doi:10.3969/j.issn.1674-490X.2023.01.001

Abstract

Abstract: Objective To investigate the effect of ginsenoside Rg1 on the proliferation of adriamycin(ADR)-resistant K562/ADR cells in human chronic myeloid leukemia(CML)and its reversal of drug resistance in K562/ADR cells. Methods CCK-8 assay was used to detect the effects of different concentrations of ginsenoside Rg1 on the proliferation of K562/ADR cells. CCK-8 assay was used to detect the effects of ginsenoside Rg1 combined with ADR on the proliferation of K562/ADR cells, and the half maximal inhibitory concentration(IC₅₀)and reversal fold were calculated. Colony formation assay was used to detect the effects of ginsenoside Rg1 combined with ADR on the colony formation ability of K562/ADR cells. Flow cytometry was used to detect the effects of ginsenoside Rg1 combined with ADR on the cell cycle of K562/ADR cells. Results Compared with the control group, the proliferation inhibition rate of K562/ADR cells treated with 120 μg/ml ginsenoside Rg1 for 48 h was significantly increased(P<0.001). Compared with the ADR alone group, the IC₅₀ value of ginsenoside Rg1 combined with ADR for 48 h decreased significantly in K562/ADR cells(P<0.05), and the fold reversal of ginsenoside Rg1 resistance to K562/ADR cells was 2.34. Compared with the control group and ADR or ginsenoside Rg1 alone group, ginsenoside Rg1 combined with ADR could significantly inhibit K562/ADR cell colony formation(P<0.05). Compared with the ADR or ginsenoside Rg1 alone group, ginsenoside Rg1 combined with ADR could arrest the cell cycle in G₀/G₁ phase. Conclusion Ginsenoside Rg1 combined with ADR can significantly inhibit the proliferation of K562/ADR cells and reverse the resistance of K562/ADR cells to ADR, which may be related to the cell cycle arrest in G₀/G₁ phase.

Key words: ginsenoside Rg1, adriamycin, K562/ADR cells, cell cycle, drug resistance

CLC Number:

R733.72

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