父本表观基因组在早期胚胎发育过程中的功能作用

医学研究与教育 ›› 2015, Vol. 32 ›› Issue (2): 61-64.

父本表观基因组在早期胚胎发育过程中的功能作用

赵峥辉¹，石梦雅¹，殷会莹¹，赵鹏辉¹，葛少钦¹,²

1. 河北大学基础医学院，河北保定 071000； 2. 河北大学生殖医学研究所，河北保定 071000

出版日期:2015-04-25 发布日期:2015-04-25
通讯作者: 葛少钦（1967—），男，河北保定人，教授，博士后，硕士生导师，主要从事动物分子与生化、生殖生物学研究。E-mail: gesq67@sina.com
作者简介:赵峥辉（1991—），男，河北秦皇岛人。E-mail: zhenghuizhao91@163.com
基金资助:
河北省自然科学基金资助项目（H2013201259）；河北2013 留学回国人员科研活动项目（C2013005002）; 河北大学创新训练项目（201410075029，201410075073）

The role of paternal epigenome during early embryogenesis

ZHAO Zhenghui¹, SHI Mengya¹, YIN Huiying¹, ZHAO Penghui¹, GE Shaoqin¹，²

1. College of Basic Medical Sciences of Hebei University, Baoding 071000, China; 2. The Institute for Reproductive Medicine of Hebei University, Baoding 071000, China

Online:2015-04-25 Published:2015-04-25

摘要/Abstract

摘要： 胚胎发育是一个复杂的动态变化过程，频繁改变的基因表达最终导致细胞分化和各种细胞系的形成。这些改变由特定细胞中表观遗传因子所调控。一直以来，人们认为父本表观基因组在胚胎发育中的作用范围很局限。然而，近期精子发生过程中表观遗传修饰的研究显示父本表观基因组在早期胚胎发育过程中具有重要作用。任一表观遗传标记建立和（或）维持的轻微异常均会影响早期胚胎发育过程。现主要综述 DNA 甲基化和组蛋白修饰的功能作用，阐明了其在胚胎发育和男性不育中的作用机制，为胚胎植入前遗传学诊断提供一些基础材料。

关键词: DNA 甲基化, 组蛋白修饰, 胚胎发育, 男性不育

Abstract: Embryonic development is a complex and dynamic process with frequent changes in gene expression, ultimately leading to cellular differentiation and formation of various cell lines. These changes are regulated by the epigenetic factors in specific cells. The scope of paternal epigenome contributions during embryogenesis has long been considered limited. However, recent studies investigating epigenetic modifications in the spermatogenesis have provided new insights that paternal epigenome may establish a more critical role for the early developing embryo. Perturbations in the establishment and/or maintenance of any epigenetic marks have been demonstrated to affect early embryonic development. This review mainly focus on the functions of DNA methylation and histone modifications, elucidate the mechanisms in the regulation of embryogenesis and male infertility, which provides some basic information for preimplantation genetic diagnosis.

Key words: DNA methylation, histone modification, embryogenesis, male infertility

中图分类号:

赵峥辉，石梦雅，殷会莹，赵鹏辉，葛少钦. 父本表观基因组在早期胚胎发育过程中的功能作用[J]. 医学研究与教育, 2015, 32(2): 61-64.

ZHAO Zhenghui, SHI Mengya, YIN Huiying, ZHAO Penghui, GE Shaoqin. The role of paternal epigenome during early embryogenesis[J]. Medical Reserch and Education, 2015, 32(2): 61-64.

参考文献

[1] OKANO M, BELL D W, HABER D A, et al. DNA methyltransferases Dnmt3a and Dnmt3b are essential forde novomethylation and mammalian development[J]. Cell, 1999, 99(3): 247-257.
[2] BESTOR T H. Activation of mammalian DNA methyltrans by cleavage of a Zn binding regulatory domain[J]. EMBO J, 1992, 11(7):2611-2617.
[3] SURANI M A. Imprinting and the initiation of gene silencing in the germ line[J]. Cell, 1998, 93(3): 309-312.
[4] PANNING B, JAENISCH R. DNA hypomethylation can activate Xist expression and silence X-linked genes[J]. Genes Dev, 1996, 10(16): 1991-2002.
[5] LI E, BESTOR T H, JAENISCH R. Targeted mutation of the DNA methyltransferase gene results in embryonic lethality[J]. Cell, 1992, 69(6): 915-926.
[6] EGGER G, LIANG Gangning, APARICIO A, et al. Epigenetics in human disease and prospects for epigenetic therapy[J]. Nature, 2004, 429(6990): 457-463.
[7] KELLY T L, LI E, TRASLER J M. 5-aza-29-deoxycytidine induces alterations in murine spermatogenesis and pregnancy outcome[J]. J Androl, 2003, 24(6): 822-830.
[8] HAMMOUD S S, NIX D A, ZHANG Haiying, et al. Distinctive chromatin in human sperm packages genes for embryo development[J]. Nature, 2009, 460(7254): 473-478.
[9] SADAKIERSKA-CHUDY A, FILIP M. A comprehensive view of the epigenetic landscape. Part II: Histone post-translational modification, nucleosome level, and chromatin regulation by ncRNAs[J]. Neurotox Res, 2015, 27(2): 172-197.
[10] ZGU Bing, ZHENG Yong, PHAM A D, et al. Monoubiquitination of human histone H2B: the factors involved and their roles in HOX gene regulation[J]. Mol Cell, 2005, 20(4): 601-611.
[11] JENKINS T G, CARRELL D T. The paternal epigenome and embryogenesis: poising mechanisms for development[J]. Asian J Androl, 2011, 13(1): 76-80.
[12] BERNSTEIN B E, MIKKELSEN T S, XIE Xiaohui, et al. A bivalent chromatin structure marks key developmental genes in embryonic stem cells[J]. Cell, 2006, 125(2): 315-326.
[13] OKADA Y, SCOTT G, RAY M K, et al. Histone demethylase JHDM2A is critical for Tnp1 and Prm1 transcription and spermatogenesis[J]. Nature, 2007, 450(7166): 119-123.
[14] FENIC I, HOSSAIN H M, SONGNACK V, et al. In vivoapplication og histone deacetylase inhibitor trichostatin-a impairs murine male meiosis[J]. J Androl, 2008, 29(2): 17-185.
[15] ARPANAHI A, BRINKWORTH M, ILES D, et al. Endonuclease-sensitive regions of human spermatozoal chromatin are highly enriched in promoter and CTCF binding sequences[J]. Genome Res, 2009, 19(8): 1338-1349.
[16] BRYKCZYNSKA U, HISANO M, ERKEK S, et al. Repressive and active histone methylation mark distinct promoters in human and mouse spermatozoa[J]. Nat Struct Mol Biol, 2010, 17(6): 679-687.
[17] ROSE N R, KLOSE R J. Understanding the relationship between DNA methylation and histone lysine methylation[J]. Biochim Biophys Acta, 2014, 1839(12): 1362-1372.