Effects of calcium channel blockers on the distribution of blood vessels around the scar after nerve injury

doi:10.3969/j.issn.1674-490X.2015.01.007

Abstract

Abstract: Objective To investigate the correlation between the calcium channel blockers on the distribution of blood vessels scar after sciatic nerve injury in rats. Methods 96 SD rats were divided into 4 group at random, 24 rats in each group. The experimental rats were made sciatic nerve injury model, the experimental group and control group were given respectively a intraperitoneal injection of verapamil and physiological saline(4 mg/kg), in 4, 8 weeks after surgery immunohistochemical staining and vascular perfusion were conducted to observe the scar after injury of vascular distribution. Results In 4, 8 weeks after surgery, the blood vessel numbers were increased and the density of the nerve lesions became higher, the vascular distributions were more uniform in the rats of the experimental group. However, the results of the controls indicated that the blood vessels numbers were decreased, the density of the nerve lesions became lower, the vascular distributions were in the state of disorder. Conclusion Calcium channel blockers can be used to reduce the formation of collagen fibers to soften the scars, and thus improve the distribution of the lesions of blood vessels and promote the blood supply of nerve tissue damage, and make the damaged nerve bundle membrane and blood capillary hyperplasia of outer membrane recovered to accelerate the nerve regeneration and speed up the recovery of neural functions.

Key words: calcium channel blockers, nerve scar, sciatic nerve injury, nerve regeneration, angioarchitecture

CLC Number:

R361

LIU Chong, LIU Xiaofeng, CHEN Taoping, WANG Yunfei, REN Bao, GUO Zhao, XUE Jinwei, LIU Shuangli. Effects of calcium channel blockers on the distribution of blood vessels around the scar after nerve injury[J]. Medical Reserch and Education, 2015, 32(1): 31-35.

References

[1] YANG J Y, HUANG Chunyuan. The effect combined steroid and calcium channel blocker injection on human hypertrophic scars in animal model: a new strategy for the treatment of hypertrophic scars[J]. Dermatol Surg, 2010, 36(12): 1942-1949.
[2] 张玉春, 彭旦明. 瘢痕组织增生微血管构筑研究进展[J]. 亚太传统医药, 2007, 3(10): 29-32.
[3] 郭月超, 王恩波, 赵群, 等. 印度墨汁灌注观察兔股骨头血运[J]. 中国医科大学学报, 2011, 40(4): 371-372.
[4] ATKINS S, SMITH K G, LOESCHERA R, et al. Scarring impedes regeneration at sites of peripheral nerve repair[J]. NeuroReport, 2006, 17(12): 1245-1249.
[5] 薛金伟, 凌丽, 张敏. 钙通道阻滞剂对大鼠周围神经损伤后组织形态学的影响[J]. 中国老年学杂志, 2011, 31(9): 1607-1609.
[6] 薛金伟, 凌丽, 张敏. 钙通道阻滞剂对体外培养神经瘢痕成纤维细胞形态及胶原分泌的影响[J]. 中国老年学杂志, 2011, 31(8): 1385-1387.
[7] 黄如林, 梁杰, 夏琼平, 等. CD34 及 CD105 两种微血管标记物在瘢痕微血管构筑中的比较[J]. 中国组织工程研究与临床修复, 2011, 15(11): 1977-1980.
[8] MUSTOE T A, COOTER R D, GOLD M H, et al. International clinical recommendations on scar management[J]. Plast Reconstr Surg, 2002, 110(2): 560-571.
[9] 李小静, 孟刚, 宁金龙, 等. CD34 组化法在瘢痕微血管构筑中的应用研究[J]. 中国烧伤创伤杂志, 2000, 12(1): 13-15.
[10] 向军, 王西樵, 刘英开, 等. 烧伤后瘢痕组织中成纤维细胞 ET-1 表达及血管新生和胶原分布研究[J]. 上海交通大学学报: 医学版, 2010, 30(7): 839-842.
[11] ZHU K Q, ENGRAV L H. Changes in VEGF and nitricoxide after deep dermal injury in the female, red Duroc pig-further similarities between female, Duroc scar and human hypertrophic scar[J]. Burns, 2005, 31(1): 5.
[12] 童建斌, 曾乐平, 陈旦, 等. 明胶墨汁灌注展示大鼠视网膜血管的方法[J]. 第三军医大学学报, 2007, 29(21): 2031-2033.
[13] MAAS R, SCHWEDHELM E, ALBSMEIER J, et al. The pathophysiology of erectile dysfunction related to endothelial dysfunction and mediators of vascular function[J]. Vasc Med, 2002, 7(3): 213-225.
[14] SHARIF M R, SHAANANI A, MAHMOUDI H, et al. Association of the serum vascular endothelial growth factor levels with benign prostate hyperplasia and prostate malignancies[J]. Nephro Urol Mon, 2014, 6(3): e14778.
[15] 刘明阁, 于庆凯. 肝细胞癌肿瘤血管生成及其临床病理意义[J]. 河南医学研究, 2014, 23(10): 4-6.
[16] 周武. 内皮祖细胞的生物学性状及其治疗作用的研究进展[J]. 东南大学学报: 医学版, 2014, 33(6): 783-787.
[17] HOLLINGSWORTH H C, KOHN E C, STEINBERG S M, et al. Tumor angiogenesis in advanced stage ovarian carcinoma[J]. Am J Pathol, 1995, 147(1): 33-41.
[18] ALSTER T S, HANDRICK C. Laser treatment of hypertrophic scars, keloids, and striae[J]. Semin Cutan Med Surg, 2000, 19(4): 287-292.