doi:10.3969/j.issn.1674-490X.2021.06.001

Abstract

Abstract: Objective To investigative the effect of resolvin D1(RvD1)in the rat's liver IRI and the possible mechanism. Methods SD rats were divided into 4 groups randomly: Sham group, IR group, RvD1 group(IR+RvD1)and ZnPP group(IR+ RvD1+ZnPP). The levels of ALT and AST in the serum were detected with biochemical analyzer. MPO in the rat's liver were detected with MPO kit. The changes of liver cell were observed with H-E staining. The mRNA level of ICAM-1 in rat's liver were tested with quantitative real-time PCR(qRT-PCR). Results In RvD1 group, the levels of ALT(P<0.05)and AST(P<0.01)were decreased significantly. The swellings and necrosis in the rat liver cells in RvD1 group were observed to alleviate through H-E staining. Compared with IR group, the level of MPO in RvD1 group was reduced by MPO kit. And the mRNA level of ICAM-1 in RvD1 group was measured to decrease by qRT-PCR. Conclusion RvD1 attenuates the IRI in rat's liver by reducing the level of MPO and ICAM-1 and the protective role is reversed by the inhibitor of HO-1, ZnPP.

Key words: resolvin D1, ischemia reperfusion, myeloperoxidase, intercellular adhesion molecule-1, liver

CLC Number:

R575

References

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