不同强度磁场对大鼠外周神经瘢痕形成的影响

医学研究与教育 ›› 2014, Vol. 31 ›› Issue (6): 5-9.

不同强度磁场对大鼠外周神经瘢痕形成的影响

周成¹，姜源涛²，薛金伟²，张珂²，张志军³，陈红刚⁴，杜琳喆⁵，刘双立⁶

1. 河北大学，河北保定 071000； 2. 河北大学附属医院，河北保定 071000；3. 定兴县医院，河北定兴 072650；4. 望都县医院，河北望都 072450；5. 涞源县医院，河北涞源 074100；6. 中国人民解放军第 252 医院，河北保定 071000

出版日期:2014-12-25 发布日期:2014-12-25
通讯作者: 薛金伟（1978—），男，河北保定人，副主任医师，博士，研究生导师，主要从事周围神经损伤临床与基础研究。E-mail: xuexueisen@sohu.com
作者简介:周成（1988—），男，河北保定人，在读硕士，主要从事周围神经损伤临床与基础研究。E-mail: 635671978@qq.com

Effects of different iIntensity of magnetic field on peripheral nerve scar formation in rats

ZHOU Cheng¹, JIANG Yuantao², XUE Jinwei², ZHANG Ke², ZHANG Zhijun³, CHEN Honggang⁴, DU Linzhe⁵, LIU Shuangli⁶

1. Hebei University, Baoding 071000, China; 2. Affiliated Hospital of Hebei University, Baoding 071000, China; 3. Dingxing County Hospital, Dingxing 072650, China; 4. Wangdu County Hospital, Wangdu 072450, China; 5. Laiyuan County Hospital, Laiyuan 074100, China; 6. The 252 Hospital of PLA, Baoding 071000, China

Online:2014-12-25 Published:2014-12-25

摘要/Abstract

摘要： 目的实验旨在观察磁场干预对大鼠外周神经损伤后神经瘢痕形成过程的影响。方法选用清洁 SD 大鼠 80 只，均对其行左侧坐骨神经卡压术，造成神经损伤，术后随机分 4 组，对照组 1 组，实验组 3 组，实验组分别给予 0.2 mT、0.6 mT、1.5 mT强度磁疗，60 min/d，21 d 后测量坐骨神经功能指数（SFI），之后取材，进行Ⅰ型胶原纤维免疫组织化学染色、透射电镜观察，并对实验资料进行统计学处理。结果实验组较对照组神经功能指数更接近正常；神经损伤处Ⅰ型胶原纤维增生少，髓鞘板层厚且走行规则。结论磁场干预可以明显抑制神经瘢痕的形成，促进神经再生及其功能恢复。

关键词: 磁场, 神经损伤, 神经瘢痕, 胶原纤维

Abstract: Objective To observe how magnetic field interference effect on nerve scar formation after nerve injury in rats. Methods 80 SD rats were implemented left sciatic nerve entrapment surgery, with nerve damaged. After surgery, the rats were randomly divided into four groups: 1 control group and 3 experimental groups. Experimental groups were respectively given 0.2 mT’s, 0.6 mT’s, and 1.5 mT’s intensity magnetic therapy, 60 min/d. Sciatic nerve function index measurement (SFI) was tested in 21 days. After drawn, the samples were applied, type collagen fibers immunohistochemical staining, transmission electron microscope and experimental data statistics processing were performed. Results Compared with the control group, the experimental group was closer to normal in nerve function index, with less, type collagen fibers hyperplasia in Nerve lesions, thicker myelin sheath and line rules walking.Conclusion Magnetic field interference can significantly inhibit scar formation, promote nerve regeneration and functional recovery.

Key words: magnetic field, nerve injury, nerve scar, collagen fiber

中图分类号:

周成，姜源涛，薛金伟，张珂，张志军，陈红刚，杜琳喆，刘双立. 不同强度磁场对大鼠外周神经瘢痕形成的影响[J]. 医学研究与教育, 2014, 31(6): 5-9.

ZHOU Cheng, JIANG Yuantao, XUE Jinwei, ZHANG Ke, ZHANG Zhijun, CHEN Honggang, DU Linzhe, LIU Shuangli. Effects of different iIntensity of magnetic field on peripheral nerve scar formation in rats[J]. Medical Reserch and Education, 2014, 31(6): 5-9.

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