血府逐瘀方对动脉粥样硬化大鼠MMP-2及TIMP-2的影响

doi:10.3969/j.issn.1674-490X.2014.03.001

摘要/Abstract

摘要： 目的：通过研究血府逐瘀方对动脉粥样硬化大鼠动脉斑块组织基质金属蛋白酶2（MMP-2）及基质金属蛋白酶抑制因子2（TIMP-2）表达的影响，探讨血府逐瘀方抗动脉粥样硬化作用的可能机制。方法将30只SD大鼠随机分为对照组、模型组、血府逐瘀方组。对照组给予正常饲料，采用腹腔注射维生素D3及饲喂高脂饲料的方法建立大鼠动脉粥样硬化模型。实验结束后，取大鼠主动脉粥样硬化组织检测MMP-2和的TIMP-2的表达水平。结果HE染色光镜下显示，对照组动脉壁形态结构完整，模型组动脉内膜粗糙，中膜平滑肌增生排列紊乱，血府逐瘀方组形态介于二者之间；免疫组化观察，与对照组相比，模型组和血府逐瘀方组MMP-2的表达显著升高，TIMP-2的表达显著下降；血府逐瘀方组与模型组相比，MMP-2表达显著降低，TIMP-2表达显著升高。结论血府逐瘀方能抑制动脉粥样硬化的形成，其机制可能与其能下调动脉粥样硬化大鼠动脉粥样硬化组织内的MMP-2的表达及升高TIMP-2表达有关。

关键词: 血府逐瘀方, 动脉粥样硬化, MMP-2, TIMP-2

Abstract: Objective To explore mechanism of Xuefu Zhuyu Decoction on the expression of matrix metalloproteianse-2(MMP-2) and tissue metalloproteinase inhibitor -2 (TIMP- 2) in atheroselerotic rats. Methods 30 SD rats were randomly divided into the control group, model group and Xuefu Zhuyu Decoction group. Subcutaneous injection vitD3 with high fat feeding was conducted to reproduce atheroselerotic rats model. After the experiment, immunohistochemistry staining was performed to detect the level of MMP- 2 and TIMP-2 protein expression. Results Under light microscope, arterial structure of the control group was completed, artery intima of the model group was rough, the smooth muscle was disordered arrangement, Xuefu Zhuyu Decoction forms was between the two;Compared with the control group, the expressions of MMP-2 was increased signiifcantly and the expressions of TIMP-2 was decreased signiifcantly in in model group and Xuefu Zhuyu Decoction group. Compared with the model group, the expressions of MMP-2 protein in Xuefu Zhuyu Decoction group were decreased signiifcantly, the expressions of TIMP-2 protein were increased signiifcantly.Conclusion The Xuefu Zhuyu Decoction Could downregulate the expressions of MMP-2 and upregulate he expressions of TIMP-2 and inhibit the formation of atherosclerosis.

Key words: Xuefu Zhuyu Decoction, Atheroselerosis, MMP-2, TIMP-2

中图分类号:

R363

董超,马新乔,高军秒,李芸. 血府逐瘀方对动脉粥样硬化大鼠MMP-2及TIMP-2的影响[J]. 医学研究与教育, 2014, 31(3): 1-5.

DONG Chao,MA Xinqiao,GAO Junmiao,LI Yun. Effects of Xuefu Zhuyu Decoction on MMP-2 and TIMP-2 in atherosclerotic rats[J]. Medical Reserch and Education, 2014, 31(3): 1-5.

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