营养因子对药用植物愈伤组织中次生代谢产物积累的影响

doi:10.3969/j.issn.1674-490X.2015.04.010

医学研究与教育 ›› 2015, Vol. 32 ›› Issue (4): 38-43.DOI: 10.3969/j.issn.1674-490X.2015.04.010

营养因子对药用植物愈伤组织中次生代谢产物积累的影响

张乐¹，朱虹^1,2，房慧勇³，张春红^1,2，李旻辉^1,2

1. 包头医学院，内蒙古包头 014060；2. 内蒙古自治区特色药用植物培育与保护工程技术研究中心，内蒙古包头 014060；3. 河北大学中医学院，河北保定 071000

出版日期:2015-08-25 发布日期:2015-08-25
通讯作者: 李旻辉（1978—），男，北京人，教授，博士，博士生导师，主要从事药用植物资源利用与保护。 E-mail: li_minhui@aliyun. com
作者简介:张乐（1990—）, 男，内蒙古赤峰人，在读硕士，主要从事蒙药分子鉴定与资源保护。 E-mail: 404074256@qq. com
基金资助:
“ 十二五”国家科技支撑计划项目（2012BAI28B02）；包头市科技计划项目（2013X2001）；中医药行业科研专项（201407003）

Influence on medicinal plants secondary metabolites accumulation of nutritional factors

ZHANG Le¹, ZHU Hong^1,2, FANG Huiyong³, ZHANG Chunhong¹, LI Minhui^1,2

1. Baotou Medical College, Baotou 014060, China; 2. Inner Mongolia Research Center of Characteristic Medicinal Plants Cultivation and Protection Engineering Technology, Baotou 014060, China; 3. College of Traditional Chinese Medicine of Hebei University, Baoding 071000, China

Online:2015-08-25 Published:2015-08-25

摘要/Abstract

摘要： 植物次生代谢产物是由植物通过次生代谢产生的一类细胞生命活动或生长发育正常运行的非必需化合物。自然界许多药用植物的有效成分都是植物次生代谢的产物。随着药用植物市场需求的增大，野生植物资源逐年减少，利用愈伤组织获得次生代谢产物是目前解决供不应求最为行之有效的方法。通过查阅近几年的国内外文献，综述了营养因子对药用植物愈伤组织中次生代谢产物积累的影响，以期为进一步研究提供参考。

关键词: 营养因子, 药用植物 , 愈伤组织, 次生代谢产物

Abstract: Plant secondary metabolites are non-essential compounds with the function of cell life activities and normal operation of growth and development, which are produced by secondary metabolism. The effective components of medicinal plant are usually the plant secondary metabolites. With the increase of medicinal plant market demand, wild plant reduced year by year, getting secondary metabolites from callus is the most effective way to solve the short supply situation. In this paper, through consulting the literatures at home and abroad in recent years, the nutritional factors on accumulation of secondary metabolites in medicinal plant callus are reviewed to provide a basis for further study and development.

Key words: neurotrophic factor, medicinal plant, callus, secondary metabolites

张乐，朱虹，房慧勇，张春红，李旻辉. 营养因子对药用植物愈伤组织中次生代谢产物积累的影响[J]. 医学研究与教育, 2015, 32(4): 38-43.

ZHANG Le, ZHU Hong, FANG Huiyong, ZHANG Chunhong, LI Minhui. Influence on medicinal plants secondary metabolites accumulation of nutritional factors[J]. Medical Reserch and Education, 2015, 32(4): 38-43.

参考文献

[1] 谢静. 生物技术与药用植物次生代谢产物[J]. 西南军医, 2007, 9(3): 89-90. [2] 房慧勇, 朱虹, 丁海麦, 等. 影响愈伤组织中次生代谢产物含量的因素研究进展[J]. 中国中药杂志, 2014, 39(15): 28462850. [3] 李琰, 王冬梅, 姜在民, 等. 培养基及培养条件对雷公藤愈伤组织生长和次生代谢产物含量的影响[J]. 林业科学, 2010, 46(5): 64-69. [4] RAO S R, RAVISHANKAR G A. Plant cell cultures: Chemical factories of secondary metabolites[J]. Biotechnol Adv, 2002, 20(2): 101-153. [5] 李琰, 王冬梅, 姜在民. 培养基及培养条件对杜仲愈伤组织生长及次生代谢产物含量的影响[J]. 西北植物学报, 2004, 24(10): 1912-1916. [6] ROSLI N, MAZIAH M, CHAN K L, et al. Factors affecting the accumulation of 9-methoxycanthin-6-one in callus cultures of Eurycoma longifolia[J]. J Forestry Res, 2009, 20(1): 54-58. [7] ZENG Fansuo, WANG Weiwei, ZHAN Yaguang, et al. et al. Establishment of the callus and cell suspension culture of Elaeagnus angustifolia for the production of condensed tannins[J]. Afr J Biotechnol, 2009, 8(19): 5005-5010. [8] 聂秀霞, 张寿文, 谢欢. 培养基成分对彭泽贝母愈伤组织增殖及生物碱含量的影响[J]. 时珍国医国药, 2009, 20(10): 24642466. [9] RAFIQ M, DAHOT M U. Callus and azadirachtin related limonoids production through in vitro culture of neem (Azadirachta indica A. Juss)[J]. Afr J Biotechnol, 2010, 9(4): 449-453. [10] ZHAO Jian, ZHU Weihua, HU Qiu, et al. Enhanced indole alkaloid production in suspension compact callus clusters of Catharanthus roseus: impacts of plant growth regulators and sucrose[J]. Plant Growth Regul, 2001, 33(1): 33-41. [11] 李琰, 杨广隶, 冯俊涛, 等. 碳源及植物生长调节剂对雷公藤愈伤组织生长和次生代谢产物含量的影响[J]. 林业科学, 2010, 46(9): 35-39. [12] 李波, 肖静, 李铁, 等. 不同条件对苜蓿愈伤组织异黄酮含量的影响（简报）[J]. 草地学报, 2007, 15(5): 500-502. [13] SEPEHR M F, GHORBANLI M. Effects of nutritional factors on the formation of Anthraquinones in callus cultures of Rheum ribes[J]. Plant Cell Tiss Org, 2002, 68(2): 171-175.14] DODDS J H, ROBERTS L W. Experiments in plant tissue culture [M]. 2nd. New York: Cambridge University Press, 1986. [15] ZHAO Dexiu, XING Jianming, LI Maoyin, et al. Optimization of growth and jaceosidin production in callus and cell suspension cultures of Saussurea medusa[J]. Plant Cell Tiss Org, 2001, 67(3): 227-234. [16] NAKAGAWA K, KONAGAI A, FUKUI H, et al. Release and crystallization of berberine in the liquid medium of Thalictrum minus cell suspension cultures[J]. Plant Cell Rep, 1984, 3(6): 254-257. [17] LEGHA M R, PRASAD K V, SINGH S K, et al. Induction of carotenoid pigments in callus cultures of Calendula officinalis L. in response to nitrogen and sucrose levels[J]. In Vitro Cell Dev Biol Plant, 2012, 48(1): 99-106. [18] YANG Jun, GONG Zhencai, TAN Xing. Induction of callus and extraction of alkaloid from Yi Mu Cao (Leonurus heterophylus Sw. ) culture[J]. Afr J Biotechnol, 2008, 7(8): 1157-1162. [19] MASOUMIAN M, ARBAKARIYA A, SYAHIDA A. et al. Effect of precursors on flavonoid production by Hydrocotyle bonariensis callus tissues[J]. Afr J Biotechnol, 2011, 10(32): 6021-6029

营养因子对药用植物愈伤组织中次生代谢产物积累的影响

Influence on medicinal plants secondary metabolites accumulation of nutritional factors

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