移植骨髓间充质干细胞对帕金森大鼠旋转行为的影响

医学研究与教育 ›› 2012, Vol. 29 ›› Issue (4): 1-5.

• 基础研究 • 下一篇

移植骨髓间充质干细胞对帕金森大鼠旋转行为的影响

张平,李晓芳,李靖华,王鹏,代瑞廷

河北大学附属医院,河北保定 071000

收稿日期:2016-10-09 修回日期:2016-10-09 出版日期:2012-08-25 发布日期:2012-08-25

Received:2016-10-09 Revised:2016-10-09 Online:2012-08-25 Published:2012-08-25

摘要/Abstract

摘要： 目的研究经侧脑室移植骨髓间充质干细胞(mesenchymal stem cells,MSCs )对帕金森(Parkinson’s disease,PD)大鼠行为能力及中脑黑质TH表达的影响.方法雄性SD大鼠36只,随机分为假手术组(Sham组),PD模型组(PD组), MSCs移植组(MSCs组).采用6-OHDA制备PD大鼠模型,经侧脑室移植骨髓MSCs,术后2周、4周、8周,腹腔注射阿朴吗啡诱导PD大鼠旋转行为,采用Western blot方法测定中脑黑质TH蛋白的表达.结果移植术后2周、4周、8周,MSCs组与PD组比较,旋转次数明显减少(P<0.05),移植细胞干预PD模型8周后中脑黑质TH表达,MSCs组较PD组明显增高(P<0.05).结论经侧脑室移植骨髓MSCs通过提高中脑黑质TH的活性,改善PD大鼠的行为能力.

中图分类号:

R329.3

张平,李晓芳,李靖华,王鹏,代瑞廷. 移植骨髓间充质干细胞对帕金森大鼠旋转行为的影响[J]. 医学研究与教育, 2012, 29(4): 1-5.

参考文献

[1] Ted M. Dawson, Valina L. Dawson. Molecular Pathways of Neurodegeneration in Parkinson's Disease [J]. Science 2003, 5646(5646).
[2] PERIER C, BOVE J, WU D C. Two molecular pathways initiate mitochondria-dependent dopaminergic neurodegeneration in experimental Parkinson's disease [J]. Proceedings of the National Academy of Sciences(USA) 2007, 104(19).
[3] GEORGE PAXINOS, CHARLES WATSON, 诸葛启钏. 大鼠脑立体定位图谱 [M]. 北京:人民卫生出版社 2005.
[4] AGGARWAL S, PITTENGER M F. Human mesenchymal stem cells modulate allogeneic immune cell responses [J]. Blood 2005, 105(04).
[5] BERNARDO M E, LOCATELLI F, FIBBE W E. Mesenchymal stromal cells [J]. Annals of the New York Academy of Sciences 2009, 1176.
[6] Kassis I, Grigoriadis N, Gowda-Kurkalli B, Mizrachi-Kol R, Ben-Hur T, Slavin S, Abramsky O, Karussis D. Neuroprotection and immunomodulation with mesenchymal stem cells in chronic experimental autoimmune encephalomyelitis. [J]. Archives of Neurology 2008, 6(6).
[7] Alain Chapel, Jean Marc Bertho, Morad Bensidhoum, Loic Fouillard, Randell G. Young, Johanna Frick, Christelle Demarquay, Frederique Cuvelier, Emilie Mathieu, Francois Trompier. Mesenchymal stem cells home to injured tissues when co-infused with hematopoietic cells to treat a radiation-induced multi-organ failure syndrome [J]. The journal of gene medicine 2003, 12(12).
[8] Zappia E, Casazza S, Pedemonte E, Benvenuto F, Bonanni I, Gerdoni E, Giunti D, Ceravolo A, Cazzanti F, Frassoni F, Mancardi G, Uccelli A. Mesenchymal stem cells ameliorate experimental autoimmune encephalomyelitis inducing T-cell anergy. [J]. Blood 2005, 5(5).
[9] Liu ZJ, Zhuge Y, Velazquez OC. Trafficking and differentiation of mesenchymal stem cells. [J]. Journal of Cellular Biochemistry 2009, 6(6).
[10] Liu ZJ, Zhuge Y, Velazquez OC. Trafficking and differentiation of mesenchymal stem cells. [J]. Journal of Cellular Biochemistry 2009, 6(6).
[11] MEIRELLES LDA S, NARDI N B. Methodology, biology and clinical applications of mesenchymal stem cells [J]. Frontiers in Bioscience 2009, 14.
[12] QU R, LI Y, GAO Q. Neurotrophic and growth factor gene expression profiling of mouse bone marrow stromal cells induced by ischemic brain extracts [J]. Neuropathology:Official Journal of the Japanese Society of Neuropathology 2007, 27(04).
[13] Wakabayashi K, Nagai A, Sheikh AM, Shiota Y, Narantuya D, Watanabe T, Masuda J, Kobayashi S, Kim SU, Yamaguchi S. Transplantation of human mesenchymal stem cells promotes functional improvement and increased expression of neurotrophic factors in a rat focal cerebral ischemia model. [J]. Journal of Neuroscience Research 2010, 5(5).
[14] ZHONG Chi, QIN Zhen, ZHONG Chunjiu. Neuroprotective effects of bone marrow stromal cells on rat organotypic hippocampal slice culture model of cerebral isechemia [J]. Neuroscience Letters 2003, 342(l/2).
[15] Chen J, Li Y, Wang L, Zhang Z, Lu D, Lu M, Chopp M. Therapeutic benefit of intravenous administration of bone marrow stromal cells after cerebral ischemia in rats. [J]. Stroke: A Journal of Cerebral Circulation 2001, 4(4).

移植骨髓间充质干细胞对帕金森大鼠旋转行为的影响

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 3

编辑推荐

Metrics

本文评价

[1]	张庆云,李佳林,郑培,李艳霞,车雨轩,董晓东. 大脑神经递质多巴胺检测方法研究[J]. 医学研究与教育, 2014, 31(1): 87-90.
[2]	张庆云,董晓东,郝媛,陈玉敏. 帕金森病发病机制研究[J]. 医学研究与教育, 2013, 30(3): 85-88,99.
[3]	张庆云,杨慧,董晓东. 铁在帕金森病发病机制中的作用[J]. 医学研究与教育, 2012, 29(4): 55-58.