Effects of Disobulbin B on proliferation of human gastric carcinoma cell line SGC-7901

Abstract

Abstract: Objective To investigate effects of Disobulbin B (DB) on the proliferation of human gastric carcinoma cell line SGC-7901 and explore the possible mechanism. Methods SGC-7901 cells were treated with different concentration (0～160 μg/mL) of DB. Cell proliferation was detected by CCK-8 assay; cell morphology changes were observed under optical microscopy; The expression of CyclinD1 was detected by Western blot. Results DB inhibited the growth of gastric cancer cells in a does-dependent manner and the highest inhibition rate was 57.26%.The structure of SGC-7901 cells changed greatly. The protein expression level of cyclinD1 was reduced. The effects were increased with the increase of the DB concentration. Conclusion DB could inhibit cell proliferation in SGC-7901 cells by down-regulating the expression of CyclinD1.

Key words: Disobulbin B, gastric carcinoma, SGC-7901 cells, proliferation, CyclinD1 protein

CLC Number:

R735.2

DING Xiaoxu, CHENG Shujie. Effects of Disobulbin B on proliferation of human gastric carcinoma cell line SGC-7901[J]. Medical Reserch and Education, 2015, 32(2): 11-15.

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