IFN-α2b对HEL细胞PD-1/PD-L1信号通路的作用及其机制研究

doi:10.3969/j.issn.1674-490X.2022.03.001

医学研究与教育 ›› 2022, Vol. 39 ›› Issue (3): 1-8.DOI: 10.3969/j.issn.1674-490X.2022.03.001

• 基础医学 • 下一篇

IFN-α2b对HEL细胞PD-1/PD-L1信号通路的作用及其机制研究

齐林¹,成志勇²,张丽军¹,王梦²,王宝艳²

1.承德医学院研究生院, 河北承德 067000;
2.保定市第一医院血液内科, 河北保定 071000

收稿日期:2022-03-10 出版日期:2022-06-25 发布日期:2022-06-25
通讯作者: 成志勇(1976—),男,河北保定人,主任医师,博士,硕士生导师,主要从事恶性血液病靶向治疗。E-mail: dzczy@sohu.com
作者简介:齐林(1989—),男,河北石家庄人,主治医师,在读硕士,主要从事恶性血液病靶向治疗。 E-mail: qilinlwzy@163.com
基金资助:
河北省重点研发计划项目(223777105D)

The effect of interferon-α2b on PD-1/PD-L1 signaling pathway of HEL cells

QI Lin¹,CHENG Zhiyong²,ZHANG Lijun¹,WANG Meng²,WANG Baoyan²

1.Graduate School, Chengde Medical University, Chengde 067000, China; 2.Department of Hematology, No.1 Hospital of Baoding, Baoding 071000, China

Received:2022-03-10 Online:2022-06-25 Published:2022-06-25

摘要/Abstract

摘要： 目的研究IFN-α2b对JAK2 V617F阳性人红白血病HEL细胞增殖、凋亡的影响及对程序性死亡受体-1(PD-1)与程序性死亡配体-1(PD-L1)的影响。方法不同浓度IFN-α2b作用于人红白血病HEL细胞系,CCK-8检测细胞活力,Caspase-3/7活性检测试剂盒检测Caspase-3/7活性。荧光定量PCR检测JAK2、PD-1及PD-L1 mRNA表达水平,流式细胞术检测不同浓度IFN-α2b作用HEL后PD-1、PD-L1蛋白表达。结果不同浓度IFN-α2b能够剂量及时间依赖性抑制HEL细胞增殖。IFN-α2b能够剂量及时间依赖性增加HEL细胞Caspase-3/7活性;IFN-α2b能够剂量依赖性降低JAK2 V617F突变量,同时能够抑制HEL细胞PD-1 mRNA及蛋白表达水平,轻度上调PD-L1表达。结论 IFN-α2b可能通过抑制HEL细胞增殖,并参与调控细胞PD-1/PD-L1表达。

关键词: 干扰素, JAK2 V617F突变, HEL细胞, 程序性死亡受体-1, 程序性死亡配体-1

Abstract: Objective To study the effects of interferon-α2b(IFN-α2b)on proliferation and apoptosis and the influence with programmed death-1(PD-1)and programmed death ligand-1(PD-L1)of JAK2 V617F positive human erythroleukemia HEL cells. Methods Different concentrations of IFN-α2b were applied to human erythroleukemia HEL cell lines. CCK-8 was used to detect cell viability and caspase-3/7 activity was detected by caspase-3/7 activity assay kit. The expression levels of JAK2, PD-1 and PD-L1 mRNA were detected by fluorescence quantitative PCR. Flow cytometry was used to detect the effects of different concentrations of interferon-α2b on the expression of PD-1 and PD-L1 protein in HEL. Results Different concentrations of IFN-α2b could inhibit HEL cell proliferation in time and dose-dependent manner. IFN-α2b could increase caspase-3/7 activity of HEL cells in a dose and time-dependent manner; IFN-α2b could reduce JAK2 V617F mutation in a dose-dependent manner,inhibit the expression of PD-1 mRNA and protein, and slightly increase the expression of PD-L1.Conclusion IFN -α2b may inhibit HEL proliferation and participate in the regulation of PD-1/PD-L1 expression.

Key words: interferon alpha, JAK2 V617F mutation, HEL cells, programmed death-1, programmed death ligand-1

中图分类号:

R733.3

齐林,成志勇,张丽军,王梦,王宝艳. IFN-α2b对HEL细胞PD-1/PD-L1信号通路的作用及其机制研究[J]. 医学研究与教育, 2022, 39(3): 1-8.

QI Lin,CHENG Zhiyong,ZHANG Lijun,WANG Meng,WANG Baoyan. The effect of interferon-α2b on PD-1/PD-L1 signaling pathway of HEL cells[J]. Journal of Hebei Medical College for Continuing Education, 2022, 39(3): 1-8.

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IFN-α2b对HEL细胞PD-1/PD-L1信号通路的作用及其机制研究

The effect of interferon-α2b on PD-1/PD-L1 signaling pathway of HEL cells

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