Transfection of TMEM16A gene into TE-1 cells stably by liposome and its identification

Abstract

Abstract: Objective To get stably expressing TMEM16A proteins by transfecting TE-1 cells. Methods TMEM16A gene was transfected into TE-1 cells by Lipofectamine?2000, then we picked up cell clones in the presence of G418. TMEM16A proteins were identified by confocal microscope and western blot. Results After 4 weeks, the cell clones of colony-like formed after G418 screening. The stable TMEM16A-expression TE-1 cell lines were observed by confocal microscope showing signal of GFP co-transfected. Western blot results showed that TMEM16A protein expressed in control and stable TMEM16A-expression TE-1 cells, and the expression was significantly increased in TMEM16A stable cell lines. Conclusion Stable TMEM16A-expression TE-1 cell lines are created with liposome method successfully.

Key words: TMEM16A, TE-1, Lipofectamine?2000, confocal microscope, Western blot

CLC Number:

R735.1

References

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