α-玉米赤霉醇对大鼠成骨细胞IL-6表达及分泌的影响

doi:10.3969/j.issn.1674-490X.2014.01.001

摘要/Abstract

摘要： 目的：探讨α-玉米赤霉醇对体外培养的大鼠成骨细胞IL-6表达及分泌的影响。方法体外培养原代SD新生大鼠颅骨成骨细胞，形态学观察、碱性磷酸酶(ALP)染色、I型胶原免疫荧光染色进行成骨细胞鉴定，分别以雌激素及不同浓度的α-玉米赤霉醇作用于成骨细胞，采用RT-PCR法检测IL-6 mRNA的表达，ELISA法检测成骨细胞培养上清中IL-6的含量。结果10-10~10-5mol/Lα-玉米赤霉醇能够抑制成骨细胞IL-6 mRNA的表达及其蛋白分泌，其中以10-6 mol/L、10-7mol/L的α-玉米赤霉醇作用显著。结论α-玉米赤霉醇能够抑制成骨细胞细胞因子IL-6 mRNA及蛋白的表达，这可能是α-玉米赤霉醇防治绝经后骨质疏松症的机制之一。

关键词: α-玉米赤霉醇, 成骨细胞, 白细胞介素6, 绝经后骨质疏松症

Abstract: Objective To investigate the effects ofα-zeranol on the IL-6 expression and secretion in rat osteoblasts. Methods Osteoblasts were isolated from the skull of SD neonatal rats and identified by morphological observation, ALP staining, and collagen I immunolfuorescence staining. 17β-estradiol andα-zeranol with different concentration were administrated. The expression of IL-6 mRNA in the osteoblasts was detected by RT-PCR and the level of IL-6 in culture supernatant of osteoblasts was measured by ELISA. Results α-zeranol with 10-10~10-5 mol/L concentrations could inhibit the expression and secretion of IL-6 in osteoblasts, among which the concentrations of 10-6 and 10-7 mol/L had obvious effects. Conclusionα-zeranol can inhibit the expressions of IL-6 mRNA and protein in osteoblasts, it may play an important role in the prevention of postmenopausal osteoporosis.

Key words: α-zeranol, osteoblasts, IL-6, postmenopausal osteoporosis

中图分类号:

R977

段斐,郝华超,薛娟,李少春,刘未华,谷雨,孙晓芳. α-玉米赤霉醇对大鼠成骨细胞IL-6表达及分泌的影响[J]. 医学研究与教育, 2014, 31(1): 1-7.

DUAN Fei,HAO Huachao,XUE Juan,LI Shaochun,LIU Weihua,GU Yu,SUN Xiaofang. Effects ofα-zeranol on the IL-6 expression and secretion in rat osteoblasts[J]. Medical Reserch and Education, 2014, 31(1): 1-7.

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