doi:10.3969/j.issn.1674-490X.2017.01.001

Abstract

Abstract: Objective To investigate SLC5A8 gene’s transcription level of mRNA and its methylation status in colorectal cancer.Methods Real-time fluorescence quantitative PCR and methylation specific PCR method(MSP)were used to detect the transcription level of SLC5A8 gene’s mRNA and the methylation level of SLC5A8 gene in 40 cases of colorectal cancer tissues and adjacent tissues.Results The rate of transcription level of SLC5A8 gene’s mRNA in colorectal cancer rate was significantly lower than that in tumor adjacent tissues(χ²=16.241,P<0.05),the positive rate of SLC5A8 gene methylation in colorectal cancer tissues was significantly higher than that in adjacent tissues(χ²=32.237,P<0.05),the transcription level of this gene’s mRNA was negatively correlated with its methylation status.The correlation coefficient was 0.875.Conclusion SLC5A8 gene methylation can significantly reduce the expression of the gene,which may be associated with the occurrence of colorectal cancer.

Key words: SLC5A8, colorectal cancer, gene methylation

CLC Number:

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