Abstract: Objective To study the apoptosis of duodenum of mice infected with Trichinella spiralis MethodsInfection model was established, duodenum tissues of the mice were taken in the time of 3, 5, 7days. The histopathological changes were observed, TUNEL method was applied to detect the duodenum apoptotic index, Western-Blot method was used to detect caspase-3 expression level changes in the duodenum tissue of mice of different infection periods. The apoptosis state induced mouse duodenum tissue was conducted comprehensive evaluation. Results HE staining showed that as the infection time went on, the pathological damages of the duodenum were increased TUNEL results showed that the index of apoptotic cells of the mouse duodenum tissue changed with infection time increased significantly(P<0.05), Western-Blot analysis indicated that Caspase-3 expression levels increased with the duodenum tissue prolonged infection time(P<0.05). Conclusion Adult Trichinella spiralis can induce apoptosis in duodenum tissue of the miceand the time of infection was positively correlated.

Key words: Trichinella, duodenum, apoptosis