人参皂苷Rg1联合阿霉素对K562/ADR细胞增殖及耐药的影响

doi:10.3969/j.issn.1674-490X.2023.01.001

医学研究与教育 ›› 2023, Vol. 40 ›› Issue (1): 1-9.DOI: 10.3969/j.issn.1674-490X.2023.01.001

• 基础医学 • 下一篇

人参皂苷Rg1联合阿霉素对K562/ADR细胞增殖及耐药的影响

唐紫云¹,李敏瑞¹,张成桂²,刘衡³,周玥¹

1.大理大学组织学与胚胎学教研室, 云南大理 671000;
2.云南省昆虫生物医药研发重点实验室, 云南大理 671000;
3.药用特种昆虫开发国家地方联合工程研究中心, 云南大理 671000

收稿日期:2022-12-12 出版日期:2023-02-25 发布日期:2023-02-25
通讯作者: 周玥(1979—),女,云南大理人,教授,博士,硕士生导师,主要从事中药药理学研究。E-mail: zhouyue_120@sina.com
作者简介:唐紫云(1996—),女,云南昆明人,在读硕士,主要从事中药药理学研究。 E-mail: 1597345218@qq.com
基金资助:
国家自然科学基金-地区项目(81860038,81660731);国家自然科学基金-面上项目(81873103)

Received:2022-12-12 Online:2023-02-25 Published:2023-02-25

摘要/Abstract

摘要： 目的探究人参皂苷Rg1对人慢性髓细胞白血病耐阿霉素(adriamycin,ADR)细胞K562/ADR增殖的影响及其对K562/ADR细胞的耐药逆转作用。方法 CCK-8法检测不同浓度的人参皂苷Rg1对K562/ADR细胞增殖的影响;CCK-8法检测人参皂苷Rg1与ADR联用对K562/ADR细胞增殖的影响,计算半数抑制浓度(IC₅₀)和逆转倍数;集落形成实验检测人参皂苷Rg1联合ADR对K562/ADR细胞集落形成能力的影响;流式细胞术检测人参皂苷Rg1联合ADR对K562/ADR细胞周期的影响。结果与对照组相比,各浓度和作用时间的人参皂苷Rg1均可抑制K562/ADR细胞增殖(P<0.001),当人参皂苷Rg1浓度为120 μg/mL,作用时间为48 h时,细胞增殖抑制率最高(P<0.05);与单用ADR相比,人参皂苷Rg1联合ADR作用48 h后,K562/ADR细胞IC₅₀值明显下降(P<0.05),人参皂苷Rg1对K562/ADR细胞的耐药逆转倍数为2.34;与对照组和ADR或人参皂苷Rg1单药作用相比,人参皂苷Rg1联合ADR作用能明显抑制K562/ADR细胞集落形成(P<0.05);与ADR或人参皂苷Rg1单药作用相比,人参皂苷Rg1联合ADR作用可以将细胞周期阻滞在G₀/G₁期。结论人参皂苷Rg1联合ADR能明显抑制K562/ADR细胞增殖并逆转K562/ADR细胞对ADR的耐药性,这可能与细胞周期阻滞于G₀/G₁期有关。

关键词: 人参皂苷Rg1, 阿霉素, K562/ADR细胞, 细胞周期, 耐药

Abstract: Objective To investigate the effect of ginsenoside Rg1 on the proliferation of adriamycin(ADR)-resistant K562/ADR cells in human chronic myeloid leukemia(CML)and its reversal of drug resistance in K562/ADR cells. Methods CCK-8 assay was used to detect the effects of different concentrations of ginsenoside Rg1 on the proliferation of K562/ADR cells. CCK-8 assay was used to detect the effects of ginsenoside Rg1 combined with ADR on the proliferation of K562/ADR cells, and the half maximal inhibitory concentration(IC₅₀)and reversal fold were calculated. Colony formation assay was used to detect the effects of ginsenoside Rg1 combined with ADR on the colony formation ability of K562/ADR cells. Flow cytometry was used to detect the effects of ginsenoside Rg1 combined with ADR on the cell cycle of K562/ADR cells. Results Compared with the control group, the proliferation inhibition rate of K562/ADR cells treated with 120 μg/ml ginsenoside Rg1 for 48 h was significantly increased(P<0.001). Compared with the ADR alone group, the IC₅₀ value of ginsenoside Rg1 combined with ADR for 48 h decreased significantly in K562/ADR cells(P<0.05), and the fold reversal of ginsenoside Rg1 resistance to K562/ADR cells was 2.34. Compared with the control group and ADR or ginsenoside Rg1 alone group, ginsenoside Rg1 combined with ADR could significantly inhibit K562/ADR cell colony formation(P<0.05). Compared with the ADR or ginsenoside Rg1 alone group, ginsenoside Rg1 combined with ADR could arrest the cell cycle in G₀/G₁ phase. Conclusion Ginsenoside Rg1 combined with ADR can significantly inhibit the proliferation of K562/ADR cells and reverse the resistance of K562/ADR cells to ADR, which may be related to the cell cycle arrest in G₀/G₁ phase.

Key words: ginsenoside Rg1, adriamycin, K562/ADR cells, cell cycle, drug resistance

中图分类号:

R733.72

唐紫云,李敏瑞,张成桂,刘衡,周玥. 人参皂苷Rg1联合阿霉素对K562/ADR细胞增殖及耐药的影响[J]. 医学研究与教育, 2023, 40(1): 1-9.

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人参皂苷Rg1联合阿霉素对K562/ADR细胞增殖及耐药的影响

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