LED-蓝光对大鼠肝再生TNF-α、IL-6的影响

医学研究与教育 ›› 2013, Vol. 30 ›› Issue (1): 20-22.

LED-蓝光对大鼠肝再生TNF-α、IL-6的影响

李存占¹,张智¹,段斐¹,申文增¹,牛培¹

河北大学基础医学院,河北保定 071000

收稿日期:2016-10-09 修回日期:2016-10-09 出版日期:2013-02-25 发布日期:2013-02-25

Effects of blue LED to the amount of TNF-αand IL-6 in liver regeneration in rats

Received:2016-10-09 Revised:2016-10-09 Online:2013-02-25 Published:2013-02-25

摘要/Abstract

摘要： 目的探讨LED-蓝光对大鼠肝大部切除肝再生后TNF-α、IL-6含量变化的影响.方法健康雄性Wistar大鼠24只,随机分为3组,假手术组、模型组及LED干预组.采用Higgins改良方法制备大鼠70%肝切除后肝再生的模型,在手术后的不同时间点,检测血清中TNF-α、IL-6的含量变化.结果各实验组动物在手术后1 d血清中TNF-α、IL-6的水平均有升高,模型组和LED干预组的TNF-α、IL-6水平均高于假手术组(P<0.05),且LED干预组的TNF-α、IL-6水平均显著高于模型组;在术后3 d假手术组血清TNF-α、IL-6水平即趋于正常,而模型组和LED干预组TNF-α、IL-6水平达高峰;在术后5 d和7 d,模型组和LED干预组血清中TNF-α、IL-6的水平开始下降,但LED干预组TNF-α、IL-6的水平仍高于模型组.结论LED-蓝光对肝切除后肝再生起明显的促进作用,其机制可能与增强TNF-α、IL-6的含量有关.

关键词: LED-蓝光, 肝再生, TNF-α, IL-6

Abstract: Objective To investigate the effects of blue light-emitting diodes(LED) to the amount of TNF-αand IL-6 in the serum of liver regeneration rats after partial hepatectomy. Methods 24 Wistar rats were randomly divided into three groups:Sham group, Model group and LED group. The model was established by partial keratectomy and serum TNF-αand IL-6 levels at different time points were measured with ELISA. Results The levels of TNF-αand IL-6 in every group were increased at 1d after operation, and the levels of TNF-αand IL-6 in the groups of Model and LED were higher than those of in Sham group, but the levels of TNF-αand IL-6 in LED group were higher than those of in Model group (P<0.05). The levels of TNF-αand IL-6 increased gradually and reached a maximum level at 3d post operation in Model and LED group (P<0.05) and revered to normal in Sham group . Conclusion Blue LED can promote liver regeneration after partial hepatectomy, its effect is possibly related with increasing the amount of TNF-αand IL-6.

Key words: blue LED, liver regeneration, TNF-α, IL-6

中图分类号:

R657.3

李存占,张智,段斐,申文增,牛培. LED-蓝光对大鼠肝再生TNF-α、IL-6的影响[J]. 医学研究与教育, 2013, 30(1): 20-22.

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