Effects of Xuefu Zhuyu Decoction on MMP-2 and TIMP-2 in atherosclerotic rats

doi:10.3969/j.issn.1674-490X.2014.03.001

Abstract

Abstract: Objective To explore mechanism of Xuefu Zhuyu Decoction on the expression of matrix metalloproteianse-2(MMP-2) and tissue metalloproteinase inhibitor -2 (TIMP- 2) in atheroselerotic rats. Methods 30 SD rats were randomly divided into the control group, model group and Xuefu Zhuyu Decoction group. Subcutaneous injection vitD3 with high fat feeding was conducted to reproduce atheroselerotic rats model. After the experiment, immunohistochemistry staining was performed to detect the level of MMP- 2 and TIMP-2 protein expression. Results Under light microscope, arterial structure of the control group was completed, artery intima of the model group was rough, the smooth muscle was disordered arrangement, Xuefu Zhuyu Decoction forms was between the two;Compared with the control group, the expressions of MMP-2 was increased signiifcantly and the expressions of TIMP-2 was decreased signiifcantly in in model group and Xuefu Zhuyu Decoction group. Compared with the model group, the expressions of MMP-2 protein in Xuefu Zhuyu Decoction group were decreased signiifcantly, the expressions of TIMP-2 protein were increased signiifcantly.Conclusion The Xuefu Zhuyu Decoction Could downregulate the expressions of MMP-2 and upregulate he expressions of TIMP-2 and inhibit the formation of atherosclerosis.

Key words: Xuefu Zhuyu Decoction, Atheroselerosis, MMP-2, TIMP-2

CLC Number:

R363

DONG Chao,MA Xinqiao,GAO Junmiao,LI Yun. Effects of Xuefu Zhuyu Decoction on MMP-2 and TIMP-2 in atherosclerotic rats[J]. Medical Reserch and Education, 2014, 31(3): 1-5.

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