Table of Content

25 April 2015, Volume 32 Issue 2

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Effect of AM fungi and selenium content on seeding quality of Salvia miltiorrhiza Bge

QIAO Yajun, GAO Ying, LAN Dandan, LIANG Jie, LI Jianheng

2015, 32(2):  1-5. 

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Objective To explore the effects of AM fungi on the quality of Salvia miltiorrhiza under different selenium levels.Methods Salvia miltiorrhiza seedlings under different conditions were obtained by pot experiment, and the content of polysaccharide, selenium and tanshinone II_A were determinated to evaluate the quality of plants.Results Inoculation of AM fungi significantly increased polysaccharide and selenium content of Salvia miltiorrhiza under the same selenium level. It can be seen AM fungi can promote the accumulation of nutrients, improve the uptake of nutrient elements. The contents of tanshinone II_A in inoculated plants were higher than non inoculated plants. While the selenium concentration was 40 μg/mL, the content of tanshinone II_A in inoculated plants was highest, that was 0.272%. On the different selenium levels, the content of polysaccharide and tanshinone II_A increased firstly and then decreased with increasing selenium concentration, but the contents of inoculated plants were higher than non inoculated plants, they reached the maximum at Se₁(20 μg/mL)-Se₂(40 μg/mL), showed that high selenium inhibited the accumulation of medicinal components. From that proper concentration of selenium was cooperated with AM fungi on improving the accumulating of polysaccharide and medicinal components of Salvia miltiorrhiza. Selenium content in Salvia miltiorrhiza Bge was positively correlated with selenium concentrations, but no obvious linear relationship, the distribution was more in the leaf than root. Conclusion Inoculation of AM fungi on the Salvia miltiorrhiza had the best effects when the selenium concentration was 20-40 μg/mL.

Anti-injury effect of scutellarin on the hypoxic ischemic brain damage of neonatal rats and the expression of p38 MAPK

WANG Lin, NIU Pei, WU Bianying

2015, 32(2):  6-10. 

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Objective To study the effects of scutellarin treat HIBD in neonatal rats and the expression of p38 MAPK signal pathway following HIBD and its mechanism. Methods 45 healthy 7-day-old neonatal SD rats were randomly divided into sham operation group, HIBD group, and scutellarin group(25 mg/kg). After medication, each group respectively executed 5 rats in 24 h, 3 d, 7 d, and took brain tissue slice . The neuronal apoptosis was detected using TUNEL assay. The highest expression and expressional tendencies of p38 MAPK was determined by the method of immunohistochemistry. Results The apoptotic neurons, actived p38 MAPK increased after HIBD in the hippocampal CA1 region. The expression level of p38 MAPK and apopotic neurons in scultellarin group were significantly lower than HIBD group (the differences of the same time point were statistically significant P<0.05 ). Conclusion Scultellarin could reduce the apoptosis through inhibiting the p38 MAPK signal pathway with cerebral hemorrhage.

Effects of Disobulbin B on proliferation of human gastric carcinoma cell line SGC-7901

DING Xiaoxu, CHENG Shujie

2015, 32(2):  11-15. 

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Objective To investigate effects of Disobulbin B (DB) on the proliferation of human gastric carcinoma cell line SGC-7901 and explore the possible mechanism. Methods SGC-7901 cells were treated with different concentration (0～160 μg/mL) of DB. Cell proliferation was detected by CCK-8 assay; cell morphology changes were observed under optical microscopy; The expression of CyclinD1 was detected by Western blot. Results DB inhibited the growth of gastric cancer cells in a does-dependent manner and the highest inhibition rate was 57.26%.The structure of SGC-7901 cells changed greatly. The protein expression level of cyclinD1 was reduced. The effects were increased with the increase of the DB concentration. Conclusion DB could inhibit cell proliferation in SGC-7901 cells by down-regulating the expression of CyclinD1.

Short-term results of ARCH plate in expansive open door laminoplasty (EOLP)

HAN Ye, GAO Wenshan, WU Xiaoming

2015, 32(2):  16-19. 

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Objective To evaluate the short-term results of ARCH plate in EOLP for CSM. Methods From July 2012 to July 2014, 35 patients with multilevel CSM who underwent EOLP via ACTH plate were involved in this study, including 20 males and 15 females. Clinical symptoms were evaluated with Japanese Orthopaedic Association(JOA)scores. Results The follow-up time ranged from 7 to 24 months, with an average of 13.3 months, JOA score were improved from 6.1±2.6 pre-operation to 9.6±2.8 post- operation(P＜0.01). Conclusion For CSM, EOLP using ARCH plate fixation bring less occurrence of re-close of door, slight postoperative neck AS, and satisfactory clinical outcomes.

Comparison of the priming principle of cisatracurium and increasing the dose in duration of anesthesia effect

WU Yi, LI Yongle, WANG Hongjie

2015, 32(2):  20-23. 

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Objective To study the effects of the priming principle of cisatracurium and increasing the dose on the onset time and the clinical duration. Methods 90 patients ASAⅠ-Ⅱ, aged 18-55 years, scheduled for elective gynecologic surgery were randomly allocated to three groups with 30 cases each. GroupⅠ received cisatracurium 0.15 mg/kg (3ED95). Group Ⅱ received cisatracurium 0.015 mg/kg four minutes before induction and then cisatracurium 0.135 mg/kg. Group Ⅲ received cisatracurium 0.2 mg/kg(4ED95). Neuromuscular block was monitored by a TOF Watch SX accelerograph. The onset time and the clinical duration were recorded.Results The onset times for groupⅠ , Ⅱ and Ⅲ were (184±56) s、 (141±31) s、 (135±45) s seconds respectively. Compared with group I , there was significant difference in group Ⅱ, Ⅲrespectively (P<0.05); the clinical effective time were (41±8) min, (44±6) min, (53±8) min respectively. Compared with that of group Ⅲ, there was a remarkable difference in groupⅠand group Ⅱ(P<0.05). Conclusion The priming principle can significantly shorten cisatracurium onset time of intubation. Compared with increasing the dose, its clinical duration is shorter.

Application research on BNP in acute myocardial infarction patients with primary percutaneous coronary intervention

TIAN Zhiyu, ZHAO Wenping, JIA Xinwei, XU Jingjing, PAN Huanjun, LI Ya, ZHAO Shujun

2015, 32(2):  24-29. 

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Objective To investigate the relationship between plasma Brain natriuretic peptide(BNP) level and left ventricular wall motion score index (LVWMSI) in patients with acute ST-segment elevation myocardial infarction (STEMI), and the short term prognosis within 180 days. Methods Sixty four patients with STEMI were treated with primary percutaneous coronary intervention (p-PCI) within 12 h of onset. The BNP level was measured on admission. Based on the BNP level, all patients were divided into four groups (according to the 4 percentile): group A (BNP≤P25: BNP≤115.25 pg/mL), group B(P26≤BNP≤P50: 115.26-200.5 pg/mL), group C(P51≤BNP≤P75: 200.6 -409 pg/mL)and group D(BNP≥P76: BNP≥409 pg/mL). The relationship of BNP level with LVWMSI was analysised. The major adverse cardiac events (MACE) within 180 days were also analyzed. Results The difference of left ventricular wall motion score index among different groups were statistical significant (P<0.05). The levels of BNP and LVWMSI was positively related(r=0.73, P<0.01).Along with the increase of plasma BNP level, major adverse cardiac events increased significantly (P<0.05). Conclusion Plasma BNP level provides information about the change of heart function and short term prognosis for acute myocardial infarction patients.

Evaluation of neurophysiological examination for cerebral function prognosis in severe cerebrovascular disease

WANG Qian, SU Likai

2015, 32(2):  30-34. 

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Objective To investigate the value of electroencephalography, somatosensory evoked potential and brainstem auditory evoked potential on the evaluation of cerebral function of patients with severe cerebrovascular disease. Methods In 24 hours after admitted to hospital, the patients with severe cerebrovascular disease were examined by electroencephalography, somatosensory evoked potential and brainstem auditory evoked potential. The prognosis of cerebral function were evaluated 3 months after the onset of severe cerebrovascular disease or when the patients died in 3 months. Analyze the relationships between the results of the exams above and cerebral function prognosis. Results Ordinal logistic regression analysis found that electroencephalography(P=0.002), somatosensory evoked potential(P＜0.001) and brainstem auditory evoked potential(P=0.002) have a significant effect on the prediction of cerebral function prognosis of severe cerebrovascular disease patients. Conclusion By using electroencephalography, somatosensory evoked potential and brainstem auditory evoked potential exams can make a prediction of prognosis of severe cerebrovascular disease. This is of great importance in evaluating the illness, predicting prognosis, diagnosing and treating severe cerebrovascular disease.

Changes of retrobulbar vascular hemodynamics in patients with diabetes at different periods

WANG Jing, WEI Xu

2015, 32(2):  35-39. 

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Objective To study the changes of retrobulbar vascular hemodynamics in patients with diabetic retinopathy (DR) by using color Doppler imaging technology (CDI). Methods The diagnosed patients withdiabetic mellitus (DM) were divided into non-diabetic retinopathy (NDR) group, non-proliferative diabetic retinopathy (NPDR) group and proliferative diabetic retinopathy (PDR) group. The healthy patients were servedas control (HC) group. The states of carotid artery (CA), ophthalmic artery (OA), central retinal artery (CRA) and central retinal vein (CRV) blood flow parameters were examined with CDI and the results were analyzed. Results Compared with HC group, the blood flow of carotid artery(CA), ophthalmic artery(OA) and the intimamedia thickness (IMT) changes were not obvious in the patients with non-diabetic retinopathy(NDR), but the appearance of CRA peak systolic velocity (PSV) and end diastolic velocity (EDV)increased (P<0.05); phase NDR, CRV increased velocity (P<0.05). In NPDR group and PDR group, PSV, EDV in CA, OA and CRA decreased (P<0.05), resistance index (RI) and pulsatility index (PI) increased (P<0.05). Conclusion There is a reference value for the early diagnosis of DR using color Doppler imaging, but it has limitations in the accurate judgment of retinal blood circulation, however, it can be applied to provide some important information for prognosis and analysis of the degree of lesion.

Risk classification of plasma β₂-microglobulin, high-CRP, HCY and peripheral atherosclerosis disease

JI Jinggang, LIU Haiyong, SUN Liwei, ZHAO Junqing, GU Xuechang, ZUO Ruiju, CAO Liqiu, BU Yuhui

2015, 32(2):  40-43. 

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Objective To investigate the peripheral atherosclerotic disease (PAD) risk classification and plasma β2-microglobulin (β2-microglobulin, β2-MG) high-sensitivity C-reactive protein (H-CRP) of homocysteine (HCY) relationship. Methods From January, 2010 to May, 2013 96 PAD patients were graded and treated in our hospital. According to the ABI value they were divided into: low risk group (0.7≤ABI≤0.9, n=25), medium risk group (0.4≤ABI＜0.7, n=52), and the high risk group (ABI＜0.4, n=19). 40 healthy persons served as the controls the concentration of β2-microglobulin, high CRP, HCY and their correlation were analyzed. Results The concentration of serumβ2-MG, H-CRP, HCY increased with the ABI stage and the progress, the concentrations were negatively correlated with ABI value with a beta 2-microglobulin H-CRP HCY. Conclusion PAD with the severity of the condition, in the plasma β2-MG H-CRP HCY concentration increased gradually,the joint determination of β2-MG H-CRP HCY and ABI combined with a hierarchical diagnosis and prognosis of patients with PAD is helpful to make sound diagnosis

Analysis on induced abortion among adolescents (Report of 167 cases)

ZHANG Xiaoju, LU Zhiru

2015, 32(2):  44-46. 

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Objective To study the current situations of 167 teenagers induced abortion and try to find out effective intervention measures to protect the physical and mental health of teenagers. Methods From January 2009 to December 2013 in the Outpatient Department of Laiyuan Maternal and Child Health-Care Hospital,3 013 cases voluntarily required abortion, off those 167 cases under the age of 19 were analyzed. Results (1) Adolescent abortion number increased year by year during the past five years, compared with 2009,the numbers in 2013 had doubled, in which drug induced abortion accounted for 58.08%. (2) 19 year olds accounted for the highest proportion (41.23%), hospitality workers accounted for(36.53%). Junior High School graduates(35.33%). Recurrent abortions nearly halved(48.50%). (3) 167 adolescents who had abortions suffered from poor self-esteem average (28.84±3.24), including 81 who had multiple abortions(27.91±3.28), among 86 cases of artificial abortion the first time was below the average teen (29.21±3.17), the difference was statistically significant (P<0.01). Conclusion We should promote the sex education for teenagers and take some steps to improve adolescent female self esteem. Better sex education and psychological counselling after artificial abortions will help avoid a repetition to protect the physical and mental health of teenagers, good and honest sex education campaigns are crucial.

Case analysis on the treatment of congenital dacryocystocele by probing of lacrimal passage

WANG Fengxian, HAN Lipo, ZHANG Yu, LIU Zhenpo, ZHANG Jianli, LIU Xiuping, WANG Zheng

2015, 32(2):  47-49. 

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Objective To discuss the treatment and the time of congenital dacryocystocele through the analysis of clinical manifestations and characteristics of congenital dacryocystocele. Methods Retrospective analysis of congenital dacryocystocele 18cases (20 eyes) treated in our hospital from June 2011 to June 2013 were conducted to summarize the clinical manifestations and characteristics of congenital dacryocystocele and analyze the treatment of congenital dacryocystocele and time choice. Results In 18 cases (20 eyes) of congenital dacryocystocele children, 10 cases of male patients (11 eyes), 8 female children (9 eyes), with an average age of 10.6 days. 6 eyes were cured by dacryocyst massage, irrigation of lacrimal passage, application of antibiotic eye drops and other conservative method. 12 eyes were cured by the one probing of lacrimal passages. 2 eyes were cured by the two probing of lacrimal passages. Conclusion Probing of lacrimal passage can be used to treat congenital dacryocystocele. To a large extent, early operation treatment can prevent severe complications.

Analysis of the clinical effect of acetate ringers in correcting metabolic acidosis in patients with hemorrhagic shock

LIU Dong, CAO Zhiran, LIU Bin

2015, 32(2):  50-53. 

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Objective To compare the effects of acetate ringers solution and lactated ringers solution on metabolic acidosis in patients with hemorrhagic shock. Methods 50 patients with hemorrhagic shock were divided equally into 2 groups randomly(n=25): the acetate ringers solution(group A) and lactated ringers group(group L). Changes of arterial blood gas and volume of NaHCO₃ before and 1, 2, 3, 4h after induction respectively were observed. Results There were no statistics difference in the ASA grade, operation time, the volume of transfusion during operation and hemorrhage volume of both groups(P>0.05). pH in group A changed at time point of T3 respectively compared to group L(P<0.05). Serum lactate level(LAC) decreased at T₂ compared to T0 time in group A(P<0.05). LAC level increased at T₂, T₃, T₄ compared to T0 time in group L(P<0.05). LAC level in group L increased at time point of T₂, T₃, T₄ compared to group A(P<0.05). Volume of NaHCO₃ in group A decreased compared to group L (P<0.05). Conclusion Acetate ringers solution changes pH, decreases serum lactate level and decreases volume of NaHCO₃ during fluid resuscitation in hemorrhagic shock.

Blood rheology changes during hysteroscopic resection of submucous uterine fibroids

SHEN Yan

2015, 32(2):  54-57. 

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Objective To investigate the blood rheology changes during hysteroscopic resection of submucous uterine fibroids and provide a theoretical basis for clinical treatment. Methods From January to December, 2014, 45 cases with submucous uterine fibroids were conducted hysteroscopic surgery in Dingxing hospital, before perfusion 10 min (T₁), perfusion 30 min (T₂), after surgery , blood rheology index level (T₃) were detected. Results All cases were successfully conducted the operation, T₃, T₂ point blood rheology index of whole blood low-cut, middle cut high cut, low-cut reduction of whole blood, blood high shear reduced, plasma viscosity, etc.,erythrocyte rigidity index, aggregation index, deformation index and maximum platelet aggregation compared with the index at T₁, the difference was statistically significant (P<0.05). T₂ point hematocrit compared with T₁ point indicator, the difference was statistically significance (P<0.05). T₃ point high shear whole blood, plasma viscosity, etc., hematocrit, erythrocyte rigidity index, maximum platelet aggregation compared with T₂ each index point, the difference was statistically significant (P<0.05). Conclusion Perioperative blood rheology indicators for monitoring is necessary when longer operative time, more fluid absorption to reduce blood viscosity in patients with uterine fibroids after operation and protect red blood cells , reduce cardiovascular events and deep venous thrombosis.

Nursing and observations on the complications of patients with continuous central venous pressure

BAI Guoxin, GAO Ping, ZHANG Peihua

2015, 32(2):  58-60. 

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Objective To analyze the reasons of complication for patients using continuous central venous pressure (CVP) monitoring and explore the best nursing method. Methods Based on the retrospective analysis the CVP monitoring results of one hundred and thirty-two patients using the data from March 2013 to October 2014 in our hospital, we can judge the level of center venous catheter obstruction, infection, detached catheter and perform nursing intervention based on the analysis of the reasons. Results The results showed that five in one hundred and thirty-two patients in CVP monitoring were completely blocked and the incidence rate was 3.79%; at the same time, two patients were found to be infected and the incidence rate was 1.52%; one patient was found to be detached catheter and the incidence rate was 0.76%. Conclusion Intensive nursing interventions will effectively decrease the rate of complication for patients using CVP monitoring.

The role of paternal epigenome during early embryogenesis

ZHAO Zhenghui, SHI Mengya, YIN Huiying, ZHAO Penghui, GE Shaoqin

2015, 32(2):  61-64. 

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Embryonic development is a complex and dynamic process with frequent changes in gene expression, ultimately leading to cellular differentiation and formation of various cell lines. These changes are regulated by the epigenetic factors in specific cells. The scope of paternal epigenome contributions during embryogenesis has long been considered limited. However, recent studies investigating epigenetic modifications in the spermatogenesis have provided new insights that paternal epigenome may establish a more critical role for the early developing embryo. Perturbations in the establishment and/or maintenance of any epigenetic marks have been demonstrated to affect early embryonic development. This review mainly focus on the functions of DNA methylation and histone modifications, elucidate the mechanisms in the regulation of embryogenesis and male infertility, which provides some basic information for preimplantation genetic diagnosis.

Study on detection methods for ascorbic acid in medicine

ZHENG Pei, ZHANG Qingyun, YUAN Liyun, DONG Xiaodong

2015, 32(2):  65-68. 

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Ascorbic acid is also called Vitamin C. It exists in food, human body fluid and tissues, drugs, and it involves in a series of substance metabolism process and oxidation-reduction reaction. So how to detect the ascorbic acid quickly and accurately is an important topic concerned by the researchers all over the world. The usual detection methods include spectrophotometry, fluorescence method, chemiluminescence method, high efficiency liquid chromatography, electrochemical method, titration, et al. In this article, the study of detection methods for ascorbic acid in recent years is reviewed.

Experimental advance on the treatment of dysmenorrheal with traditional Chinese medicine external therapy

MA Xiaoli, PENG Feifei, WANG Miao, JIANG Zhifeng

2015, 32(2):  69-73. 

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Dysmenorrhea is a common gynecologic disease, which seriously affects the women's daily life and work, and the incidence is rising every year. Therefore, a appropriate treatment of dysmenorrhea is really important for improving women’s health, work efficiency and living quality. Treatment with western medicine has obvious effects, but also a considerable side-effects, which can only relieve symptoms, and easily relapse. Traditional Chinese medicine in the treatment of dysmenorrhea has achieved good effects in both primary and late term. And the widely used method is internal treatment, which has clear effects and mechanism. In recent years, acupoint sticking, moxibustion and other external methods are widely used in clinic. However, researches of the treating mechanism and new preparations are rarely seen. This article summarizes and analyzes the characteristics and progress in experimental research on traditional Chinese medicine external treatment of dysmenorrheal by consulting relevant literature in recent 10 years to provide the reference for the development of clinical application and new preparation of traditional Chinese medicine external treatment of dysmenorrhea.

Analysis of the clinical characteristics of peptic ulcer disease in elderly persons

HE Wenjuan

2015, 32(2):  74-76. 

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The incidence of the aged patients with peptic ulcer is on the rise in our country. And many aspects, such as pathogenic sites, clinical symptoms, complications and pathological characteristics, have obvious characteristics for this disease. Therefore, it is significant to recognize the clinical characteristics of the aged patients with peptic ulcer correctly and early diagnose and treat for them. In this paper, the author presents his many years clinical experiences, and gives a deep analysis of the clinical characteristics of the aged patients with peptic ulcer.

Multiple regression analysis to the post stroke depression’s relative factors

CHEN Shuxia, ZHAO Yongchen, GAO Yueping, QIN Wei, ZHANG Xinwen

2015, 32(2):  77-81. 

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Post stroke depression is the common complication of cerebrovascular disease, whose major characteristics are dilatory, slow in reaction, interest drops and feeling down. It influences the patients' living quality, slows down the recovery of neurological and limb function. According to the retrospective analysis of PSD's related risk factors, this article reminds clinician to avoid high risk factors, intending to guide and adjust therapeutic schedule, decrease PSD’s morbidity and improve patients' living quality.

Progress of Strontium on bone mineral metabolism

MA Jiaqing, YU Meng, ZHANG Haisong

2015, 32(2):  82-86. 

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Strontium, one of the necessary trace elements of human, plays a dual role in the regulation of bone metabolism. It can increase bone formation and inhibit bone resorption. The mechanism has been studied extensively. This paper aims to study the relationship between bone mineral metabolism of strontium, and provide a theoretical basis for a broader clinical application of it.

Current research on bowel preparation method before intravenous pyelography

YANG Jingjing, ZHAO Jianxi

2015, 32(2):  87-93. 

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Bowel preparation before intravenous pyelography is of great significance. Different bowel preparation methods applied in recent years are reviewed, including the enema, orally catharsis and orally catharsis combined enema method. Because the orally catharsis method widely used, this paper focuses the use of western medicine, Chinese medicine and multi-drug catharsis principle, usage, and adverse reactions. The analysis shows that different bowel preparations have different safeties, prices in different people. Doctor should be fully aware of the client's own specific circumstances before they select method for intestinal preparation.

Clinical application of Baliao acupoint in the treatment of dysmenorrhea

CHEN Ying, GAO Wei, ZHENG Pei

2015, 32(2):  94-97. 

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The Baliao acupoint originates from Huangdi Neijing, through which good effects has been obtained in the treatment of dysmenorrhea in recent years. The paper explores some related clinical research data that the Baliao acupoint used in treating dysmenorrhea. The results indicate that it can acquire good curative effects about treating dysmenorrhea by massage, acupuncture, moxibustion, acupoint injection, embedded wire and other treatments in the Baliao acupoint. The paper summarizes the experience of Baliao acupoint to treat dysmenorrhea, providing powerful evidence for the clinical treatment of dysmenorrhea by Baliao acupoint.

Discussion on the drug consultation in outpatient department of hospital attached to university

ZHENG Yuhuan, WU Lijie

2015, 32(2):  98-102. 

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Objective To improve the pharmacy services quality, the drug consultation in outpatient department of the hospital attached to university was analyzed. Methods The counseling records for 866 cases from Jan. 2014 to Dec. 2014 in the window of drug consultation in outpatient department of the affiliated hospital of Changchun University of Science and Technology were retrospectively analyzed. ResultsAmong the cases of drug consultation of the affiliated hospital, the outpatient consultation was dominant (633 cases, accounting for 73.09%) and telephone consultation was auxiliary (233 cases, accounting for 26.91%). Among the consulted persons, the college students were dominant (606 cases, accounting for 69.99%), others were faculty (225 cases, accounting for 25.98%), medical staff (18 cases, accounting for 2.08%) and others (17 cases, accounting for 1.96%); Among the questions consulted, the proportion was as follows, drug usage and dosage (306 cases, accounting for 35.33%), drug interactions (112 cases, accounting for 12.93%), the interval time of drug taking (82 cases, accounting for 9.46%) and adverse reactions (51 cases, accounting for 5.89%). Conclusion The developing of drug consultation in the university has important significance, and it can guide the patients to take medicine safely and effectively. For a comprehensive university, because of the huge amount of students and faculty, the questions of drug consultation cover a wide range, which need to further improve the professional level for pharmacists who work in the university.