血府逐瘀汤对动脉粥样硬化大鼠MMP-9及TIMP-1的影响

doi:10.3969/j.issn.1674-490X.2013.05.003

摘要/Abstract

摘要： 目的：研究血府逐瘀汤对动脉粥样硬化（atherosclerosis, AS）大鼠动脉斑块基质金属蛋白酶9（MMP-9）及组织抑制物1（TIMP-1）的影响。方法将30只SD大鼠随机分为对照组、模型组、血府逐瘀汤组，对照组给予正常饲料，模型组给予高脂饲料，血府逐瘀汤组给予高脂饮食同时给予该药干预。12周后，处死大鼠，取主动脉免疫组化法观察主动脉斑块内MMP-9和TIMP-1的表达水平。结果血府逐瘀汤组MMP-9的表达较模型组明显减少（P<0.05），TIMP-1表达明显升高（P<0.05）。结论血府逐瘀汤可以通过下调动脉粥样硬化大鼠动脉斑块内MMP-9的表达，升高TIMP-1表达，起到防治动脉粥样硬化和稳定斑块的作用。

关键词: 血府逐瘀汤, 动脉粥样硬化, MMP-9, TIMP-1

Abstract: Objective To investgate the effects of Xuefu Zhuyu Decoction on the expression of matrix metalloproteianse-9 and tissue metalloproteinase inhibitor-1 in aorta of atheroselerotic rats. Methods 30 SD rats were randomly divided into the control group model group and Xuefu Zhuyu Decoction group(each n=15), the rats of the control group were given normal diet, that of the model group were given a high fat diet, the rats of Xuefu Zhuyu Decoction group were given a high fat diet and given treatment drugs. After 12 weeks, all rats were killed and expression of MMP-9 and TIMP-1 in atheroselerotic plaque were detected by using immunohistochemistry assay. Results The MMP-9 expression decreased signiifcantly(P<0.05), and TIMP-1 expression increased significantly(P<0.05) in the Xuefu Zhuyu Decoction compared with that of the model group. Conclusion The Xuefu Zhuyu Decoction can decrease the expression of MMP-9 and increase the expression of TIMP-1, it can attenuate atherosclerotic development in rats.

Key words: Xuefu Zhuyu Decoction, atherosclerosis, matrix metalloproteianse-9, tissue metalloproteinase inhibitor-1

中图分类号:

R363

黄威,董超. 血府逐瘀汤对动脉粥样硬化大鼠MMP-9及TIMP-1的影响[J]. 医学研究与教育, 2013, 30(5): 8-12.

HUANG Wei,DONG Chao. Effects of Xuefu Zhuyu Decoction on the expression of matrix metalloproteianse-9 and tissue metalloproteinase inhibitor-1 in atherosclerotic rats[J]. Medical Reserch and Education, 2013, 30(5): 8-12.

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