J W, et al. Esculentoside A rescues granulosa cell apoptosis and folliculogenesis in mice with premature ovarian failure[J]. Aging, 2020, 12(17): 16951-16962. DOI: 10.18632/aging.103609.
[9] ZHANG H, QIN F F, LIU A L, et al. Electro-acupuncture attenuates the mice premature ovarian failure via mediating PI3K/AKT/mTOR pathway[J]. Life Sci, 2019, 217: 169-175. DOI: 10.1016/j.lfs.2018.11.059.
[10] YAN F, ZHAO Q, LI Y, et al. The role of oxidative stress in ovarian aging: a review[J]. J Ovarian Res, 2022, 15(1): 100. DOI: 10.1186/s13048-022-01032-x.
[11] SUN J Y, GUO Y, FAN Y H, et al. Decreased expression of IDH1 by chronic unpredictable stress suppresses proliferation and accelerates senescence of granulosa cells through ROS activated MAPK signaling pathways[J]. Free Radic Biol Med, 2021, 169: 122-136. DOI: 10.1016/j.freeradbiomed.2021.04.016.
[12] SAWANT K, CHEN Y J, KOTIAN N, et al. Rap1 GTPase promotes coordinated collective cell migration in vivo[J]. Mol Biol Cell, 2018, 29(22): 2656-2673. DOI: 10.1091/mbc.E17-12-0752.
[13] FAN H Y, LIU Z L, MULLANY L K, et al. Consequences of RAS and MAPK activation in the ovary: the good, the bad and the ugly[J]. Mol Cell Endocrinol, 2012, 356(1/2): 74-79. DOI: 10.1016/j.mce.2011.12.005.
[14] TIWARI M, PRASAD S, SHRIVASTAV T G, et al. Calcium signaling during meiotic cell cycle regulation and apoptosis in mammalian oocytes[J]. J Cell Physiol, 2017, 232(5): 976-981. DOI: 10.1002/jcp.25670.

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 2

Recommended Articles

Metrics

Comments

[1]	. [J]. Medical Reserch and Education, 2009, 26(6): 81-84.
[2]	. [J]. Medical Reserch and Education, 2009, 26(4): 48-49.