Table of Content

25 October 2013, Volume 30 Issue 5

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Effects of Fuzhenghuoxueqingrefang on CAT and MDA in the serum of acute radiation enteritis in rats

HE Jing,ZHAO Zhanxue,ZHANG Rongzhen,DUAN Fei,YANG Yongbin

2013, 30(5):  1-3,7.  DOI: 10.3969/j.issn.1674-490X.2013.05.001

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Objective To investigate the treating mechanism of Fuzhenghuoxueqingrefang on acute radiation enteritis(ARE)in rats. Methods Thirty two Wistar male rats were randomly divided into 4 groups(8 rats each group), which were composed of blank control, model, dexamethasone, and Fuzhenghuoxueqingrefang groups. The physiological saline, dexamethasone and Fuzhenghuoxueqingrefang were administrated separately at the ifrst day after abdominal irradiation by daily rectal clysma for 5 days. All the rats were anesthetized and the serum activity of peroxidase (CAT) and contents of malonic aldehyde(MDA) were detected at the seventh day after abdominal irradiation separately. Results The activity of serum CAT in the blank control, dexamethasone and Fuzhenghuoxueqingrefang groups were obviously higher than that of the model group(F=23.79, P<0.01). The content of serum MDA in the blank control, dexamethasone and Fuzhenghuoxueqingrefang groups were signiifcantly lower than that of the model groups(F=36.41, P<0.01). Conclusion Fuzhenghuoxueqingrefang could treat ARE in rats by down-regulating the lipid peroxidation in the damaged tissue.

Effects of blue LED on the function of the liver regeneration in rats

SHI Qiaodi,NIU Pei,LI Cunzhan,SHEN Wenzeng,DUAN Fei

2013, 30(5):  4-7.  DOI: 10.3969/j.issn.1674-490X.2013.05.002

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Objective To investigate the effects of blue light-emitting diodes(LED) on the liver regeneration in rats with hepatic lobes ligation. Methods 24 SD rats were randomly divided into three groups:Sham group, Model group and LED group. The model was established by ligating partial Liver.The rats of the LED group were shined by LED 30 minutes every day. The serum ALT and AST levels at different time points were measured. After 4 weeks, the weight of liver and rats were measured respectively. Results Compared with Sham group, the results of liver function:ALT, AST markedly increased in model rats(P<0.05) in the ifrst week. There was not any difference in ALT and AST between three groups. The ratio of the total weight of liver to body weight was higher than that of the model group lightened blue light than that in other groups(P<0.05). Conclusion Blue LED can promote liver regeneration and the recovery of the function of liver after ligating partial hepaticlobes.

Effects of Xuefu Zhuyu Decoction on the expression of matrix metalloproteianse-9 and tissue metalloproteinase inhibitor-1 in atherosclerotic rats

HUANG Wei,DONG Chao

2013, 30(5):  8-12.  DOI: 10.3969/j.issn.1674-490X.2013.05.003

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Objective To investgate the effects of Xuefu Zhuyu Decoction on the expression of matrix metalloproteianse-9 and tissue metalloproteinase inhibitor-1 in aorta of atheroselerotic rats. Methods 30 SD rats were randomly divided into the control group model group and Xuefu Zhuyu Decoction group(each n=15), the rats of the control group were given normal diet, that of the model group were given a high fat diet, the rats of Xuefu Zhuyu Decoction group were given a high fat diet and given treatment drugs. After 12 weeks, all rats were killed and expression of MMP-9 and TIMP-1 in atheroselerotic plaque were detected by using immunohistochemistry assay. Results The MMP-9 expression decreased signiifcantly(P<0.05), and TIMP-1 expression increased significantly(P<0.05) in the Xuefu Zhuyu Decoction compared with that of the model group. Conclusion The Xuefu Zhuyu Decoction can decrease the expression of MMP-9 and increase the expression of TIMP-1, it can attenuate atherosclerotic development in rats.

Observation and applied anatomical study of lumbus nerve root

HOU Guozhu,JIA Yitong,SI Daowen,ZHANG Yuxin,CHEN Li,KONG Defu

2013, 30(5):  13-15.  DOI: 10.3969/j.issn.1674-490X.2013.05.004

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Objective To carry anatomical observation and measure on L1 to L5’s length from dural sac’s“axilla”to near-end of ganglion, roughness of nerve root, angle between nerve root axis and midcourt line of dural sac and analyse the clinical signiifcance of nerve roots. Methods In 12 bodies fastened by formalin, we took out vertebral plate in the lumbar vertebra’s superior articular process and inferior articular process with scalpel and lamina impingement rongeur, exposed bilateral dural sac and nerve roots. Isolate nerve roots of L1-5, and conducted elevant observation and measure. Results The nerve root become longer and longer from L1 to L5, L5 was the longest, achieving (12.25±3.66) mm(left), (12.83±2.81) mm(right);roughness become rougher, L5 was the roughest, reaching (3.82±0.88) mm(left), (3.89±0.95) mm(right). The angle of nerve roots became smaller, from (44.33±10.36)°(left), (42.12±10.42)°(right) to (27.33±8.15)°(left), (27.50±8.25)°(right). Conclusion From L1 to L5, the angle between nerve root axis and midcourt line of dural sac become smaller. The length from dural sac’s“axilla”to near-end of ganglion become longer. The roughness of nerve root become rougher. There is no obvious difference between left and right.

Expression of SHP-1 gene and c-kit gene in chronic myeloid leukemia

JI Jing,GUO Ming,HAN Ying,GAO Ming

2013, 30(5):  16-18,27.  DOI: 10.3969/j.issn.1674-490X.2013.05.005

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Objective To explore the expression of SHP-1 gene and c-kit gene in chronic myeloid leukemia and the function in leukemia conversion and prognosis. Methods Semi-quantity reverse transcriptase-polymerase chain reaction(RT-PCR) was used to examine the respective expression of SHP-1 mRNA and c-kit mRNA in the chronic and acute period of chronic myeloid leukemia and normal controls. Results The expression of SHP-1 mRNA in both the chronic period and acute period was less obvious than that of the normal controls, the difference had statistical signiifcance(P<0.05), but the difference between the chronic period and the acute period had no statistical signiifcance(P>0.05) . The expression of c-kit mRNA in the two periods was more obvious than that of the normal controls, the difference had statistical significance(P<0.05), and the expression in the acute period was more obvious than that in the chronic period, the difference had statistical signiifcance(P<0.05). Conclusion SHP-1 gene and c-kit gene may act as the indicators to judge chronic myeloid leukemia conversion and prognosis.

Effects of PTEN gene on the growth and apoptosis of glioma U251 cells

LI Chunhui,ZHENG Kebin,CUI Dongqiang,LI Zhihong,FANG Chuan

2013, 30(5):  19-23.  DOI: 10.3969/j.issn.1674-490X.2013.05.006

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Objective To investigate the effects of PTEN (phosphatase and tensin homologue deleted on chromosome 10) on the growth and apoptosis of glioma cell line U251. Methods The mRNA and protein expressions of PTEN in glioma cell lines transfected by Ad-PTEN were determined by RT-PCR and Western blotting. Apoptosis of cells were detected by MTT. The alterations of p-Akt and P65 protein levels were examined by Western blotting. Results The success of Ad-PTEN transfection were conifrmed by Fluorescence microscopy Western blot and RT-PCR showed apoptosis of glioma cell lines were significantly higher after transfected by Ad-PTEN. The protein expressions of p-Akt and P65 decreased signiifcantly after transfected by Ad-PTEN. Conclusion Overexpression of PTEN will inhabit the growth through promoting cell apoptosis by inhibiting the expression of p-Akt and NF-кB on glioma cell lines.

Effects of Sodium Fusidate on total blile acid

LIANG Shuxin,WANG Shuxian,HAO Wanjiao

2013, 30(5):  24-27.  DOI: 10.3969/j.issn.1674-490X.2013.05.007

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Objective To investigate the effects of Sodium Fusidate on detections of serium total blile acid(TBA). Methods Serium TBA was detected by using the circulating enzymatic method. Distilled water was treated as a control, the amount of TBA was analyzed in different solutions as follows:in serium;in the mixture of Sodium Fusidate and distilled water and in its different concentrations respectively; and in the mixture of Sodium Fusidate and serium and in its different concentrations respectively. Each solution was analyzed triplicatedly. Results The average Serium TBA was 4.4 μmol/L. However, the average amount of TBA in the mixture of Sodium Fusidate and distilled water and in its dilutions of 2, 4, 8, 16 times were 226.53, 130.42, 77.12, 40.78, 21.47 μmol/L respectively. Correspondendly, the average results of Sodium Fusidate serium mixture and its 2, 4, 8, 16 times dilutions were 233.17, 138.82, 85.47, 47.91, 25.58 μmol/L respectively. Conclusion The assays desmontrate that Sodium Fusidate could severely affect the detection of serium TBA and give rise to signiifcant increases. Therefore, it is suggested that those patients who need to know their liver functions should stop taking Sodium Fusidate before doing the tests.

Clinical application of CysC and Hcy detection in diabetic nephropathy

WANG Jianguo,WANG Shuxian,LIANG Shuxin,LI Tiemin

2013, 30(5):  32-34,45.  DOI: 10.3969/j.issn.1674-490X.2013.05.009

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Objective To study the value of clinical application of detection of serum cystatin C(Cysc), hemocysteine(Hcy) in judgement of renal damage of patients with early diabetic nephropathy(DN). Methods According to 24 h urinary albumin excretion rate(UAER), 142 patients with diabetes mellitus(DM) were divided into three groups, including simple diabetes mellitus(SDM), early diabetic nephropathy(EDN) and clinical diabetic nephropathy(CDN) group. Results There were significant differences among the four groups considering the concentration of CysC and Hcy(F=180.273, P<0.05. F=117.064, P<0.05). There were no signiifcant differences between SDM and NC groups connecting the concentration of CysC and Hcy(P>0.05). There were signiifcant differences between EDN, CDN and NC groups connecting the concentration of CysC and Hcy(P<0.05). There was an increased trend in the concentration of CysC and Hcy in the groups of SDM, EDN and CDN. There were signiifcant differences between the groups (P<0.05). Conclusion The combined detection of CysC and Hcy were sensitve indicators in judgement of early renal damage of DN patients, At the same time, they could be used as a monitoring index in patients with DN.

Effects of Naoxintong capsule on CRP, LPa and neural function defect score of ICA

WANG Tao

2013, 30(5):  35-38.  DOI: 10.3969/j.issn.1674-490X.2013.05.010

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Objective To observe the effects of Naoxintong capsule on CRP, LPa and neural function defect score of ICA. Methods 72 patients selected randomly in the Afifliated Hospital of Hebei University were divided into treatment group and control group. The treatment group was treated with Naoxintong capsule on the basis of modern medicine and the control group was treated with modern medicine. The changes of CRP, LPa and neural function defect score were observed. Results The results were both effective in the two groups in the matters of CRP, LPa and neural function defect score. And there was a significant difference between the two groups. The treating effects of the treatment group were better than that of the control group about CRP, LPa and neural function defect score(P<0.01). Conclusion The effects were signiifcant to decrease CRP and LPa and improve neurological function defect by using Naoxintong capsule, which was worthy of applying in clinical practices.

Curative observation on treating secretory otitis media in children by secretory excision

WANG Hongjie

2013, 30(5):  39-41.  DOI: 10.3969/j.issn.1674-490X.2013.05.011

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Objective To discuss the mechanism, time and curative effects in treating secretory ostitis media in children by secretory excision. Methods 78 children admitted into our hospital from July 2010 to July 2012 were selected and diagnosed to be secretory ostitis media because of secretory hypertrophy, among those, 42 received surgery treatment and 36 got non surgery treatment. We compared the treatment effects for those two methods. Results The total efifciency for surgery treatment was 96.62%, and the total efifciency for non surgery treatment was 83.33%, there was great dififdence for the two kind of treatment methods. Conclusion It is beneift for function recovery of eustachian tube, audition improvement after the secretory excision. And the relapse rate is very low, so it is an efifcient method for the treatment of secretory ostitis media in children.

Clinical observation of internal and external treatment with Chinese herbs on hyperplasia of mammary glands

WANG Feiran,ZHANG Caifeng,SHI Shaobing,WEI Xiliang,Li Shuling

2013, 30(5):  42-45.  DOI: 10.3969/j.issn.1674-490X.2013.05.012

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Objective To observe the clinical curative effects of oral administration of traditional Chinese medicine combined with hot compress with herb-medicine residues in treating hyperplasia of mammary glands. Methods 160 cases with hyperplasia of mammary glands were divided into the experimental group and the control group. The patients in the experimental group (80 cases) were treated with Chinese herb internally and externally. The controls (80 cases) were treated with Rupixiao capsules. After three months of treatment, changes of tumor’s size in two groups before and after the treatment as well as the clinical curative effects were observed. Results After the treatment, both of the two groups had decrease in size of tumor (P<0.05). The improvement of the experimental group was higher than that of the controls group (P<0.01). The difference about cure rate and effective rate between the two groups were significant (P<0.05). Conclusion Oral administration of traditional Chinese medicine combined with hot compress with herb-medicine residues has good efifciency in treating hyperplasia of mammary glands.

Observation of narrow red-blue light in the treatment of facial acne

SHEN Juan,QIE Yongtao,WANG Yanjun,ZHOU Jingfang,ZHANG Liping

2013, 30(5):  46-49,61.  DOI: 10.3969/j.issn.1674-490X.2013.05.013

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Objective To evaluate the clinical curative effects of narrow alternating red-blue light in the treatment of facial acne. Methods 60 cases with facial acne,15-35 years old were selected, who were randomly assigned into red light group, blue light group and alternating red-blur light group, each group had 20 cases. To assess the therapeutic effects by observing the skin lesion changes before and after treatment. Results The effective rate of red group was 55%. The effective rates of blue group and red-blue light group were 50%and 80%respectively. Conclusion The treatment of narrow alternating red-blue light in facial acne is markedly faster, has higher safety and it is worthwhile being distributed in clinical practices .

Discussion on surgical treating terrible triad elbow

LIU Jianpeng,FAN Hongqi,LIU Haijin

2013, 30(5):  50-53.  DOI: 10.3969/j.issn.1674-490X.2013.05.014

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Objective To explore the surgical approaches and the main operative points of surgical treatment of the terrible triad elbow. Methods 15 cases were treated with operation. 12 cases were operated with lateral approach combined with anterior median mini incision. 3 cases were operated with lateral approach combined with inner approach .The mini screws and mini plates were used to fix radial head fractures, and external ifxture or external plaster ifxation were used in the treatment. Results The results indicated that the excellent and improved rates were up to 80%. Four cases were improved greatly, 8 cases improved obviously, 2 cases improved, 1 case improved poorly with Mayo elbow score standard. Conclusion The joint capsule of front in elbow, the coronoid process of the ulna and the radial head are very important on the stability of elbow joint. The external ifxture is superior to plaster ifxation in clinical practices.

Clinical analyses and its countermeasures of cesarean section in a second-class hospital

WANG Hongyan,ZHANG Ruihong

2013, 30(5):  54-56.  DOI: 10.3969/j.issn.1674-490X.2013.05.015

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Objective To analyze the cesarean section rates and its causes by contrasting medical data between 2005 to 2012 of a county-level hospital to explore the intervention measures to reduce the cesarean section rate. Methods 3660 cases’ medical records of hospitalized pregnant women were analyzed by retrospective research to calculate annual cesarean section rates and classify its causes of cesarean birth. Results The cesarean section rates of the hospital were 40.83% and 46.83% in 2012 and in 2005 respectively. Compared with the results in 2005, the cesarean causes, the proportion of uterine scar, head disproportion and pregnant women’s own request in 2012 had risen significantly. The composition ratio of breech position, severe pregnancy-induced hypertension, amniotic lfuid shortage and induction failure decreased obviously. Conclusion Compared with that in 2005, the cesarean section rate decline in 2012 is attributed to hospital’s high attention on promoting natural childbirth, obstetric technology improvement, prenatal examination and normative clinical procedures.

Effects of different intervenes on the urinary iodine level in the children aged 8-10 in iodine-deifcient areas

ZHANG Jinguo,XU Jing,YU Jian,ZHANG Baozong,TANG Shuling

2013, 30(5):  57-61.  DOI: 10.3969/j.issn.1674-490X.2013.05.016

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Objective To ifnd out the effects of different intervenes on the urinary iodine level in the children aged 8-10 in iodine-deficient areas. Methods At different times, we took different intervenes in the school children aged 8-10, such as iodized salt intervention, iodized salt with iodized oil intervention, synthesis intervention and so on. With arsenic cerium catalytic spectrophotometric method for testing children's urine, we observed the effects of different interventions on the children's urinary iodine level. Results Before the intervention, Children’s urinary iodine level was low, and it didn’t meet the standard of eliminating national Iodine Deifciency Disorders. However, after the iodized salt intervention, children’s urinary iodine level was improved, and it reached the standard of eliminating national Iodine Deifciency Disorders. After the iodized salt with iodized oil intervention, the children’s urinary iodine level had a further improvement, median urinary iodine was in a suitable range. Adopting comprehensive intervention reinforces children's urinary iodine levels and the median urinary iodine was always in the appropriate range. The differences of urinary iodine level in different intervention stages had statistical significance(P<0.01). Conclusion It is an effective measures to improve the level of children’s iodine nutrition in iodine-deficient area with iodized salt intervention.

Research on the inlfuence of psychological intervention on mental state of the elderly patients with lower limb fracture

SHI Shaobing,WANG Feiran,WU Liping,ZHANG Suxiang

2013, 30(5):  62-65.  DOI: 10.3969/j.issn.1674-490X.2013.05.017

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Objective To investigate the intervention effects of psychological intervention in the elderly patients with lower limb fracture. Methods This study was a self-comparative study. Psychological intervention was used in 68 elderly patients with lower limb fracture. Self-Rating Anxiety Scale (SAS) and self-rating depression scale (SDS) were used to compare the anxiety and depression status of the patients at admission and discharge. The states before and after the intervention were compared with the norms. Results The patients with anxiety and depression at discharge were less than at admission, and the differences were significant(P<0.05). The anxiety and depression scores of patients at discharge were better than at admission, and the differences were signiifcant(P<0.05). Conclusion Psychological intervention can effectively reduce the anxiety and depression of elderly patients with lower limbs fractures, allowing them to responded well to treatment, and it has great value in clinical application.

Clinical observation of Remifentanil in gastroscope examination in the elderly

LI Yajing,TIAN Zili,JIAO Zhengwei,LU Yuxiang,LIU Peng

2013, 30(5):  66-68,74.  DOI: 10.3969/j.issn.1674-490X.2013.05.018

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Objective To observe the safety of gastroscope examination with Remifentanil and nursing approaches in old patients. Methods 100 patients were assigned randomly into two groups. 50 patients in group Propofol were treated with Propofol 1-1.5 mg/kg, and 20-50 mg was added if necessary. Group Remifentanil was intravenously injected with Remifentanil 1 μg/kg before examination, and the medicine was continued injected with the speed of 0.1 μg/(kg·min). In the stage of examination, blood pressure, pulse and blood oxygen were recorded. Results The patients of group Propofol showed a slight inhibition of circulation and respiration, and the numerical values of blood pressure, pulse and blood oxygen were significantly changed in the stage of examination. The patients in group Remifentanil showed no difference. Conclusion The low dosage of Remifentanil can be used safely in gastroscope examination.

The significance of PI3K/Akt/mTOR pathway and autophagy in the proliferation of synovial cells in rheumatoid arthritis

MENG Ming,LIANG Hongge,FANG Hao,ZHOU Lei,FENG Jianyong,DONG Yang,YAN Zhen,CHEN Dongzhi

2013, 30(5):  69-74.  DOI: 10.3969/j.issn.1674-490X.2013.05.019

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Autophagy is a crucial self-saving behavior of mammalian cells in growth and development of organism. Autophagy can keep cells survival via eliminating the metaprotein and necrosis substance then rebuilding the structure of cells in the situation of stress, but it can also lead to typeⅡapoptosis if it occur unduly. PI3K/Akt/mTOR , which involved in multiple physiological and pathological process, is an important signal pathway existing in various cells. The products of activated PI3K/Akt/mTOR pathway will inhibit the cell autophagy. In the early stage of rheumatoid arthritis (RA), the rheumatoid arthritis synovial ifbroblasts (RASF) initiate autophagy in several ways, in order to maintaining the homeostasis of RASF and cell proliferation. With the progress of RA, the activating platelet particles (such as PDGFRα) will suppress autophagy via activating the PI3K/Akt/mTOR signal pathway, thereby prevent RASF from typeⅡapoptosis induced by excessive autophagy, lead to RASF’s continuing proliferation and aggravate the pathogenetic condition. The purpose of this article is to summarize the signiifcance of PI3K/Akt/mTOR signal pathway and autophagy in the persistently proliferation of RASF, discuss the pathogenesis of RA and ifnd new therapeutic targets.

Effects of Integrin-linked kinase in the development of glioma

CUI Dongqiang,WANG Hong,LI Chunhui

2013, 30(5):  79-82.  DOI: 10.3969/j.issn.1674-490X.2013.05.021

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Integrin-Linked kinase (ILK) is widely expressed in multiple tissues cells, closely related to various cellular physiological processes. Recent studies have found that it is involved in tumor cell proliferation, apoptosis, invasion and metastasis, angiogenesis and so on, for the most part to promote the development of tumor. At the same time, the scientists also found in the study that glioma had similar results. This article will discuss about the integrin-linked kinase on glioma in recent years’ research.

Advance on the relationship between COX-2 and gastric cancer

YANG Nan,FENG Yichao

2013, 30(5):  83-86,91.  DOI: 10.3969/j.issn.1674-490X.2013.05.022

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Cyclooxygenase (COX) was the rate-limiting enzyme of the arachidonic acid synthesis of prostaglandins, which had a twofold activity of cyclooxygenase and peroxidase, now it had been confirmed there were two isozymes:COX-1 and COX-2. COX-2 was seen as“rapid response gene”, which was speciifc high expression in gastric cancer, and it was involved in gastric blood vessels, lymphatic formation, inhibition of apoptosis, inhibition of tumor imunity, promoting tumor metastasis, inifltration. Thus, it played an important role in the development of gastric cancer.

Analysis of affecting factors of non(low)-response to hepatitis B vaccine in adults

WANG Jingru,ZHANG Rongzhen,CAO Zhiran

2013, 30(5):  87-91.  DOI: 10.3969/j.issn.1674-490X.2013.05.023

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The immunization of hepatitis B vaccine is the most effective measure to prevent hepatitis B infection, but some adults have no response to the hepatitis B vaccine. This article analyses the affective aspects of non(low)-response after the adult human body vaccination of hepatitis B vaccine, which reviews the human body factors, the vaccine factors and the vaccination factors.

Application of seminar and case teaching approaches in teaching clinical postgraduates majored in respiratory diseases

HUANG Linian,XU Qixia,ZHANG Yong,HU Junfeng,GAO Hua,CHEN Yuqing

2013, 30(5):  92-94.  DOI: 10.3969/j.issn.1674-490X.2013.05.024

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Clinical skills are the main body of clinical medical postgraduates. The students are trained to achieve the ability to obtain medical knowledge, analyze and think individually. The combination of seminar and case teaching methods are applied into teaching process of graduate majored in respiratory diseases, taking students as classroom subjects and case as a guide is able to fully mobilize the initiative of those graduates in their studies, and train their abilities to acquire knowledge.

Exploration and practice on pathophysiology teaching for international students from non-English speaking country

ZHAO Xiaoqi,ZHAO Zigang,JIANG Hua,ZHANG Yuping,DU Shuting,WANG Xiaorong,ZHANG Limin,NIU Chunyu

2013, 30(5):  98-102.  DOI: 10.3969/j.issn.1674-490X.2013.05.026

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The number of international students who study in China has increased year by year, and then the national medical schools have devoted major efforts to developing their international education. We have accumulated some experiences during pathophysiology teaching in these years, and in this article we explore the education route in the aspects of language, culture, theory lesson teaching, experiment teaching, individualized teaching and assessment.

Discussion on the cross-disciplinary joint teaching of nervous system to medical students of the eight-year system

HAO Lu,SUN Jihu,JI Kaihong,LIU Fang

2013, 30(5):  103-106.  DOI: 10.3969/j.issn.1674-490X.2013.05.027

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The cross-disciplinary joint teaching reform of nervous system was continued among the medical students of the eight-year system. It combines the morphology and the functions of the nervous system together. The cross-disciplinary joint teaching improves some teaching steps during the process, and makes it more suitable to the students of small order and in the long educational system. Some opinions and suggestions on the cross-disciplinary joint teaching reform from the perspective of the students are discussed.

Study on application of frame-based approach in teaching Medical Immunology

ZHOU Yan,TANG Yuhong,WU Zhencun,CHENG Jianjun

2013, 30(5):  107-110.  DOI: 10.3969/j.issn.1674-490X.2013.05.028

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Objective To study the effects of the frame-based teaching approach in Medical Immunology. Methods Before teaching, students of experimental class and control class were done questionnaires about basic knowledge of immunology, then analyzed the effects of the frame-based teaching approach in Medical Immunology, according to comparing the students’ achivements of frame-based approach and cramming method between the experimental class and the control class. Results Before teaching, scores of two class showed no difference(P>0.05). After teaching, scores of mid-term and end-term showed normal distribution and were credible, scores of experimental class are higher than that of the control class(P<0.01) which had signiifcant difference. Conclusion The frame-based approach using in teaching Medical Immunology can stimulate the students’ interest and initiative, promote the efifciency and the effects of the study.