doi:10.3969/j.issn.1674-490X.2018.02.004

Abstract

Abstract: Objective To observe the expression mode of long chain non-coding RNA-scarna 4(LncRNA-scarna 4)in bladder urothelial carcinoma and the modulation effects of LncRNA-scarna 4 in T24 and HT1376 cancer cell lines.Methods Samples were collected from bladder urothelial carcinoma tissue(n=6), para-carcinoma tissue(n=6)and T24, HT1376 and SV-HUC-1 cell lines. And the expression of LncRNA-scarna 4 was evaluated by qRT-PCR. T24 and HT1376 cells were cultured and treated with three different processing: Lnc-C group(infected with random sequence siRNA), Lnc-S group(infected with LncRNA-scarna 4 sequence siRNA), and C group(cultured with medium). To detect17 the vitality, apoptotic behavior, and proliferation of these cells, typan blue exclusion test, Hoechst33342 staining and western blot for apoptotic protein Bcl2, and soft agar colony formation assay were used respectively. Western blot method was also used to measure the expression of PI3K/Akt pathway. Results LncRNA-scarna 4 was significantly up-regulated both in bladder carcinoma and T24 and HT1376 cells(P<0.05). After infection, the viable rate was significantly lower in Lnc-S group than Lnc-C group(T24: 42.1%±11.2% vs. 87.3%±8.5%, HT1376: 35.3%±8.2% vs. 81.4%±10.4%, P<0.05). The apoptotic number(/103cells)was significantly increased in Lnc-S group(T24: 88±11 vs. 41±9; HT1376: 79±8 vs. 47±8. P<0.05), and the expression of Bcl2 protein was also increased(T24: 181.4%±20.6% vs. 119.6%±11.3%; HT1376: 213.3%±34.6% vs. 121.4%±24.4%, P<0.05). The colony number was lower in Lnc-S group(T24: 2.6±1.1 vs. 12.5±4.6; HT1376:3.6±2.0 vs. 12.1±2.0, P<0.05). Both the PI3K and Akt protein were significantly decreased in Lnc-S group(P<0.05). Conclusion LncRNA-scarna 4 is aberrantly expressed in bladder carcinoma, which participates in the process of apoptotic facilitation, abnormal proliferation through the PI3K/Akt signal pathway.

Key words: LncRNA, urothelial carcinoma, apoptosis, proliferation

CLC Number:

R394

References

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