Table of Content

25 February 2013, Volume 30 Issue 1

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The protective effects of HO-1 induced by pitavastatin on hepatic inflammation and injury caused by ischemia reperfusion in rats

2013, 30(1):  9-14. 

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Objective To explore the underlying mechanisms and effects of pitavastatin on hepatic inflammation and injury induced by ischemia reperfusion(IR) in rats. Methods Male Sprague-Dawley rats were randomly divided into 4 groups:a sham-operation group, an ischemia?reperfusion (IR) group, a pitavastatin therapy group and a pitavastatin-ZnPP group. Eight hours post reperfusion, blood was collected and hepatic tissue was isolated. Then, we detected the levels of ALT and AST in the serum as well as myeloperoxidase (MPO) in the liver. Additionally, realtime-PCR was performed to assay HO-1, iNOS and ICAM-1 expression. Results IR induced dramatic hepatic inflammation and injury in rats. Compared with the IR group, pitavastatin treatment lowered the levels of ALT, AST and MPO, suppressed the expression of two pro-inflammatory genes such as iNOS and ICAM-1, and improved hepatic lesions. Nevertheless, an HO inhibitor, ZnPP, reversed the protective effects of pitavastatin on IR injury. Furthermore, HO-1 expression was induced by IR and further promoted by statin treatment. Conclusion Mediated by promoted HO-1 expression, pitavastatin plays a protective role for hepatic inflammation and IR injury in rats.

Transfection of TMEM16A gene into TE-1 cells stably by liposome and its identification

2013, 30(1):  15-19. 

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Objective To get stably expressing TMEM16A proteins by transfecting TE-1 cells. Methods TMEM16A gene was transfected into TE-1 cells by Lipofectamine?2000, then we picked up cell clones in the presence of G418. TMEM16A proteins were identified by confocal microscope and western blot. Results After 4 weeks, the cell clones of colony-like formed after G418 screening. The stable TMEM16A-expression TE-1 cell lines were observed by confocal microscope showing signal of GFP co-transfected. Western blot results showed that TMEM16A protein expressed in control and stable TMEM16A-expression TE-1 cells, and the expression was significantly increased in TMEM16A stable cell lines. Conclusion Stable TMEM16A-expression TE-1 cell lines are created with liposome method successfully.

Effects of blue LED to the amount of TNF-αand IL-6 in liver regeneration in rats

2013, 30(1):  20-22. 

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Objective To investigate the effects of blue light-emitting diodes(LED) to the amount of TNF-αand IL-6 in the serum of liver regeneration rats after partial hepatectomy. Methods 24 Wistar rats were randomly divided into three groups:Sham group, Model group and LED group. The model was established by partial keratectomy and serum TNF-αand IL-6 levels at different time points were measured with ELISA. Results The levels of TNF-αand IL-6 in every group were increased at 1d after operation, and the levels of TNF-αand IL-6 in the groups of Model and LED were higher than those of in Sham group, but the levels of TNF-αand IL-6 in LED group were higher than those of in Model group (P<0.05). The levels of TNF-αand IL-6 increased gradually and reached a maximum level at 3d post operation in Model and LED group (P<0.05) and revered to normal in Sham group . Conclusion Blue LED can promote liver regeneration after partial hepatectomy, its effect is possibly related with increasing the amount of TNF-αand IL-6.

Studying the expression of CyclinD1, VEGF, SMA-αin the lung of asthmatic rats

2013, 30(1):  23-28. 

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Objective To observe the expression of CyclinD1、VEGF、SMA-α in the lung through making asthmatic modeling rats. Methods Sixteen males SD rats were randomly assigned into control group and asthma group, each group had eight rats. The mice were sensitized ovalbumin, then they were challenged with ovalbumin to establish the asthmatic model. The lung function were detected. The sections were stained with HE to assess the inflammatory cell infiltrates. Masson staining was used to detect the expression of collagen in the mice lungs. VEGF levels in serum were measured by enzyme linked-immunosorbent assay (ELISA). Immunohistochemistry was used to detect the expression of SMA-α in the mice lungs. Western blot were applied to examine the expression of CyclinD1 in the lungs. Results The bronchial provocation test showed that, in the asthmatic group, the average expiratory resistance increased remarkably, the expression of collagen, VEGF, SMA-α and CyclinD1 was obviously increased than that of the control group(P<0.05). There were positive correlation between CyclinD1 with SMA-α in asthmatic modeling rats(r=0.68, P<0.05), as well that were positive correlation in control group(r=0.54, P<0.05). There were positive correlation between VEGF with collagen in asthmatic modeling rats(r=0.56, P<0.05), as well that were positive correlation in control group(r=0.48, P<0.05). Conclusion CyclinD1and VEGF are playing an important role in airway remodeling.

Protective effect of compound glycyrrhizin on hepatic injury induced by Con A

2013, 30(1):  29-32. 

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Objective To study the protective effect of compound glycyrrhizin (CG) on hepatic injury Induced by Con A. Methods 50 mice of BALB/C species were randomly divided into normal group, model group, high, medium, low-dose CG-treated group (400, 200, 100 mg?kg-1?d-1), CG-treated group were injected into peritoneal cavity, the normal and model groups were given normal saline. After 7 days, beside mice in normal group, other groups were injected with concanavalin A (Con A) 20 mg/kg via the tail vein to damage mice’s liver. After 12 hour, the index of liver and spleen were calculated .Using automatic biochemical analyzer, the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum were examined, using ELISA for determination of the serum cytokines:tumor necrosis factor alpha (TNF-α). Results Compared with model group, high, medium, low-dose CG-treated group could significantly decrease the activity of AST and ALT and the index of liver and spleen in mice, and CG also could significantly reduce the inflammatory cytokines TNF-αlevel. Conclusion CG is effective in protecting immunological liver injury in mice induced by Con A.

Study of the serum adenosine deaminase level in patients with hepatitis

2013, 30(1):  33-35. 

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Objective To study the level of serum adenosine deaminase in patients viral hepatitis. Methods The adenosine deaminase and liver function indexes of the patients were determined by OLYMPUS AU400. Results The rates of increasing serum adenosine deaminase in acute hepatitis,chronic hepatitis, cirrhosis, hepatitis gravis were 55%, 43.8%, 87.9%and 70%, respectively. It was higher in cirrhosis than acute hepatitis, chronic hepatitis(P=0.008, 0.000, respectively). The level of serum adenosine deaminase in cirrhosis patients was higher than that in acute hepatitis patients, chronic hepatitis patients(P=0.003). The serum adenosine deaminase was correlated positively with serum asparate aminotransferase, globulin, the width of portal vein and the thickness of apleen(r=0.214, 0.376, 0.424, 0.306, respectively), it was correlated negatively with serum albumin and cholinesterase (r=-0.405,-0.351, respectively). Conclusion In patients with viral hepatitis, the higher serum adenosine deaminase expressed the decrease of liver synthesization function, it could not reflect the harm of liver cells and cholestasis perfectly.

Ultrasound guided injection of poly Gui alcohol in the treatment of renal cysts (Reports of 24 cases)

2013, 30(1):  36-38. 

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Objective To observe ultrasound guided injection of poly Gui alcohol in the clinical efficacy of treatment of renal cysts. Methods 24 cases of renal cysts were underwent ultrasound guided injection of poly Gui alcohol to evaluate the clinical effect. Results 24 cases were first puncture success, out of cyst fluid 20-150ml, yellow transparent to the main cyst, only a few light red, after injection there was no significant systemic reactions and local discomfort after 3 months in 4 patients cyst although the reduced diameter than the first quarter of the puncture, but still larger than 4cm, after repeat puncture and 6-month follow-up, the cysts of 6 case disappeared, the cyst diameter 16 cases were 2-3cm, 1 patients remained greater than 4cm, 1 were lost. All patients had no adverse reactions. Conclusion Ultrasound guided injection of poly Gui alcohol it’s ideal is a good minimally invasive method of treatment, it has slight side effects. In particular, for the patients who can not tolerate surgery.

Expression of filamin A and its relationship with lymphatic microvessel density in colorectal adenocarcinoma

2013, 30(1):  39-42. 

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Objective To observe the expression level of filamin A (FLNa) and lymphatic microvessel in human colorectal adenocarcinoma tissues,and analyze their correlation. Methods The expression of FLNa and D2-40 in colorectal adenocarcinoma tissues and colorectal normal tissues was examined by immunohistochemistry. We also investigated the correlation between their expression and the clinicopathologic features. Results The positive expression of FLNa in cancer tissues was lower than that in normal colorectal tissues, while the LMVD values in colorectal cancer tissues was higher than that in normal colorectal tissues. Expression of FLNa and LMVD value was correlated with liver metastasis, lymph node metastasis, and the depth of tumor invasion.The mean LMVD in group with FLNa positive was significantly lower than in that with FLNa negative. Conclusion During the occurrence and development of colorectal adenocarcinoma, there is a close relationship between the expression of FLNa and lymphatic microvessel of tumor.

Application of tumor markers combined with spiral CT to diagnosis of mediastinum lymph node metastasis in lung cancer

2013, 30(1):  43-47. 

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Objective To analyze the MLNM detection rate, sensitivity and accuracy with spiral CT scanning, tumor markers and both of them and assess the value of the combination of tumor markers and CT scanning in clinic diagnosis of MLNM in lung cancer. Methods 110 cases (male 64, female 46, aged 41 to 47) hospitalized in Baoding No.3 hospital from September 2010 to August 2011 were selected. Radiations were taken with a Siemens spiral CT machine, under 130 kV, 110 mAs. C-12 protein chip system for multi-tumor marker detection was used. Results Positive diagnosis of MLNM with CT was 33, with sensitivity of 67.35%and specificity of 70.49%. As to tumor markers, the sensitivity was 77.55%and the specificity was 59.02%. When combined, the sensitivity was significantly higher (P<0.01) up to 91.84%, and the specificity was 65.57%, the accuracy was 77.27%. Conclusion The combination of Multi-Tumor Marker Protein Chip technique and spiral CT, comparing with either single technique, can significantly increase the diagnosis sensitivity and accuracy of MLNM, furthermore increase the diagnosis accuracy of TNM stages in lung cancer.

Clinical observation and its clinical influencing factors on insulin pump intensive treatment of type 2 diabetes mellitus

2013, 30(1):  48-50. 

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Objective To compare the efficacy and its influencing factors of insulin pump treatment with traditional insulin pump therapy in the treatment of type 2 diabetes mellitus. Methods Fifty Type 2 diabetic subjects (after OGTT and insulin c-peptide measurement diagnose) were randomized to treat with continuous subcutaneous infusion pump (CSII) and multiple daily insulin injections (MDI), FPG, 0.5 h PG and 2 h PG value was compared between groups before and after treatment respectively. Results There were no significant difference on the FPG and postprandial blood glucose between insulin pump group and the traditional therapy group before admission (P>0.05). Whether the FPG value, pre-dinner blood glucose value, or postprandial blood sugar value, there were not significant difference between the two groups after treatment (P<0.01). Conclusion Insulin pump can simulate human physiological insulin secretion, control the blood glucose level effectively, greatly improve the life quality of patients with diabetes.

Clinical application of UF-1000i fully automatic urinary sediment analyzer in conventional cell counts of hydrothorax and ascites.

2013, 30(1):  51-53. 

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Objective To explore the effects of UF-1000i fully automatic urinary sediment analyzer of Sysmex in the conventional ditection especially in the cell counts of hydrothorax and ascites. Methods To count total number of RBC and WBC in the hydrothorax and ascites of patients respectively by UF-1000i type fully automatic urinary sediment analyzer and traditional manual examined and to compare the testing results of two methods. Results The number of RBC and WBC were respectively (1 542.6±664.7)×106/L and (657.4±104.7)×106/L by detecting with instrument method;The numbers of RBC and WBC were respectively (1 397.6±569.3)×106/L and (635.8±93.3)×106/L by detecting with traditional manual;There were no significant difference between both results by different detecting methods (P>0.05);There were no statistical siggificance comparing between instrument and traditional manual methods. Conclusion It is simple,quick,accurate and important reference value to examine quantitatively cells of hydrothorax and ascites by UF-1000i fully automatic urinary sediment analyzer;It is worthy of clinical apllication.

Clinical observation of Runzaozhiyang capsule combined with Fexofenadine in treating senile skin pruritus

2013, 30(1):  54-56. 

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Objective To observe the curative effects of Runzaozhiyang capsule combined with Fexofenadine oral in the treatment of senile skin pruritus. Methods 128 patients were randomly divided into two groups, 68 cases in treatment group treated with Runzaozhiyang Capsule oral, 4 tablets/time, 3 times/day, combined with Fexofenadine oral, 60mg/time, 2 times/day; 60 cases of the control group, only received fexofenadine oral, 60mg/time, 2 times/day. Two groups were treated for 2 weeks, 4 weeks to judge the curative effects. Results The treatment effectiveness of the treatment group and the control group were respectively 92.65%and 78.33%, there was significant difference(χ2=5.404, P<0.05);The pruritus severity, frequency, duration, pruritus area also had significant difference(P<0.05). Conclusion Runzaozhiyang capsule combined with Fexofenadine in the patients of the two groups have good curative effects and low incidence of adverse reactions in the treatment of senile pruritus.

Clinical efficacy of Huazhuojieduruanganfang combined with ursodeoxycholic acid in treating 60 cases with primary biliary cirrhosis

2013, 30(1):  57-61. 

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Objective To observe the effects on cholestasis indicators of Huazhuo Jiedu Ruangan decoction in patients with primary biliary cirrhosis. Methods 60 cases of patients with primary biliary cirrhosis were randomly divided into two groups, the patients of the control group oraled ursodeoxycholic acid (UDCA);the patients of the treatment group taken Huazhuo Jiedu Ruangan decoction beside UDCA. The effects on cholestasis indicators of Huazhuo Jiedu Ruangan decoction in patients with primary biliary cirrhosis, and the biochemical indicators of cholestasis before and after the treatment were observed. Results The results showed that biochemical indicators in the treatment group improved significantly better than that of the control group, the difference was significant (P<0.05). Conclusion Cholestasis indicators of the patients with primary biliary cirrhosis treated with Huazhuo detoxification decrease significantly.

Qualitative study on the beliefs about physical activity in the community elderly

2013, 30(1):  62-66. 

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Objective To explore beliefs on physical activity in the community elderly and its influencing factors, the focus on their behavioral beliefs normative beliefs, and perceived control beliefs. Methods Half-constructive interview was committed to 12 community elderly. The data was analyzed by Colaizzi method of Phenomena observation qualitative study. Results Three themes was extracted:the physical activity status;physical activity beliefs;physical activity influencing factors. Conclusion Physical activity in the elderly is low, but they have positive attitude toward physical activity, the elders should be given more social and environmental support to raise the level of their physical activity.

Survey on AIDS knowledge, attitudes and behavior in college students in Baoding city

2013, 30(1):  67-70. 

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Objective To understand the status of AIDS knowledge, attitudes and behavior in college students in Baoding. Methods Self-designed questionnaire, random cluster sample of pumping 2 160 institutions in four college students in Baoding were conducted anonymous self-administered questionnaire. Results Respondents on AIDS knowledge and transmission awareness were high, awareness of non-transmission route were low. Respondents/patients with the correct attitude on HIV infection, self-protection attitude and correct behavior of AIDS were lower. 36%of the students received AIDS education in school, major source came from newspapers, magazines, radio and television, followed by classmates and friends, school teachers. 25.25%of the respondents had participated in AIDS prevention public activities. Conclusion College students have good knowledge on AIDS, but the prevalence of the lack of the recognition of the seriousness of the threat of AIDS in sexual behavior, the rete of the students participating in AIDS prevention public activity is not high, college students should have a comprehensive system of health education of AIDS prevention.

Correlation analysis of waist circumference and waist-hip ratio and central obesity of obstructive sleep apnea hypopnea syndrom in male patients

2013, 30(1):  71-73. 

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Objective To analyze the central obesity male patients waistline and waist-hip ratio and with a serious degree of correlation through the measurement of central obesity in patients with obstructive sleep apnea hypopnea syndrom (obstructive sleep apnea - hypopnea syndrome, OSAHS) male patients waist, hip circumference, calculate waist-hip ratio, and the determination of the patients with AHI and minimum blood oxygen saturation level. Methods The waist circumference and hip circumference were measured, waist-hip ratio was calculated, and the patients sleep monitoring process AHI and minimum blood oxygen saturation level in patients with OSAHS waistline and waist-hip ratio and sleep apnea monitoring the linear correlation analysis indicators were conducted. Results Central obesity of OSAHS in the male patients waist circumference and waist-hip ratio and the lowest blood oxygen saturation was negatively correlated with AHI and were positively correlated with AHI (P<0.05). Conclusion Central obesity of OSAHS in the male patients waist circumference and waist-hip ratio can reflect the severity of the illness of the reaction.

Influence of comprehensive nursing intervention on the patients with diastolic heart failure merge anxiety and depression

2013, 30(1):  74-77. 

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Objective To investigate clinical effects of comprehensive intervention on the the patients with diastolic heart failure(DHF) merge anxiety and depression. Methods 100 patients with DHF merge anxiety and depression were selected from Jan. 2010 to Jun. 2011. 100 patients were randomly divided into the control groups(50 cases) and the experimental groups(50 cases). In control groups, the patients were taken conventional therapy and nursing;In experimental groups, the patients were taken conventional therapy and nursing, in addition, taken Individual psychological nursing diet and life style change, instruction of reasonable medication etc.. Then the score of SAS and SDS, the 6-min walk test (6MW), brain natriuretic peptide (BNP), diastolic mitral flow E/A ratio and hospitalization situation in two groups were compared. Results Before intervention indexes had no significant statistic difference in two groups (P>0.05). 3 months later, indexes in the experimental groups were better than indexes in the control groups (P<0.05). Conclusion Comprehensive nursing intervention may reduce the anxiety and depression and improve cardiac function, promote disease rehabilitation, lower medical expense, shorter hospital stays in the patients with diastolic heart failure, and was well worth clinical popularizing and application.

Integrated nursing of Chinese and western medicines for Alzheimer's disease

2013, 30(1):  78-80. 

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With the acceleration of the aging population in our country, patients with Alzheimer’s disease have increased. This problem seriously affected the old people’s physical health. According to the reason and syndrome of the disease, this paper summarized the clinical type of Alzheimer’s Disease. This paper also used corresponding integrated nursing of Chinese and western medicines to control condition, delay the process of Alzheimer’s disease and improve the patient’s daily living ability and the quality of life.

Complications in percutaneous nephrolithotomy

2013, 30(1):  81-86. 

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Based on institutional and personal experience in more than 1 000 patients treated by PCNL, we reviewed the literature (Pubmed search) focusing on technique, type, and incidence of complications of the procedure. Complications during or after PCNL may be present with an overall complication rate of up to 83%, including extravasation (7.2%), transfusion (11.2%–17.5%), and fever (21.0%–32.1%), whereas major complications, such as septicaemia (0.3%–4.7%) and colonic (0.2%–0.8%) or pleural injury (0–3.1%) were rare. Comorbidity (i.e., renal insufficiency, diabetes, gross obesity, pulmonary disease) increased the risk of complications.Most complications (i.e., bleeding, extravasation, fever) could be managed conservatively or minimally invasively (i.e., pleural drain, superselective renal embolisation) if recognised early. The most important consideration for achieving consistently successful outcomes in PCNL with minimal major complications were the correct selection of patients, a well-standardised technique and postoperative follow-up.

Analysis of the causes and preventing methods of knee excessive extension resulted from Stroke-induced Hemiplegia

2013, 30(1):  87-90. 

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The following causes of knee excessive extension resulted from stroke-induced hemiplegia are analysised in this paper:the poor operation ability of the affected side knee, the increasing tension of lower-limb’s extensor, the proprioceptive (deep sensory) disorder, the unscientific training methods and other factors. Rehabilitation strategies for preventing knee excessive extension are also introduced such as the position of normal limb, delaying the time of restanding,the training of improving the stability of knee, decreasing the lower-limb’s tone and the proprioceptive reeducation. It’s indicated that the scientific and standard training are given at the early stage, we can decrease the incidence rates of knee excessive extension abnormal gait to improve the walking ability.

Teaching design of pathophysiology network courses and its application

2013, 30(1):  91-94. 

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To search for a new teaching model of basic medicine which is based on classroom teaching and aided by network teaching, a platform of network course of pathophysiology was designed and set up. Practice and questionnaire survey indicated that application of network course saved some time of, improved the efficiency and enriched the contents of classroom teaching. Application of network course promoted the students self-study ability and the interaction between teaching and learning. The teaching model is feasible and shows a decided advantage over traditional classroom teaching.

Application of PBL teaching method in designing experiment of medical function experiment course

2013, 30(1):  95-98. 

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PBL is problem-based learning mode which promotes active learning. To train the creative thinking of students, it is key point to foster their problem consciousness, and encourage them to query the known and explore the unknown. For the designing part of medical function experiment course, it is preferable to apply the PBL approach. PBL mode exerts significant effect on developing the capacity of the students’ innovation, logical thinking and science research.

Lesson-analyzing practice of function experiment

2013, 30(1):  99-101. 

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Lesson-analyzing is a kind of new teaching reform means. Functional experiment is a new arrangement of courses after teaching reform. The present paper deals with the course setting, teaching goal, teaching process, curriculum evaluation of the functional experiment to explore how to perfect experiment teaching, and make students master basic skills quickly and effectively and improve the comprehensive analysis ability.

Discussion on the reform mode of teaching in experimental curriculum of preventive medicine

2013, 30(1):  102-104. 

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In order to adapt the reform of teaching in preventive medicine to build a new system of practice teaching of preventive medicine and public health, and explore the experimental teaching reform mode of preventive medicine. In teaching content, comprehensive, designing and enlightening experiments should be set up, according to the society needs and students interesting in learning. In teaching methods, modern technological means and multiple teaching methods should be used to mobilize the enthusiasm of students, and pay attention to training students`comprehensive qualities, to achieve the aim of training high quality talents of preventive medicine, for establishing the foundation of theoretical knowledge and practice operation technology of developing innovative research work in the future.

Strengthening the education of traditional culture to improve the quality of medical students

2013, 30(1):  105-108. 

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Traditional culture is the essence of the Chinese nation civilization for thousands of years. The spirit of patriotism, natural spirit of“the Union of Man and Nature”, practical spirit to apply what they learn, struggle spirit of self-improvement, are the essential spirits of the present medical students. In the higher medical education strengthening the education of traditional culture can improve the medical students’ patriotic spirit, cultivation of medical ethics, holistic view, practical spirit and struggle spirit and improve the level of professional skills of medical students and humanistic quality further.