Effects of blue LED to the amount of TNF-αand IL-6 in liver regeneration in rats

Abstract

Abstract: Objective To investigate the effects of blue light-emitting diodes(LED) to the amount of TNF-αand IL-6 in the serum of liver regeneration rats after partial hepatectomy. Methods 24 Wistar rats were randomly divided into three groups:Sham group, Model group and LED group. The model was established by partial keratectomy and serum TNF-αand IL-6 levels at different time points were measured with ELISA. Results The levels of TNF-αand IL-6 in every group were increased at 1d after operation, and the levels of TNF-αand IL-6 in the groups of Model and LED were higher than those of in Sham group, but the levels of TNF-αand IL-6 in LED group were higher than those of in Model group (P<0.05). The levels of TNF-αand IL-6 increased gradually and reached a maximum level at 3d post operation in Model and LED group (P<0.05) and revered to normal in Sham group . Conclusion Blue LED can promote liver regeneration after partial hepatectomy, its effect is possibly related with increasing the amount of TNF-αand IL-6.

Key words: blue LED, liver regeneration, TNF-α, IL-6

CLC Number:

R657.3

References

[1] 杨光英, 吴晓翠, 孔祥英. 新生儿黄疸治疗进展 [J]. 临床儿科杂志 2003.doi:10.3969/j.issn.1000-3606.2003.04.025
[2] 袁晟光. 白细胞介素6与肝再生 [J]. 华夏医学 2010.doi:10.3969/j.issn.1008-2409.2010.03.038
[3] 成峰, 王学浩, 华志元, 黄蓬, CHENG Feng, WANG Xue-hao, HUA Zhi-yuan, HUANG Peng. TNF-α和IL-6在大鼠部分肝移植术后肝再生的作用 [J]. 南京医科大学学报（自然科学版） 2007.doi:10.3969/j.issn.1007-4368.2007.11.017
[4] 李国钦. 白细胞介素-6与肝再生 [J]. 临床肝胆病杂志 2006.doi:10.3969/j.issn.1001-5256.2006.05.028
[5] 牛培, 武变瑛, 解志考, 李利平, 申文增, 王晶, NIU Pei, WU Bianying, XIE Zhikao, LI Liping, SHEN Wenzeng, WANG Jing. 不同LED单色光源照射妊娠母鼠对其子代学习记忆能力的影响 [J]. 医学研究与教育 2012.doi:10.3969/j.issn.1674-490X.2012.03.001
[6] Sanchez A, Factor VM, Schroeder IS, Nagy P, Thorgeirsson SS. Activation of NF-kappaB and STAT3 in rat oval cells during 2-acetylaminofluorene/partial hepatectomy-induced liver regeneration. [J]. Hepatology 2004, 2(2).
[7] AKHURST B, MATTHEWS V, HUSK K. Differential lymphotoxin-beta and interferon gamma signaling during mouse liver regeneration induced by chronic and acute injury [J]. HEPATOLOGY 2005, 41(02).
[8] HIGGINS G M, ANDERSON R M. Experimental pathology of the liver:restoration of the liver of the white rat following partial surgical removal [J]. Archives of Pathology 1931, 12.
[9] AKERMAN P, COTE P, YANG S Q. Antibodies to tumor necrosis factor-alpha inhibit liver regeneration after partial hepatectomy [J]. American Journal of Physiology 1992, 263(4 Pt 1).
[10] Webber, EM, Bruix, J, Pierce, RH, Fausto, N. Tumor necrosis factor primes hepatocytes for DNA replication in the rat. [J]. Hepatology 1998, 5(5).