布鲁菌S2感染后死亡的巨噬细胞启动T细胞应答的作用

doi:10.3969/j.issn.1674-490X.2013.06.001

医学研究与教育 ›› 2013, Vol. 30 ›› Issue (6): 1-4,34.DOI: 10.3969/j.issn.1674-490X.2013.06.001

• 基础研究 • 下一篇

布鲁菌S2感染后死亡的巨噬细胞启动T细胞应答的作用

冯娜¹,王晓曼²,王梦³,任亚莉²,马宁²,曹志然²

1.河北大学基础医学院，河北保定 071000; 河北大学附属医院，河北保定 071000
2.河北大学基础医学院,河北保定,071000
3.河北大学护理学院,河北保定,071000

出版日期:2013-12-25 发布日期:2013-12-25
基金资助:
河北省自然科学基金资助项目

Studies on the effects of dead macrophage infected by Brucella suis S2 to prime T cell response

FENG Na¹,WANG XiaoMan²,WANG Meng³,REN Yali²,MA Ning²,CAO Zhiran²

Online:2013-12-25 Published:2013-12-25

摘要/Abstract

摘要： 目的：研究布鲁菌S2感染死亡的巨噬细胞在启动细胞免疫应答中的作用。方法以布鲁菌S2株体外感染小鼠腹腔巨噬细胞，并以未感染的正常细胞作为对照，感染后1 h用不含血清的RPMI 1640培养基饥饿培养5 d，待细胞完全死亡后与骨髓源性树突状细胞共培养24 h后，ELISA 法检测细胞上清中IL-12、TNF-α水平；同时将树突状细胞与淋巴结T细胞共培养，在共培养的不同时间点ELISA法检测培养上清中IFN-γ含量。结果正常死亡的巨噬细胞和S2株感染死亡的巨噬细胞与树突状细胞共培养后均可刺激树突状细胞分泌TNF-α，但S2株感染死亡组的TNF-α分泌水平明显高于正常死亡组（P<0.05），但不刺激IL-12分泌，且与T细胞共培养各时间点IFN-γ的分泌水平均高于对照组（P<0.05）。结论感染布鲁菌S2株死亡的巨噬细胞可激活树突状细胞，并可使其提呈抗原、诱导T细胞产生IFN-γ。

关键词: 布鲁菌, 巨噬细胞, 树突状细胞, T细胞

Abstract: Objective To study the effects of dead macrophage infected by B. suis S2 to prime T cell immune response. Methods Macrophage was infected for 1 h by B. suis S2 in vitro and the normal macrophge acted as control, and was cultured with serum-free medium for 5 d untill the cell completly dead. The dead macrphage was co-cultured with murine bone marrow-derived dendritic cells (BMDC) for 24 h. The IL-12, TNF-αsecreted by BMDC were detected with indirected ELISA. Meanwhile the BMDC was co-cultured with T cells, and the IFN-γcontent in the culture supernatant was detected by ELISA. Results Both normal dead macrophages and B. suis S2 infected dead macrophages could induce BMDC TNF-αsecretion and the TNF-αsecretion of BMDC in B. suis S2 infected group was signiifcantly increased than that of control group(P<0.05), but IL-12 was not produced. The levels of IFN-γof T cell in B. suis S2 infected group were signiifcantly higher than those of control group at 24 h, 48 h and 72 h (P<0.05). Conclusion The dead macrophage infected by B. suis S2 can activvate DC to secret TNF-αand present antigen to T cell and result in T cell IFN-γsecretion.

Key words: Brucella suis, macrophage, dentritic cell, T cell

中图分类号:

R392

冯娜,王晓曼,王梦,任亚莉,马宁,曹志然. 布鲁菌S2感染后死亡的巨噬细胞启动T细胞应答的作用[J]. 医学研究与教育, 2013, 30(6): 1-4,34.

FENG Na,WANG XiaoMan,WANG Meng,REN Yali,MA Ning,CAO Zhiran. Studies on the effects of dead macrophage infected by Brucella suis S2 to prime T cell response[J]. Medical Reserch and Education, 2013, 30(6): 1-4,34.

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布鲁菌S2感染后死亡的巨噬细胞启动T细胞应答的作用

Studies on the effects of dead macrophage infected by Brucella suis S2 to prime T cell response

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