The protective effects of HO-1 induced by pitavastatin on hepatic inflammation and injury caused by ischemia reperfusion in rats

Abstract

Abstract: Objective To explore the underlying mechanisms and effects of pitavastatin on hepatic inflammation and injury induced by ischemia reperfusion(IR) in rats. Methods Male Sprague-Dawley rats were randomly divided into 4 groups:a sham-operation group, an ischemia?reperfusion (IR) group, a pitavastatin therapy group and a pitavastatin-ZnPP group. Eight hours post reperfusion, blood was collected and hepatic tissue was isolated. Then, we detected the levels of ALT and AST in the serum as well as myeloperoxidase (MPO) in the liver. Additionally, realtime-PCR was performed to assay HO-1, iNOS and ICAM-1 expression. Results IR induced dramatic hepatic inflammation and injury in rats. Compared with the IR group, pitavastatin treatment lowered the levels of ALT, AST and MPO, suppressed the expression of two pro-inflammatory genes such as iNOS and ICAM-1, and improved hepatic lesions. Nevertheless, an HO inhibitor, ZnPP, reversed the protective effects of pitavastatin on IR injury. Furthermore, HO-1 expression was induced by IR and further promoted by statin treatment. Conclusion Mediated by promoted HO-1 expression, pitavastatin plays a protective role for hepatic inflammation and IR injury in rats.

Key words: ischemia reperfusion, inflammation, HO-1, Pitavastatin

CLC Number:

R575

References

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