Abstract: Objective Survivin gene is the strongest apoptosis-inhibition factor among inhibitor of apoptosis protein (IAP), and purification of fusion protein of functional domains (baculovirus IAP repeat, BIR) may provide the foundation for interaction research between the gene and related other genes. Methods BIR sequence, after ampliifcation with PCR and validation by Escherichia coli DH5αthrough pGEX4T-2 vector, was transformed into E. coli BL21. Fusion protein of BIR was induced by IPTG and puriifed by Glutathione Agarose Affinity Chromatography. Results The amplified fragment BIR was about 276bp, purified fusion protein molecular weight of approximately 37ku. Conclusion PCR results are consistent with the expected fragment size, recombinant plasmids after 2h after IPTG induction, the cell in the fusion protein increase signiifcantly, which provide basis for further research on Survivin gene function and its interacting protein.

Key words: Survivin, pGEX4T-2, IPTG induction, puriifcation