doi:10.3969/j.issn.1674-490X.2021.06.003

Abstract

Abstract: Objective To investigate the effects of DNA fragmentation index(DFI)on the clinical pregnancy rate of frozen-thawed embryo transfer(FET)after intracytoplasmic sperm injection(ICSI). Methods The general data of 87 couples who received ICSI for assisted reproductive technology were retrospectively analyzed. Female infertility, chromosomal structural abnormalities of both couples and male Y chromosome microdeletion were excluded. Testicular volume(Ultrasound Doppler)assessment, serum FSH and TT tests, routine semen analysis, percentage of sperm DNA fragments, and clinical pregnancy rates were statistically analyzed. Results There were no significant differences in female age, male age, infertility years, estradiol(E2)on FET day, intrauterine thickness on FET day. There were significant differences between DFI≤25% and DFI>25% group on fertilization rate(80.92% vs 70.51%), 2PN formation rate(85.37% vs 71.71%), cleavage rate(96.34% vs 88.05%), high quality embryo rate(23.56% vs 11.30%), blastocyst formation rate(31.78% vs 14.14%). The clinical pregnancy rate(45.24%)in DFI ≤25% group was higher than that in DFI>25% group(20.00%)and the difference was statistically significant(χ²=6.340, P=0.006). Conclusion Studies show that sperm DFI is closely related to pregnancy outcome, and the unusually high sperm DFI can affect the clinical pregnancy rate adversely.

Key words: sperm function, sperm quality, pregnancy outcome sperm, DNA fragmentation index

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References

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