消退素D1对大鼠肝缺血再灌注损伤的保护作用

doi:10.3969/j.issn.1674-490X.2021.06.001

医学研究与教育 ›› 2021, Vol. 38 ›› Issue (6): 1-7.DOI: 10.3969/j.issn.1674-490X.2021.06.001

• 基础医学 • 下一篇

消退素D1对大鼠肝缺血再灌注损伤的保护作用

王阳阳¹,赵娜¹,彭雪莹^1,2,李慧^1,2,王景艳¹

1.河北大学附属医院, 河北保定 071000;
2.河北大学临床医学院, 河北保定 071000

收稿日期:2021-11-16 出版日期:2021-12-25 发布日期:2021-12-25
通讯作者: 王景艳(1972—),女,河北涞水人,副主任护师,主要从事心血管疾病护理及护理管理研究。E-mail: wjy211115@163.com
作者简介:王阳阳(1977—),女,河北保定人,副主任医师,博士,硕士生导师,主要从事肝脏疾病研究。 E-mail: wangyangyang2019@126.com
基金资助:
河北省卫生和计划生育委员会2018年重点医学科学研究课题计划项目(20180704);河北大学医学学科培育项目(2020A12);河北大学附属医院青年基金(2016Q001)

Received:2021-11-16 Online:2021-12-25 Published:2021-12-25

摘要/Abstract

摘要： 目的探讨消退素D1(resolvin D1, RvD1)对大鼠肝缺血再灌注损伤(ischemia reperfusion injury, IRI)的作用。方法实验动物随机分为假手术组(Sham组)、 IR组、RvD1组和ZnPP组。自动生化仪检测氨基转移酶水平;苏木精-伊红染色观察肝组织学变化;髓过氧化物酶(myeloperoxidase,MPO)试剂盒测定MPO水平;实时荧光定量聚合酶链反应(quantitative real-time PCR, qRT-PCR)检测细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)水平。结果与IR组相比,RvD1组大鼠ALT(P<0.05)、AST(P<0.01)明显降低,肝组织IRI减轻,肝内MPO活性降低(P<0.05),ICAM-1 mRNA表达下降(P<0.05)。而ZnPP组则与RvD1组表现相反。结论 RvD1通过降低MPO活性及ICAM-1 mRNA表达发挥对大鼠肝IRI的保护作用,且可以被ZnPP所逆转。

关键词: 消退素D1, 缺血再灌注, 髓过氧化物酶, 细胞间黏附分子-1, 肝脏

Abstract: Objective To investigative the effect of resolvin D1(RvD1)in the rat's liver IRI and the possible mechanism. Methods SD rats were divided into 4 groups randomly: Sham group, IR group, RvD1 group(IR+RvD1)and ZnPP group(IR+ RvD1+ZnPP). The levels of ALT and AST in the serum were detected with biochemical analyzer. MPO in the rat's liver were detected with MPO kit. The changes of liver cell were observed with H-E staining. The mRNA level of ICAM-1 in rat's liver were tested with quantitative real-time PCR(qRT-PCR). Results In RvD1 group, the levels of ALT(P<0.05)and AST(P<0.01)were decreased significantly. The swellings and necrosis in the rat liver cells in RvD1 group were observed to alleviate through H-E staining. Compared with IR group, the level of MPO in RvD1 group was reduced by MPO kit. And the mRNA level of ICAM-1 in RvD1 group was measured to decrease by qRT-PCR. Conclusion RvD1 attenuates the IRI in rat's liver by reducing the level of MPO and ICAM-1 and the protective role is reversed by the inhibitor of HO-1, ZnPP.

Key words: resolvin D1, ischemia reperfusion, myeloperoxidase, intercellular adhesion molecule-1, liver

中图分类号:

R575

王阳阳,赵娜,彭雪莹,李慧,王景艳. 消退素D1对大鼠肝缺血再灌注损伤的保护作用[J]. 医学研究与教育, 2021, 38(6): 1-7.

参考文献

[1] WU M Y, YIANG G T, LIAO W T, et al. Current mechanistic concepts in ischemia and reperfusion injury[J]. Cell Physiol Biochem, 2018, 46(4): 1650-1667. DOI: 10.1159/000489241.
[2] JIMÉNEZ-CASTRO M B, CORNIDE-PETRONIO M E, GRACIA-SANCHO J, et al. Inflammasome-mediated inflammation in liver ischemia-reperfusion injury[J]. Cells, 2019, 8(10): E1131. DOI: 10.3390/cells8101131.
[3] YE L P, HE S Q, MAO X L, et al. Effect of hepatic macrophage polarization and apoptosis on liver ischemia and reperfusion injury during liver transplantation[J]. Front Immunol, 2020, 11: 1193. DOI: 10.3389/fimmu.2020.01193.
[4] KRASHIA P, CORDELLA A, NOBILI A, et al. Blunting neoinflammation with resolvin D1 prevents early pathology in a rat model of Parkinson's disease[J]. Nat Commun, 2019, 10(1): 3945. DOI: 10.1038/s41467-019-11928-w.
[5] LU Y, XU Q Y, YIN G W, et al. Resolvin D1 inhibits the proliferation of lipopolysaccharide-treated HepG2 hepatoblastoma and PLC/PRF/5 hepatocellular carcinoma cells by targeting the MAPK pathway[J]. Exp Ther Med, 2018, 16(4): 3603-3610. DOI: 10.3892/etm.2018.6651.
[6] NAUTA R J, TSIMOYIANNIS E, URIBE M, et al. Oxygen-derived free radicals in hepatic ischemia and reperfusion injury in the rat[J]. Surg Gynecol Obstet, 1990, 171(2): 120-125.
[7] ZHANG S L, FENG Z J, GAO W D, et al. Aucubin attenuates liver ischemia-reperfusion injury by inhibiting the HMGB1/TLR-4/NF-κB signaling pathway, oxidative stress, and apoptosis[J]. Front Pharmacol, 2020, 11: 544124. DOI: 10.3389/fphar.2020.544124.
[8] MACIEJEWSKA D, DROZD A, SKONIECZNA-Z·YDECKA K, et al. Eicosanoids in nonalcoholic fatty liver disease(NAFLD)progression. Do serum eicosanoids profile correspond with liver eicosanoids content during NAFLD development and progression?[J]. Molecules, 2020, 25(9): E2026. DOI: 10.3390/molecules25092026.
[9] MOLAEI E, MOLAEI A, HAYES A W, et al. Resolvin D1, therapeutic target in acute respiratory distress syndrome[J]. Eur J Pharmacol, 2021, 911: 174527. DOI: 10.1016/j.ejphar.2021.174527.
[10] YARIBEYGI H, ATKIN S L, SIMENTAL-MENDI'A L E, et al. Anti-inflammatory effects of resolvins in diabetic nephropathy: mechanistic pathways[J]. J Cell Physiol, 2019. DOI: 10.1002/jcp.28315.
[11] YARMOHAMMADI F, HAYES A W, KARIMI G. Possible protective effect of resolvin D1 on inflammation in atrial fibrillation: involvement of ER stress mediated the NLRP3 inflammasome pathway[J]. Naunyn Schmiedebergs Arch Pharmacol, 2021, 394(8): 1613-1619. DOI: 10.1007/s00210-021-02115-0.
[12] LI J H, DENG X L, BAI T, et al. Resolvin D1 mitigates non-alcoholic steatohepatitis by suppressing the TLR4-MyD88-mediated NF-κB and MAPK pathways and activating the Nrf2 pathway in mice[J]. Int Immunopharmacol, 2020, 88: 106961. DOI: 10.1016/j.intimp.2020.106961.
[13] 王阳阳, 彭雪莹, 孟杰, 等. 消退素D1预处理对肝缺血再灌注损伤大鼠模型的保护作用及机制[J]. 临床肝胆病杂志, 2021, 37(6): 1373-1378.
[14] 王阳阳, 冯志杰, 岳媛媛,等. 血红素氧合酶对大鼠肝脏缺血再灌注损伤细胞凋亡及相关基因的影响[J]. 中华肝脏病杂志, 2007, 15(12): 922-925. DOI: 10.3760/ j.issn:1007-3418.2007.12.011.
[15] 侯英健, 于哲, 步玉辉, 等. 血红素氧合酶-1介导匹伐他汀对大鼠肝脏缺血再灌注损伤的保护作用[J]. 医学研究与教育, 2013, 30(1): 9-14.
[16] SUN Z P, WANG F Q, YANG Y Y, et al. Resolvin D1 attenuates ventilator-induced lung injury by reducing HMGB1 release in a HO-1-dependent pathway[J]. Int Immunopharmacol, 2019, 75: 105825. DOI: 10.1016/j.intimp.2019.105825.
[17] NDREPEPA G. Myeloperoxidase:a bridge linking inflammation and oxidative stress with cardiovascular disease[J]. Clin Chim Acta, 2019, 493: 36-51. DOI: 10.1016/j.cca.2019.02.022.
[18] YU X W, SHANG H, JIANG Y J. ICAM-1 in HIV infection and underlying mechanisms[J]. Cytokine, 2020, 125: 154830. DOI: 10.1016/j.cyto.2019.154830.
[19] WEISS T S, LUPKE M, DAYOUB R, et al. Augmenter of liver regeneration reduces ischemia reperfusion injury by less chemokine expression, Gr-1 infiltration and oxidative stress[J].Cells,2019, 8(11):E1421. DOI: 10.3390/cells8111421.

消退素D1对大鼠肝缺血再灌注损伤的保护作用

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 13

编辑推荐

Metrics

本文评价

[1]	侯凯,李运曼. 与心肌缺血再灌注损伤相关的新型心肌保护分子靶点研究进展[J]. 医学研究与教育, 2020, 37(3): 1-9.
[2]	陈龙剑,凌丽,何龙. 脑梗死患者脑微出血与血管内皮损伤标志物ICAM-1、eNOS、sTM的相关性[J]. 医学研究与教育, 2019, 36(3): 27-32.
[3]	杨琳,周玉娟,李少春,于柏青,刘福林,周程. 丹七对兔心肌缺血再灌注损伤保护作用的研究[J]. 医学研究与教育, 2013, 30(6): 13-16.
[4]	成月英. 肾缺血再灌注损伤机制研究与药物影响[J]. 医学研究与教育, 2013, 30(5): 75-78.
[5]	马士恒,杨彦改,常亚青,谢晓华. 肝病患者血清腺苷脱氨酶检测的临床意义[J]. 医学研究与教育, 2013, 30(1): 33-35.
[6]	侯英健,于哲,步玉辉,韩艳梅. 血红素氧合酶-1介导匹伐他汀对大鼠肝脏缺血再灌注损伤的保护作用[J]. 医学研究与教育, 2013, 30(1): 9-14.
[7]	陈玉敏,陈涛平,冯浩楼. 脑缺血再灌注损伤机制与治疗现状[J]. 医学研究与教育, 2012, 29(6): 47-54.
[8]	李淑翠,马玉丽,王垣芳. 青藤碱对小鼠脑缺血再灌注损伤的保护作用[J]. 医学研究与教育, 2012, 29(5): 14-17.
[9]	陈玉敏,孙春蒲,冯浩楼. 胰岛素对大鼠急性全脑缺血再灌注损伤NSE及行为学变化的影响[J]. 医学研究与教育, 2012, 29(3): 5-9.
[10]	成月英,史小琴,马晓莉. 银杏叶复方制剂对家兔肾脏缺血再灌注损伤时ICAM-1、P-selectin表达的影响[J]. 医学研究与教育, 2012, 29(2): 21-23,30.
[11]	陈亚鑫,曹志然,唐志远. 加味失笑散对家兔心肌缺血再灌注心律失常的防护作用[J]. 医学研究与教育, 2010, 27(4): 21-23.
[12]	吴素焕,武变瑛. 血府逐瘀汤对心肌缺血再灌注损伤心功能的影响[J]. 医学研究与教育, 2010, 27(4): 11-14,18.
[13]	梁璐,曹国辉,王新平,彭广军,徐军,王仲,于学忠,马遂. 延时低温对急性肺损伤早期炎症反应和肺水的影响[J]. 医学研究与教育, 2010, 27(2): 20-25.