Abstract: Objective To investigate the effect of Periplaneta americana extract CⅡ-3 in regulating p16^INK4a protein in inducing SKOV3 aging. Methods SKOV3 cells were treated with different concentrations of Periplaneta americana extract CⅡ-3,and the effects of CⅡ-3 on the proliferation of SKOV3 cells were detected by CCK-8 assay. The effect of Periplaneta americana extract CⅡ-3 on the colony formation of SKOV3 cells was determined by colony culture experiments. SKOV3 cell cycle changes after CⅡ-3 action were examined with flow cytometry detection. Senescence changes in SKOV3 cells after CⅡ-3 action were determined with SA-β-Gal staining detects. Western Blotting was used to detect the effects of CⅡ-3 on SKOV3 cells phosphorylated p16^INK4a protein expression. Results Periplaneta americana extract CⅡ-3 could effectively inhibit the proliferation of SKOV3 cells, and the optimal concentration was 80 μg/mL and an action time was 48 h. The colony formation ratio of SKOV3 cells were reduced after the effect of Periplaneta americana extract CⅡ-3 on SKOV3 cells, the proportion of cells blocked in G₀/G₁ phase were increased, the proportion of cells in S phase decreased, the percentage of positive cells of SA-β-Gal was increased and the upregulation of the expression of the aging regulatory molecule p16^INK4a protein were raised. Conclusion Periplaneta americana extract CⅡ-3 could induce senescence in SKOV3 cells by modulating protein expression of the senescence regulatory molecule p16^INK4a.

Key words: Periplaneta americana extract CⅡ-3, SKOV3 cells, senescence, p16^INK4a protein