DTL的亚细胞定位研究

doi:10.3969/j.issn.1674-490X.2023.03.001

医学研究与教育 ›› 2023, Vol. 40 ›› Issue (3): 1-7.DOI: 10.3969/j.issn.1674-490X.2023.03.001

• 基础医学 • 下一篇

DTL的亚细胞定位研究

唐钰梅¹,任来峰²,黄翔³,李强³,程乐¹

1.大理大学基础医学院, 云南大理 671000;
2.山西省肿瘤医院免疫室, 山西太原 030003;
3. 山西省妇女儿童保健院生殖医学中心, 山西太原 030000

收稿日期:2023-03-15 出版日期:2023-06-25 发布日期:2023-06-25
通讯作者: 程乐(1978—),男,山西太原人,副研究员,博士,博士生导师,主要从事肿瘤早期预测和防治与精准医学研究。E-mail: chengle@bgi.com
作者简介:唐钰梅( 1996—),女,湖北恩施人,在读硕士,主要从事肿瘤早期预测和防治与精准医学研究。 E-mail: 1757472744@qq.com
基金资助:
国家重点研发计划课题(2016YFC0900503)

Received:2023-03-15 Online:2023-06-25 Published:2023-06-25

摘要/Abstract

摘要： 目的研究无齿蛋白同源物(denticleless protein homolog, DTL)的亚细胞定位变化,推测其可能的生物学意义。方法在多种细胞和组织中对DTL的亚细胞定位进行免疫荧光(immunofluorescence, IF)和免疫组织化学(immunohistochemistry, IHC)检测,并通过分步提取细胞成分和蛋白质印迹(Western blotting, WB)进行实验验证。结果 DTL在所有细胞的有丝分裂期,其亚细胞定位到细胞核染色质周围;在细胞的分裂间期,DTL在不同细胞和组织中亚细胞定位也有差别,DTL在正常细胞、组织以及精子细胞和小鼠精母细胞中主要表达在细胞核中,在大多数癌细胞、癌组织以及精原细胞中表达在细胞质中。结论 DTL亚细胞定位随着细胞周期发生转变,在不同类型细胞和组织也会发生转变,DTL随着细胞的增殖、分化存在质核转位的趋势,提示DTL可能通过亚细胞定位改变在细胞增殖、分化中发挥作用。

关键词: 无齿蛋白同源物, 亚细胞定位, 细胞周期, 增殖, 分化

Abstract: Objective To explore the subcellular localization of denticleless protein homolog(DTL)and determine the possible biological role of DTL. Methods The subcellular localization of DTL was detected with immunofluorescence(IF)and immunohistochemistry(IHC). Cell fraction extraction and Western blotting(WB)in a variety of cells and tissues were used to verify. Results It was confirmed the subcellular localization of DTL during mitosis was around chromatin in all cells. In addition, our study firstly found the subcellular localization of DTL in different cells and tissues was also different. DTL was mainly expressed in nucleus in normal cells, normal tissues, sperm cells and mouse spermatocytes, and in cytoplasm in most cancer cells, cancer tissues and spermatogonia. Conclusion The subcellular localization of DTL would change not only in the cell cycle, but also in different types of cells and tissues. DTL has the translocation between cytoplasm and nuclear with cell proliferation and differentiation, suggesting that DTL may play a role in cell proliferation and differentiation by changing subcellular localization.

Key words: denticleless protein homolog, subcellular localization, cell cycle, proliferation, differentiation

中图分类号:

R339.2

唐钰梅,任来峰,黄翔,李强,程乐. DTL的亚细胞定位研究[J]. 医学研究与教育, 2023, 40(3): 1-7.

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DTL的亚细胞定位研究

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