非梗阻性不育患者精子膜超微结构研究

doi:10.3969/j.issn.1674-490X.2014.01.002

医学研究与教育 ›› 2014, Vol. 31 ›› Issue (1): 8-12.DOI: 10.3969/j.issn.1674-490X.2014.01.002

非梗阻性不育患者精子膜超微结构研究

王然¹,殷会莹¹,郭明申²,刘淑卓³,葛少钦¹

1.河北大学基础医学院,河北保定,071000
2.河北大学生命科学学院,河北保定,071000
3.河北大学附属医院,河北保定,071000

出版日期:2014-02-25 发布日期:2014-02-25
基金资助:
河北省自然科学基金项目(H2013201259)；河北省卫生厅医学科学研究计划项目(2009173)；河北省计生委科研计划项目(2009-B16)；河北省教育厅自然科学研究计划项目(2009105)；河北大学医学学科建设项目(2012A1004)；河北大学博士基金项目(2007B06)；河北省2013年留学人员科技活动项目；河北大学创业训练项目

Study on sperm membrane ultrastructure of non-abstructive infertile male

WANG Ran¹,YIN Huiying¹,GUO Mingshen²,LIU Shuzhuo³,GE Shaoqin¹

Online:2014-02-25 Published:2014-02-25

摘要/Abstract

摘要： 目的：研究非梗阻性不育患者精子膜超微结构的改变，探讨非梗阻性不育患者精子膜超微结构缺陷对男性不育的影响。方法利用透射电镜对临床非梗阻性不育患者新鲜精液标本中精子膜的超微结构进行观察。结果在电镜下，发现该不育男性患者精子多存在质膜、顶体外膜和/或顶体内膜等畸变，膜结构呈多形态超微结构异常的现象，此外，精子核、颈部和线粒体、微管等超微结构也存在病理性改变。结论精子膜超微结构畸变是非梗阻性男性不育原因之一。

关键词: 非梗阻性不育, 精子膜, 超微结构

Abstract: Objective To explore the inlfuence of sperm membrane ultrastructure damage on the non-obstructive infertile male. Methods After embedding the fresh semen from one case of infertile male, using transmission electron microscopy (TEM) to observe the sperm plasma membrane, outer acrosomal membrane , inner acrosomal membrane etc. Results Under the electron microscope, the defects of plasma membrane, outer acrosomal membrane and inner acrosomal membrane in the semen sperm of this infertile man were examined, with some of other abnomal spermultrastructures observed, such as defects in the sperm acrosome, nucleus, neck, mitochondrial and microtubules. Conclusion The non-obstructive male infertility is closely related to the abnormality of sperm membrane ultrastructure.

Key words: non-obstructive infertility, sperm membrane, ultrastructure

中图分类号:

王然,殷会莹,郭明申,刘淑卓,葛少钦. 非梗阻性不育患者精子膜超微结构研究[J]. 医学研究与教育, 2014, 31(1): 8-12.

WANG Ran,YIN Huiying,GUO Mingshen,LIU Shuzhuo,GE Shaoqin. Study on sperm membrane ultrastructure of non-abstructive infertile male[J]. Medical Reserch and Education, 2014, 31(1): 8-12.

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非梗阻性不育患者精子膜超微结构研究

Study on sperm membrane ultrastructure of non-abstructive infertile male

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