Abstract: Objective To establish a mouse model of RA with hGPI325-339 and hGPI469-483 mixed peptides and observe the frequency and mature status of cDC in RA mice.Methods The frequency of cDC and the expression of CD80 and CD86 in spleen and lymph nodes of RA model mice were detected by flow cytometry.Results The frequency of cDC in spleen of RA mice was significantly lower than that of normal control group during the four stages of inflammation.The expression of CD80 was significantly higher than normal control group in the initial stage, peak stage and remission stage of inflammation, and was significantly lower than normal control group in the progressive stage of inflammation.The expression of CD86 was significantly higher than normal control group in all four stages of inflammation.The frequency of cDC in lymph nodes was significantly higher than normal control group in all four stages of inflammation.The expression of CD80 was significantly higher than normal control group at the initial stage of inflammation and lower than normal control group at other three stages.The expression of CD86 was significantly lower than normal control group in the initial, progressive and remission stages of inflammation, and higher than normal control group in the peak of inflammation.Conclusion The antigen presenting role of splenic cDC may be more important than that of lymph nodes in RA.The cDC maturity of spleen and lymph node in RA show a fluctuating phenomenon that increases at first and then decreases.

Key words: rheumatoid arthritis(RA), cDC, CD80, CD86