Abstract: Objective To compare and evaluate the effectiveness of four methods of intubation in isolated frog heart perfusion. Methods Isolated frog heart specime were prepared by Straub method, BAMU-HARTUNG'S method, right aortic intubation and posterior vena cava+left aorta intubation method, to analyze the differences of four methods in the time of cannulation, time of heart ex vivo, successful rate, survival rate, heart survival time and rate of coagulation. Results Compared with the traditional intubation-Straub method, the two kinds of double-cannula method were increased in the successful rate, survival rate and heart survival time, decreased in the rate of coagulation, and there were significant difference(P＜0.05),but increased in the time of cannulation and isolated heart, and there were significant difference(P＜0.05). Compared with Straub method, right aortic intubation was increased in the successful rate, survival rate and heart survival time, decreased in coagulation rate and the time of cannulation, and there were significant difference(P＜0.05).But the time of isolated heart was no significant difference(P＞0.05). Compared with the two kinds of double-cannula method, right aortic intubation was decreased in successful rate, the time of cannulation and isolated heart, increased in coagulation rate,and there were significant difference(P＜0.05), but heart survival time and survival rate had no significant difference(P＞0.05).Conclusion The comprehensive evaluation showed that the right aortic intubation method had a great advantage in the isolated frog heart perfusion experiment.

Key words: bullfrog, frog heart intubation, straub method, right aortic intubation, double-cannula intubation