结直肠癌中 PAQR3 甲基化水平及 mRNA 表达的研究

doi:10.3969/j.issn.1674-490X.2015.01.013

医学研究与教育 ›› 2015, Vol. 32 ›› Issue (1): 55-60.DOI: 10.3969/j.issn.1674-490X.2015.01.013

结直肠癌中 PAQR3 甲基化水平及 mRNA 表达的研究

宋艳敏^{1^{，李日恒²，张涛²，贾佳²，马芳²，赵立华²}}

1. 河北大学临床学院，河北保定 071000；2. 河北大学附属医院普通外科，河北保定 071000

出版日期:2015-02-25 发布日期:2015-02-25
通讯作者: 李日恒（1972—），男，黑龙江齐齐哈尔人，副主任医师，博士，硕士生导师，主要从事结直肠及血管外科疾病的基础及临床研究。E-mail: LRH1120@21cn.com
作者简介:宋艳敏（1986—），女，河北沧州人，在读硕士。 E-mail: songyanmin1211@sina.com

The methylation and mRNA expression of PAQR3 in colorectal cancer

SONG Yanmin¹, LI Riheng², ZHANG Tao², JIA Jia², MA Fang², ZHAO Lihua²

1. College of Clinical Medicine, Hebei University, Baoding 071000, China; 2. Department of General Surgery, Affiliated Hospital of Hebei University, Baoding 071000, China

Online:2015-02-25 Published:2015-02-25

摘要/Abstract

摘要： 目的研究结直肠癌组织中抑癌基因PAQR3 mRNA表达情况及甲基化水平，探讨PAQR3基因异常甲基化与结直肠癌的关系。方法收集结直肠癌及正常组织标本 54 组，用逆转录 - 聚合酶链反应（RT-PCR）检测结直肠癌标本中PAQR3 mRNA 表达水平；用甲基化特异性聚合酶链反应（MSP）法检测结直肠癌标本中 PAQR3 基因甲基化水平，用以分析PAQR3 甲基化水平与临床病理资料的关系。结果 54组结直肠癌标本中PAQR3 mRNA表达降低发生率为57.4%（31/54），正常组织 PAQR3 mRNA 表达降低发生率为 18.5%（10/54），表达差异有统计学意义（P= 0.000）；癌组织PAQR3 基因启动子甲基化发生率为 33.3 %（18/54），正常组织 PAQR3 基因启动子甲基化率为 5.6 %（3/54），二者差异有统计学意义（P= 0.000）；PAQR3 基因甲基化与年龄、分化程度、浸润深度、临床分期、淋巴结转移有关，与性别、部位无关。结论结直肠癌组织中 PAQR3 表达降低，与其基因启动子发生高甲基化有关。

关键词: PAQR3, 结直肠癌, 甲基化, MSP

Abstract: Objective To investigate the relationship between the methylation level of PAQR3 and colorectal cancer, the methylation level and mRNA expression of PAQR3 gene in colorectal cancer were detected. Methods 54 colorectal cancers and 54 colorectal normal tissues were collected to detect the expression of mRNA level by reverse transcriptase PCR(RT-PCR), the methylation level of PAQR3 gene were detected by Methylation Specific PCR(MSP), which were used to analyze the relationship between methylation of PAQR3 and clinical data. Results Expression of PAQR3 mRNA in 54 normal tissues were significantly higher than that in 54 colorectal cancer tissues , the expression rates were 18.5%(10/54) and 57.4%(31/54), there was significant difference between cancer and normal tissues(P= 0.000).The PAQR3 gene promoter methylation rate of colorectal cancer and colorectal normal tissues were 33.3%(18/54)and 5.6 %(3/54), there was significant difference between cancer and normal tissues(P= 0.000).There was significant difference in age, differentiation, infiltration, clinical stage and the metastasis of lymph node in colorectal cancer, but not in sex and location. Conclusion PAQR3 expression can be reduced in colorectal cancer, PAQR3 gene promoter hypermethylation may play an important role in oncogenesis of colorectal cancer.

Key words: PAQR3, colorectal cancer, methylation, MSP

中图分类号:

R656.9

宋艳敏，李日恒，张涛，贾佳，马芳，赵立华. 结直肠癌中 PAQR3 甲基化水平及 mRNA 表达的研究[J]. 医学研究与教育, 2015, 32(1): 55-60.

SONG Yanmin, LI Riheng, ZHANG Tao, JIA Jia, MA Fang, ZHAO Lihua. The methylation and mRNA expression of PAQR3 in colorectal cancer[J]. Medical Reserch and Education, 2015, 32(1): 55-60.

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结直肠癌中 PAQR3 甲基化水平及 mRNA 表达的研究

The methylation and mRNA expression of PAQR3 in colorectal cancer

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