化积健脾汤对肥胖导致雄性小鼠生殖功能损伤的治疗效果

doi:10.3969/j.issn.1674-490X.2019.01.008

医学研究与教育 ›› 2019, Vol. 36 ›› Issue (1): 37-45.DOI: 10.3969/j.issn.1674-490X.2019.01.008

化积健脾汤对肥胖导致雄性小鼠生殖功能损伤的治疗效果

陈兰玉¹,吴银玲¹,谷钇蔚¹,陈彬¹,李孟鑫¹,陶美慧¹,朱梦梦¹,葛少钦^1,2

1.河北大学医学院, 河北保定 071000;
2.河北大学中医学院, 河北保定 071000

收稿日期:2018-11-27 出版日期:2019-02-25 发布日期:2019-02-25
通讯作者: 葛少钦( 1967—),男,河北深州人,教授,博士,主要从事生殖医学研究。E-mail: gesq67@163.com
作者简介:陈兰玉(1991—),女,山东临沂人,在读硕士。
基金资助:
河北省自然科学基金(H2018201218);河北大学创新资助项目(hbu2018ss77)

Received:2018-11-27 Online:2019-02-25 Published:2019-02-25

摘要/Abstract

摘要： 目的探讨“化积健脾汤”对脾虚湿阻型肥胖雄性小鼠体重以及其生殖功能降低的治疗效果。方法制造肥胖动物模型,给予中药和西药治疗,取材,分析体重,从睾丸和附睾湿量及其系数,精子密度、活率及活力等精液参数,睾丸和附睾组织形态学等角度评估肥胖对生殖功能的影响及中药的治疗效果。结果模型空白组小鼠的体重和附睾湿重明显高于正常组和给药组。相较于模型空白组的睾丸湿量及其系数、附睾系数和精液参数下降等问题,睾丸和附睾结构松散脱落的现象,中药实验组和奥利司他对照组均有改善,但在提高睾丸湿重和改善精液参数方面,中药效果尤为突出。结论“化积健脾汤”可有效抑制脾虚湿阻型肥胖小鼠体重的快速增长,对肥胖诱发的生殖损伤有一定的治疗作用。

关键词: 化积健脾汤, 肥胖雄性小鼠, 生殖功能损伤, 治疗效果

Abstract: Objective To explore the effect of Huji Jianpi Decoction on weight inhibition and reproductive function injury caused by obesity in male mice with spleen deficiency dampness resistance. Methods An animal model of obesity was established then given treatment with traditional Chinese medicine and western medicine, the body weight was examined. The effects of obesity on reproductive function were evaluated from the aspects mentioned below, weight and histomorphology of testicular and epididymal, and semen parameters such as sperm concentration, sperm viability and sperm motility. The therapeutic effect of Chinese medicine were also measured by the above parameters. Results The weight and wet weight of epididymis in model blank mices were obviously higher than that of normal group and drug group. Compared with the model group, the problem including the testicular moisture and its coefficient, epididymal coefficient and semen parameters decreased, and the phenomenon containing the loose and detached structures of testicle and epididymis were improved in both the Chinese medicine experimental group and orlistat control group. However, the effects of traditional Chinese medicine were outstanding in improving testicular moisture weight and semen parameters. Conclusion Huaji Jianpi Decoction can effectively inhibit the rapid growth of weight and has a certain therapeutic effect on obesity-induced reproductive damage in obese mice.

Key words: Huaji Jianpi Decoction, obese male mice, reproductive impairment, treatment effect

中图分类号:

R242

陈兰玉,吴银玲,谷钇蔚,陈彬,李孟鑫,陶美慧,朱梦梦,葛少钦. 化积健脾汤对肥胖导致雄性小鼠生殖功能损伤的治疗效果[J]. 医学研究与教育, 2019, 36(1): 37-45.

参考文献

[1] BAKOS H W, MITCHELL M, SETCHELL B P, et al. The effect of paternal diet-induced obesity on sperm function and fertilization in a mouse model.[J]. Int J Androl, 2011, 34(5pt1):402-410. DOI: 10.1111/j.1365-2605.2010.01092.x.
[2] MALCHER A, ROZWADOWSKA N, STOKOWY T, et al. The gene expression analysis of paracrine/autocrine factors in patients with spermatogenetic failure compared with normal spermatogenesis[J]. Am J Reprod Immuno, 2013, 70(6):522-528. DOI: 10.1111/aji.12149.
[3] MARTINI A C, MOLINA R I, RUIZ R D, et al. Obesity and male fertility[J]. Rev Fac Cien Med Univ Nac Cordoba, 2012, 69(2):102-110. DOI: 10.1111/andr.270.
[4] YUAN M, HUANG G, LI J, et al. Hyperleptinemia directly affects testicular maturation at different sexual stages in mice, and suppressor of cytokine signaling 3 is involved in this process[J]. Reprod Biol Endoc, 2014, 12(1):15-15. DOI: 10.1186/1477-7827-12-15.
[5] PALMER N O, BAKOS H W, FULLSTON T, et al. Impact of obesity on male fertility, sperm function and molecular composition[J]. Spermatogenesis, 2012, 2(4):253-263. DOI: 10.4161/spmg.21362.
[6] HAMMOUD A O, WILDE N, GIBSON M, et al. Male obesity and alteration in sperm parameters[J]. Fertil Steril, 2008, 90(6):2222-2225. DOI: 10.1016/j.fertnstert.2007.10.011.
[7] GON K I, CUSICK M E, VALLE D, et al. The human disease network[J]. P Nati Acad Sci USA, 2007, 104(21):8685-8690. DOI: 10.1073/pnas.0701361104.
[8] 董红彦, 杜武勋, 张富庚,等. 网络药理学及其在中医药领域的研究进展[J]. 中医药导报, 2016(18):67-70. DOI: 10.13862/j.cnki.cn43-1446/r.2016.18.0.
[9] OZDEN C, OZDAL O L, URGANCIOGLU G, et al. The correlation between metabolic syndrome and prostatic growth in patients with benign prostatic hyperplasia[J]. Eur Urol, 2007, 51(1):199-206. DOI: 10.1016/j.eururo.2006.05.040.
[10] CHEN M M, LAN X X, LI C Y, et al. Diet-induced obesity increases the apoptosis of testicular spermatogenic cells in pubertal male rats[J]. Int J Androl, 2011, 17(4):342. DOI: 10.1007/s10570-010-9464-0.
[11] KIM N, NAKAMURA H, MASAKI H, et al. Effect of lipid metabolism on male fertility[J]. Biochem Bioph Res, 2017, 485(3): 686-692. DOI: 10.1016/j.bbrc.2017.02.103.
[12] CRAIG J R, JENKINS T G, CARRELL D T, et al. Obesity, male infertility, and the sperm epigenome[J]. Fertil Steril, 2017, 107(4): 848-859. DOI: 10.1016/j.fertnstert.2017.02.115.
[13] SERMONDADE N, FAURE C, FEZEU L, et al. BMI in relation to sperm count[J]. Hum Reprod Update, 2013, 19(3):221. DOI: 10.1093/humupd/dms050.
[14] WANG H, ZHAO R, GUO C, et al. Knockout of BRD7 results in impaired spermatogenesis and male infertility[J]. Sci Rep, 2016, 6:21776. DOI: 10.1038/srep21776.
[15] BHAT G K, SEA T L, OLATINWO M O, et al. Influence of a leptin deficiency on testicular morphology, germ cell apoptosis, and expression levels of apoptosis‐related genes in the mouse[J]. J androl, 2006, 27(2): 302-310. DOI: 10.2164/jandrol.05133.
[16] ENGIN-USTUN Y, Y lMAZ N, AKGUN N, et al. Body Mass Index Effects Kruger's Criteria in Infertile Men[J]. Int J Fertil Steril, 2018, 11(4):258-262. DOI: 10.22074/ijfs.2018.4888.
[17] LIU H, LIU X, WANG L, et al. Brown adipose tissue transplantation ameliorates male fertility impairment caused by diet-induced obesity[J]. Obes Res Clin Prac, 2016, 11(2):198-205. DOI: 10.1016/j.orcp.2016.06.001.
[18] TENOVER J L. 老年男性的睾酮替代疗法[J]. 中华男科学杂志, 2001, 7(3):141-146. DOI: 10.13263/j.cnki.nja.2001.03.001.
[19] SAKKAS D, SELI E, BIZZARO D, et al. Abnormal spermatozoa in the ejaculate: abortive apoptosis and faulty nuclear remodelling during spermatogenesis[J]. Reproductive Biomedicine Online, 2003, 7(4):428-432.DOI: 10.1016/S1472-6483(10)61886-X.
[20] SUKHOTNIK I, NATIV O, ROITBURT A, et al. Methotrexate induces germ cell apoptosis and impairs spermatogenesis in a rat[J]. Pediatr Surg Int, 2013, 29(2):179-184.DOI: 10.1007/s00383-012-3197-0.

化积健脾汤对肥胖导致雄性小鼠生殖功能损伤的治疗效果

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 6

编辑推荐

Metrics

本文评价

[1]	杨光福,冯鑫利,赵建喜. 天龙通经活络汤治疗脑出血疗效观察[J]. 医学研究与教育, 2010, 27(6): 61-63.
[2]	范新发,赵秀君,宋红莉,庄英军,赵东东,王志丹,安洪泽. 心脉通1号对冠状动脉内支架植入后再狭窄作用的研究[J]. 医学研究与教育, 2010, 27(6): 56-60,63.
[3]	张敏,郭淑琴,任慧雅,刘竞芳. 化浊导滞、活血化瘀法治疗肥胖型糖尿病眼底出血的临床研究[J]. 医学研究与教育, 2010, 27(5): 52-54,60.
[4]	刘竞芳,郭淑琴,任慧雅,张敏. 化浊导滞、活血化瘀法治疗肥胖型糖尿病性脂肪肝临床观察[J]. 医学研究与教育, 2010, 27(5): 55-57.
[5]	石宇,武变瑛. 复方青黛贴片治疗口腔溃疡62例临床观察[J]. 医学研究与教育, 2010, 27(5): 58-60.
[6]	冯鑫利,杨光福. 中西医结合治疗脑出血研究现状与进展[J]. 医学研究与教育, 2010, 27(2): 86-88.