Effects of different solvents on the polypeptide antigen’s emulsification

doi:10.3969/j.issn.1674-490X.2015.03.001

Abstract

Abstract: Objective To study the effects of different solvents (sterile water and PBS buffer) on the polypeptide antigen’s emulsification. Methods Dissolved polypeptide antigen was completely emulsified with complete Freund’s adjuvant by the way of pull-and-push with an artificial blender connecting three-way pipe and two yringes. The effects of emulsified mixture were evaluated by water drip test. Results The emulsification time was longer and the procedure was easy to control by taking the sterile water as solvent. The stable water in oil emulsifier was formed in a short time by taking the PBS buffer as solvent, whereas it was easy to get over emulsified. Conclusion The effects of different solvents on the polypeptide antigen’s emulsification were different significantly.

Key words: complete Freund's adjuvant, polypeptide antigen, antigen solvent, emulsion affection

CLC Number:

R392

LOU Yongfu, ZHANG Xuejiao, LIU Jialin, WANG Rongai, LIU Shaochuan, MENG Ming. Effects of different solvents on the polypeptide antigen’s emulsification[J]. Medical Reserch and Education, 2015, 32(3): 1-5.

References

[1] 刘洋, 曹雪涛. 免疫佐剂研发和临床转化的现状与发展趋势[J]. 中国肿瘤生物治疗杂志, 2014, 21(2): 192-202.
[2] FREUND J. The mode of action of immunologica1 adjuvants[J]. Adv Tuberc Res, 1956, 7 (10): 130-148.
[3] 霍华德 G C, 凯瑟 M R. 抗体制备与使用实验指南[M]. 张权庚, 张玉详, 译. 北京: 科学出版社, 2010: 20-25.
[4]孟明, 周蕾, 高伟敏, 等. 活化血小板对类风湿关节炎滑膜炎症和增生影响的实验研究[J]. 细胞与分子免疫学杂志, 2012,28(8): 828-833.
[5] 王黎醒, 李小峰, 赵向聪, 等. 血清葡萄糖-6-磷酸异构酶与类风湿关节炎的相关性研究[J]. 中国医药指南, 2013, 11(16):631-633.
[6] IWANAMI K, MATSUMOTO I, TANAKA-WATANABE Y, et al. Crucial role of the interleukin-6/interleukin-17 cytokine axis in the induction of arthritis by glucose-6-phosphate isomerase[J]. Arthritis Rheum, 2008, 58(3): 754-763．
[7] MATSUMOTO I, ZHANG H, YASUKOCHI T, et al. Therapeutic effects of antibodies to tumor necrosis factor-alpha, interleukin-6 and cytotoxic T-lymphocyte antigen 4 immunoglobulin in mice with glucose-6-phosphate isomerase induced arthritis[J]. Arthritis Res Ther, 2008, 10(3): R66.
[8] IWANAMI K, MATSUMOTO I, TANAKA Y, et al. Arthritogenic T cell epitope in glucose-6-phosphate isomerase-induced arthritis[J]. Arthritis Res Ther, 2008, 10(6): R130．
[9] FERNANDEZ P, PETRES S, ME/CHERI S, et al. Strain-transcendent immune to recombinant Var2CSA DBL5- domain block P.falciparum adhesion to placenta-derived BeWo cells under flow conditions [J]. PLoS One, 2010, 5(9): e12558.
[10] 黄小荣, 梁昌盛, 骆晓枫, 等. 抗原-弗氏佐剂乳化实验技术的改进与技巧[J]. 实验室研究与探索, 2013, 32(8): 265-285.
[11] 罗波, 毛樱逾, 段素群, 等. 弗氏佐剂与抗原乳化方法的改良及其应用[J]. 现代预防医学, 2012, 39(5): 1200-1206.
[12] 徐凤宇, 何昭阳. 免疫佐剂的作用机制及其应用[J]. 中国兽医科学, 2007, 37(5): 449-455.
[13] 周莉婷, 赵晶晶, 吴彦卓, 等. 分枝杆菌多糖免疫调节剂研究进展[J]. 药物生物技术, 2011, 18(2): 176-179.
[14] 李瑶瑶, 明恒强. 我国免疫佐剂的研究现状[J]. 兽药与饲料添加剂, 2009, 14(5): 11-14.
[15] 李学钊, 洪场, 王治仓, 等. 免疫佐剂研究进展及前景展望[J]. 中国动物传染病学报, 2014, 22(4): 81-86.
[16] DONAYRE-TORRES A J, ESQUIVEL-SOTO E, GUTIE/RREZ-XICOTE/NCATL MDE L, et al. Production and purification of immunologically active core protein p24 from HIV-1 fused to ricin toxin B subunit in E. coli[J]. Virol J, 2009, 6: 17.
[17] 韩霞, 程新皓, 王江, 等. 阴阳离子表面活性剂体系超低油水界面张力的应用[J]. 物理化学学报, 2012, 28(1): 146-153.
[18] 谭文英, 许勇, 王述洋, 等. 乳化剂及助乳化剂提高生物油/柴油乳化性能[J]. 农业工程学报, 2013, 29(24): 235-242.
[19] LOUDON PT, YAQER EJ, LYNCH DT, et al. GM-C SF increases mucosal and systemic immunogenicity of an H1N1 influenza DNA vaccine administered into the epideim is of non-human primates [J] . PLoS One, 2010, 5(6): e11021.
[20] 牛凤云, 陈会娟, 贾维刚, 等. 肌力康饮对EAMG大鼠血清AchR-Ab及TGF-B1水平的影响[J]. 中华中医药学刊, 2010, 28(1): 80-82.
[21] 钱毅玲, 赵谋明, 赵强忠. 卡拉胶/黄原胶和K+浓度对低脂低盐乳化肠凝胶品质影响的研究[J]. 现代食品科技, 2009, 25(7): 734-737.
[22] 徐小刚, 杜鹃, 罗艳, 等. 乙烯基聚硅氧烷低聚物乳液的制备及应用[J]. 印染助剂, 2013, 30 (6): 18-22.
[23] 宋儒坤, 李斌, 许沙沙, 等. 卵黄高磷蛋白的功能与应用研究[J]. 中国家禽, 2011, 33(16): 40-42.
[24] 郑配国, 马雪涵, 张俊华, 等. CDllb 在小鼠脾脏和淋巴结细胞中的差异表达及其功能[J]. 细胞与分子兔疫学杂志, 2014,30(12): 1271-1273.
[25] 马杰, 张士璀. 卵黄蛋白的结构和功能[J]. 鲁东大学学报: 自然科学版, 2012, 28(3): 252-260.
[26] 张付轩, 张战修. 影响乳化的因素[J]. 中国中医药现代远程教育, 2011, 9(3): 185-186.